Restriction map for pF1KE0779
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# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AlwI        AocI        ApaI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbrPI       BbsI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaI        BsaWI       BseAI       
BsgI        BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrGI       BsrI        Bst1107I    
BstAPI      BstBI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco81I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
MaeI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SalI        SbfI        ScaI        
SexAI       SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TfiI        Tru9I       Tsp509I     Tth111I     
Van91I      XbaI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Alw44I        478  GTGCAC               G^TGCAC
AlwNI         151  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       946  AGCGCT               AGC^GCT
ApaLI         478  GTGCAC               G^TGCAC
BanI          977  GGCGCC               G^G[CT][GA]CC
BbeI          977  GGCGCC               GGCGC^C
BbvCI         868  CCTCAGC              CCTCAGC(-5/-2)
BsaBI         954  GATCGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI         896  CGGCCG               CG[GA][CT]^CG
BssSI         618  CACGAG               CACGAG(-5/-1)
BstEII        234  GGTCACC              G^GT[ACGT]ACC
BstPI         234  GGTCACC              G^GT[ACGT]ACC
DpnII         954  GATC                 ^GATC
EagI          896  CGGCCG               C^GGCCG
EclXI         896  CGGCCG               C^GGCCG
Eco47III      946  AGCGCT               AGC^GCT
Eco52I        896  CGGCCG               C^GGCCG
EcoO65I       234  GGTCACC              G^GT[ACGT]ACC
EcoT14I       452  CCAAGG               C^C[AT][AT]GG
HinfI         382  GAGTC                G^A[ACGT]TC
KasI          977  GGCGCC               G^GCGCC
MamI          954  GATCGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          954  GATC                 ^GATC
NarI          977  GGCGCC               GG^CGCC
NdeII         954  GATC                 ^GATC
PleI          382  GAGTC                GAGTC(4/5)
PshAI         153  GACCCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         496  CAGCTG               CAG^CTG
SapI          756  GCTCTTC              GCTCTTC(1/4)
Sau3AI        954  GATC                 ^GATC
SfaNI         377  GCATC                GCATC(5/9)
SfcI          396  CTACAG               C^T[GA][CT]AG
SfiI          888  GGCCCCCGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          977  GGCGCC               GGC^GCC
SmaI          857  CCCGGG               CCC^GGG
StuI          622  AGGCCT               AGG^CCT
StyI          452  CCAAGG               C^C[AT][AT]GG
TaqI          380  TCGA                 T^CGA
TthHB8I       380  TCGA                 T^CGA
XcmI          207  CCAGGGTCGCTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         857  CCCGGG               C^CCGGG
XmaI          857  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BcgI           37  GCAGGGGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              371  GCATTGGCATCG        
BglI          493  GCCCAGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              889  GCCCCCGCGGC         
BsrBI         697  CCGCTC               CCGCTC(-3/-3)
             1019  GAGCGG              
EarI          757  CTCTTC               CTCTTC(1/4)
             1027  GAAGAG              
EcoNI         201  CCTGGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              798  CCTGGCCGAGG         
HphI          236  TCACC                GGTGA(8/7)
              930  TCACC               
Ksp632I       757  CTCTTC               CTCTTC(1/4)
             1027  GAAGAG              
MaeII         292  ACGT                 A^CGT
              563  ACGT                
MaeIII        235  GTCAC                ^GT[ACGT]AC
              358  GTGAC               
MboII        1027  GAAGA                GAAGA(8/7)
              758  TCTTC               
NaeI           27  GCCGGC               GCC^GGC
              587  GCCGGC              
NgoMIV         27  GCCGGC               G^CCGGC
              587  GCCGGC              
PpuMI         428  GGGACCT              [GA]G^G[AT]CC[CT]
              545  GGGACCT             
Tsp45I        235  GTCAC                ^GT[GC]AC
              358  GTGAC               
TspRI         266  CACTG                CA[GC]TG[ACGT][ACGT]^
             1013  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AspHI       AvaI        AvaII       BanII       BbvI        
BcnI        BmyI        BpmI        BsaHI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BsrFI       BssHII      BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DraII       DsaI        
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HpaII       ItaI        
KspI        MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NlaIII      NlaIV       
RsaI        SacI        SacII       Sau96I      ScrFI       
TseI        
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