Restriction map for pF1KB7522
 (Linear) (Six-base) MAPSORT of: KIBB7522.seq  Check: 3669  from: 1  to: 747



KIBB7522.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     658     724     747 
   Size:        203     455      66      23

  Fragments arranged by size: 

                 455     203      66      23
 
AflII C'TTAA_G

Cuts at:      0     595     747 
   Size:        595     152
 
AflIII A'CryG_T

Cuts at:      0     543     747 
   Size:        543     204
 
AleI CACnn'nnGTG

Cuts at:      0      49     747 
   Size:         49     698
 
AvaI C'yCGr_G

Cuts at:      0     527     747 
   Size:        527     220
 
BamHI G'GATC_C

Cuts at:      0     680     747 
   Size:        680      67
 
BanI G'GyrC_C

Cuts at:      0      31     747 
   Size:         31     716
 
BanII G_rGCy'C

Cuts at:      0     298     528     747 
   Size:        298     230     219

  Fragments arranged by size: 

                 298     230     219
 
BbeI G_GCGC'C

Cuts at:      0      35     747 
   Size:         35     712
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     395     518     747 
   Size:        395     123     229

  Fragments arranged by size: 

                 395     229     123
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     747 
   Size:        128     619
 
BsaHI Gr'CG_yC

Cuts at:      0      32     747 
   Size:         32     715
 
BsiHKAI G_wGCw'C

Cuts at:      0     231     298     528     747 
   Size:        231      67     230     219

  Fragments arranged by size: 

                 231     230     219      67
 
BsmI GAATG_Cn'

Cuts at:      0     349     747 
   Size:        349     398
 
Bsp1286I G_dGCh'C

Cuts at:      0     231     298     528     747 
   Size:        231      67     230     219

  Fragments arranged by size: 

                 231     230     219      67
 
BspHI T'CATG_A

Cuts at:      0     195     747 
   Size:        195     552
 
BsrDI GCAATG_nn'

Cuts at:      0      94     747 
   Size:         94     653
 
BsrFI r'CCGG_y

Cuts at:      0      28     747 
   Size:         28     719
 
BstYI r'GATC_y

Cuts at:      0     180     680     747 
   Size:        180     500      67

  Fragments arranged by size: 

                 500     180      67
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     159     747 
   Size:        159     588
 
EaeI y'GGCC_r

Cuts at:      0       2     747 
   Size:          2     745
 
EarI CTCTTCn'nnn_

Cuts at:      0     102     392     747 
   Size:        102     290     355

  Fragments arranged by size: 

                 355     290     102
 
EcoICRI GAG'CTC

Cuts at:      0     296     526     747 
   Size:        296     230     221

  Fragments arranged by size: 

                 296     230     221
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     658     724     747 
   Size:        203     455      66      23

  Fragments arranged by size: 

                 455     203      66      23
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     663     747 
   Size:        663      84
 
HaeII r_GCGC'y

Cuts at:      0      35     747 
   Size:         35     712
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     283     295     315     327     747 
   Size:        283      12      20      12     420

  Fragments arranged by size: 

                 420     283      20      12      12
 
KasI G'GCGC_C

Cuts at:      0      31     747 
   Size:         31     716
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     243     747 
   Size:        243     504
 
MslI CAynn'nnrTG

Cuts at:      0      49      87     747 
   Size:         49      38     660

  Fragments arranged by size: 

                 660      49      38
 
MspA1I CmG'CkG

Cuts at:      0     355     432     747 
   Size:        355      77     315

  Fragments arranged by size: 

                 355     315      77
 
NarI GG'CG_CC

Cuts at:      0      32     747 
   Size:         32     715
 
NspI r_CATG'y

Cuts at:      0     375     547     558     747 
   Size:        375     172      11     189

  Fragments arranged by size: 

                 375     189     172      11
 
PciI A'CATG_T

Cuts at:      0     543     747 
   Size:        543     204
 
PspXI vC'TCGA_Gb

Cuts at:      0     527     747 
   Size:        527     220
 
PvuII CAG'CTG

Cuts at:      0     355     432     747 
   Size:        355      77     315

  Fragments arranged by size: 

                 355     315      77
 
SacI G_AGCT'C

Cuts at:      0     298     528     747 
   Size:        298     230     219

  Fragments arranged by size: 

                 298     230     219
 
SfoI GGC'GCC

Cuts at:      0      33     747 
   Size:         33     714
 
SmlI C'TyrA_G

Cuts at:      0     143     527     595     747 
   Size:        143     384      68     152

  Fragments arranged by size: 

                 384     152     143      68
 
SspI AAT'ATT

Cuts at:      0      76     192     747 
   Size:         76     116     555

  Fragments arranged by size: 

                 555     116      76
 
TatI w'GTAC_w

Cuts at:      0     713     747 
   Size:        713      34
 
XhoI C'TCGA_G

Cuts at:      0     527     747 
   Size:        527     220
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AleI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BpuEI    BsaHI  BsiHKAI     BsmI Bsp1286I    BspHI
    BsrDI    BsrFI    BstYI     DrdI     EaeI     EarI  EcoICRI  Eco57MI
     FalI    HaeII    Hin4I     KasI     MmeI     MslI   MspA1I     NarI
     NspI     PciI    PspXI    PvuII     SacI     SfoI     SmlI     SspI
     TatI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI     PsrI     PstI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI
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