Restriction map for pF1KE0779
 (Linear) (Six-base) MAPSORT of: KIEE0779.seq  Check: 2732  from: 1  to: 1044



KIEE0779.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     571    1044 
   Size:        289     282     473

  Fragments arranged by size: 

                 473     289     282
 
AfeI AGC'GCT

Cuts at:      0     948    1044 
   Size:        948      96
 
AlwNI CAG_nnn'CTG

Cuts at:      0     156    1044 
   Size:        156     888
 
ApaLI G'TGCA_C

Cuts at:      0     478    1044 
   Size:        478     566
 
AvaI C'yCGr_G

Cuts at:      0     676     703     857    1044 
   Size:        676      27     154     187

  Fragments arranged by size: 

                 676     187     154      27
 
BanI G'GyrC_C

Cuts at:      0     977    1044 
   Size:        977      67
 
BanII G_rGCy'C

Cuts at:      0      95     182     332     416     683    1044 
   Size:         95      87     150      84     267     361

  Fragments arranged by size: 

                 361     267     150      95      87      84
 
BbeI G_GCGC'C

Cuts at:      0     981    1044 
   Size:        981      63
 
BbvCI CC'TCA_GC

Cuts at:      0     869    1044 
   Size:        869     175
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      26      60     360     394    1044 
   Size:         26      34     300      34     650

  Fragments arranged by size: 

                 650     300      34      34      26
 
BglI GCCn_nnn'nGGC

Cuts at:      0     499     895    1044 
   Size:        499     396     149

  Fragments arranged by size: 

                 499     396     149
 
Bme1580I G_kGCm'C

Cuts at:      0     482    1044 
   Size:        482     562
 
BmgBI CAC'GTC

Cuts at:      0     293    1044 
   Size:        293     751
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     145     532    1005    1044 
   Size:         79      66     387     473      39

  Fragments arranged by size: 

                 473     387      79      66      39
 
Bpu10I CC'TnA_GC

Cuts at:      0     869    1044 
   Size:        869     175
 
BsaBI GATnn'nnATC

Cuts at:      0     958    1044 
   Size:        958      86
 
BsaHI Gr'CG_yC

Cuts at:      0     734     749     844     978    1044 
   Size:        734      15      95     134      66

  Fragments arranged by size: 

                 734     134      95      66      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73      76     103     757     957    1044 
   Size:         73       3      27     654     200      87

  Fragments arranged by size: 

                 654     200      87      73      27       3
 
BseYI C'CCAG_C

Cuts at:      0     336     494     744    1036    1044 
   Size:        336     158     250     292       8

  Fragments arranged by size: 

                 336     292     250     158       8
 
BsiEI CG_ry'CG

Cuts at:      0     899    1044 
   Size:        899     145
 
BsiHKAI G_wGCw'C

Cuts at:      0      95     150     182     482     683     971    1044 
   Size:         95      55      32     300     201     288      73

  Fragments arranged by size: 

                 300     288     201      95      73      55      32
 
Bsp1286I G_dGCh'C

Cuts at:      0      95     150     182     332     416     482     683     971 
   Size:         95      55      32     150      84      66     201     288

Cuts at:    971    1044 
   Size:         73

  Fragments arranged by size: 

                 288     201     150      95      84      73      66      55
                  32
 
BsrBI CCG'CTC

Cuts at:      0     699    1021    1044 
   Size:        699     322      23

  Fragments arranged by size: 

                 699     322      23
 
BsrFI r'CCGG_y

Cuts at:      0      27     587     791    1044 
   Size:         27     560     204     253

  Fragments arranged by size: 

                 560     253     204      27
 
BssHII G'CGCG_C

Cuts at:      0     119     341     686    1044 
   Size:        119     222     345     358

  Fragments arranged by size: 

                 358     345     222     119
 
BssSI C'ACGA_G

Cuts at:      0     618    1044 
   Size:        618     426
 
BstEII G'GTnAC_C

Cuts at:      0     234    1044 
   Size:        234     810
 
BtgI C'CryG_G

Cuts at:      0     300     473     528     893    1044 
   Size:        300     173      55     365     151

  Fragments arranged by size: 

                 365     300     173     151      55
 
BtsI GCAGTG_nn'

Cuts at:      0    1019    1044 
   Size:       1019      25
 
EaeI y'GGCC_r

Cuts at:      0     219     612     647     800     896    1044 
   Size:        219     393      35     153      96     148

  Fragments arranged by size: 

                 393     219     153     148      96      35
 
EagI C'GGCC_G

Cuts at:      0     896    1044 
   Size:        896     148
 
EarI CTCTTCn'nnn_

Cuts at:      0     763    1022    1044 
   Size:        763     259      22

  Fragments arranged by size: 

                 763     259      22
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      46     495    1021    1044 
   Size:         46     449     526      23

  Fragments arranged by size: 

                 526     449      46      23
 
EcoICRI GAG'CTC

Cuts at:      0      93     180     681    1044 
   Size:         93      87     501     363

  Fragments arranged by size: 

                 501     363      93      87
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     145     289     532     571    1005    1044 
   Size:         79      66     144     243      39     434      39

  Fragments arranged by size: 

                 434     243     144      79      66      39      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     205     802    1044 
   Size:        205     597     242

  Fragments arranged by size: 

                 597     242     205
 
EcoO109I rG'GnC_Cy

Cuts at:      0     429     546     708    1044 
   Size:        429     117     162     336

  Fragments arranged by size: 

                 429     336     162     117
 
HaeII r_GCGC'y

Cuts at:      0     594     745     950     981    1044 
   Size:        594     151     205      31      63

  Fragments arranged by size: 

                 594     205     151      63      31
 
KasI G'GCGC_C

Cuts at:      0     977    1044 
   Size:        977      67
 
MspA1I CmG'CkG

Cuts at:      0     475     485     498     530     895     993    1044 
   Size:        475      10      13      32     365      98      51

  Fragments arranged by size: 

                 475     365      98      51      32      13      10
 
NaeI GCC'GGC

Cuts at:      0      29     589    1044 
   Size:         29     560     455

  Fragments arranged by size: 

                 560     455      29
 
NarI GG'CG_CC

Cuts at:      0     978    1044 
   Size:        978      66
 
NgoMIV G'CCGG_C

Cuts at:      0      27     587    1044 
   Size:         27     560     457

  Fragments arranged by size: 

                 560     457      27
 
PasI CC'CwG_GG

Cuts at:      0     201     785    1044 
   Size:        201     584     259

  Fragments arranged by size: 

                 584     259     201
 
PpuMI rG'GwC_Cy

Cuts at:      0     429     546    1044 
   Size:        429     117     498

  Fragments arranged by size: 

                 498     429     117
 
PshAI GACnn'nnGTC

Cuts at:      0     157    1044 
   Size:        157     887
 
PvuII CAG'CTG

Cuts at:      0     498    1044 
   Size:        498     546
 
SacI G_AGCT'C

Cuts at:      0      95     182     683    1044 
   Size:         95      87     501     361

  Fragments arranged by size: 

                 501     361      95      87
 
SacII CC_GC'GG

Cuts at:      0     476     531     896    1044 
   Size:        476      55     365     148

  Fragments arranged by size: 

                 476     365     148      55
 
SapI GCTCTTCn'nnn_

Cuts at:      0     763    1044 
   Size:        763     281
 
SfcI C'TryA_G

Cuts at:      0     396    1044 
   Size:        396     648
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     895    1044 
   Size:        895     149
 
SfoI GGC'GCC

Cuts at:      0     979    1044 
   Size:        979      65
 
SmaI CCC'GGG

Cuts at:      0     859    1044 
   Size:        859     185
 
StuI AGG'CCT

Cuts at:      0     624    1044 
   Size:        624     420
 
StyI C'CwwG_G

Cuts at:      0     452    1044 
   Size:        452     592
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     253     619    1044 
   Size:        253     366     425

  Fragments arranged by size: 

                 425     366     253
 
TatI w'GTAC_w

Cuts at:      0     515    1044 
   Size:        515     529
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     214    1044 
   Size:        214     830
 
XmaI C'CCGG_G

Cuts at:      0     857    1044 
   Size:        857     187
 

 Enzymes that do cut:

     AcuI     AfeI    AlwNI    ApaLI     AvaI     BanI    BanII     BbeI
    BbvCI     BcgI     BglI Bme1580I    BmgBI     BpmI   Bpu10I    BsaBI
    BsaHI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BsrBI    BsrFI
   BssHII    BssSI   BstEII     BtgI     BtsI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII     KasI   MspA1I
     NaeI     NarI   NgoMIV     PasI    PpuMI    PshAI    PvuII     SacI
    SacII     SapI     SfcI     SfiI     SfoI     SmaI     StuI     StyI
    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BciVI     BclI    BfrBI
    BglII     BlpI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaWI    BsaXI     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrGI   BstAPI    BstBI    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SalI    SanDI     SbfI     ScaI
    SexAI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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