Restriction map for pF1KB7522
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaII       AviII       AvrII       
BalI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmAI       BsmBI       
BsmFI       Bsp1407I    BspDI       BspEI       BspMI       
BsrBI       BsrGI       BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HgaI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvaI        
MwoI        NaeI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        ScrFI       SexAI       SfaNI       SfcI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
StyI        SwaI        TfiI        Tth111I     Van91I      
XbaI        XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII          10  CGCG                 CG^CG
AcyI           31  GGCGCC               G[GA]^CG[CT]C
AflII         595  CTTAAG               C^TTAAG
AflIII        543  ACATGT               A^C[GA][CT]GT
AvaI          527  CTCGAG               C^[CT]CG[GA]G
BamHI         680  GGATCC               G^GATCC
BanI           31  GGCGCC               G^G[CT][GA]CC
BbeI           31  GGCGCC               GGCGC^C
BbvI          355  GCTGC                GCAGC(8/12)
BfrI          595  CTTAAG               C^TTAAG
BsaHI          31  GGCGCC               G[GA]^CG[CT]C
BsiYI           5  CCGAGCGCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI            5  CCGAGCGCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          343  GAATGC               GAATGC(1/-1)
BsoBI         527  CTCGAG               C^[CT]CG[GA]G
BspHI         195  TCATGA               T^CATGA
BspLU11I      543  ACATGT               A^CATGT
BsrDI          87  GCAATG               GCAATG(2/0)
BsrFI          28  ACCGGC               [GA]^CCGG[CT]
BstF5I        241  CATCC                GGATG(2/0)
BstUI          10  CGCG                 CG^CG
Cac8I         522  GCGAGC               GC[ACGT]^[ACGT]GC
Cfr10I         28  ACCGGC               [GA]^CCGG[CT]
DrdI          153  GACTGAGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI            2  TGGCCG               [CT]^GGCC[GA]
FauI           11  GCGGG                CCCGC(4/6)
Fnu4HI        355  GCTGC                GC^[ACGT]GC
FokI          241  CATCC                GGATG(9/13)
HaeII          31  GGCGCC               [GA]GCGC^[CT]
HaeIII          3  GGCC                 GG^CC
HapII          29  CCGG                 C^CGG
Hin1I          31  GGCGCC               G[GA]^CG[CT]C
HpaII          29  CCGG                 C^CGG
HphI          112  GGTGA                GGTGA(8/7)
ItaI          355  GCTGC                GC^[ACGT]GC
KasI           31  GGCGCC               G^GCGCC
MspI           29  CCGG                 C^CGG
MvnI           10  CGCG                 CG^CG
NarI           31  GGCGCC               GG^CGCC
PaeR7I        527  CTCGAG               C^TCGAG
RcaI          195  TCATGA               T^CATGA
SfoI           31  GGCGCC               GGC^GCC
TseI          355  GCTGC                G^C[AT]GC
XhoI          527  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AciI           11  GCGG                 CCGC(-3/-1)
              129  GCGG                
BanII         294  GAGCTC               G[GA]GC[CT]^C
              524  GAGCTC              
BbsI          511  GAAGAC               GAAGAC(2/6)
              402  GTCTTC              
BfaI           58  CTAG                 C^TAG
              233  CTAG                
BpuAI         511  GAAGAC               GAAGAC(2/6)
              402  GTCTTC              
BsrI          359  CCAGT                ACTGG(1/-1)
              412  CCAGT               
BstYI         180  GGATCT               [GA]^GATC[CT]
              680  GGATCC              
CfoI            9  GCGC                 GCG^C
               32  GCGC                
DdeI          155  CTGAG                C^T[ACGT]AG
              290  CTGAG               
DpnII         181  GATC                 ^GATC
              681  GATC                
EarI          107  GAAGAG               CTCTTC(1/4)
              397  GAAGAG              
HhaI            9  GCGC                 GCG^C
               32  GCGC                
HinP1I          9  GCGC                 G^CGC
               32  GCGC                
HinfI         141  GACTC                G^A[ACGT]TC
              400  GAGTC               
Ksp632I       107  GAAGAG               CTCTTC(1/4)
              397  GAAGAG              
MaeI           58  CTAG                 C^TAG
              233  CTAG                
MboI          181  GATC                 ^GATC
              681  GATC                
MflI          180  GGATCT               [GA]^GATC[CT]
              680  GGATCC              
MslI           45  CACAGGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
               83  CACAGCAATG          
MspA1I        353  CAGCTG               C[AC]G^C[GT]G
              430  CAGCTG              
NdeII         181  GATC                 ^GATC
              681  GATC                
PleI          141  GACTC                GAGTC(4/5)
              400  GAGTC               
PvuII         353  CAGCTG               CAG^CTG
              430  CAGCTG              
SacI          294  GAGCTC               GAGCT^C
              524  GAGCTC              
Sau3AI        181  GATC                 ^GATC
              681  GATC                
SspI           74  AATATT               AAT^ATT
              190  AATATT              
Tsp45I         81  GTCAC                ^GT[GC]AC
              743  GTGAC               
Tsp509I       454  AATT                 ^AATT
              657  AATT                
XhoII         180  GGATCT               [GA]^GATC[CT]
              680  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AfaI        AluI        AlwI        AspHI       BmyI        
BsiHKAI     Bsp1286I    MaeIII      MboII       MnlI        
MseI        NlaIII      NlaIV       NspI        RsaI        
SmlI        TaqI        Tru9I       TspRI       TthHB8I     
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