Restriction map for pF1KB0928
 (Linear) (Six-base) MAPSORT of: KIBB0928.seq  Check: 8750  from: 1  to: 1092



KIBB0928.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     271    1092 
   Size:        271     821
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     992    1092 
   Size:        992     100
 
AfeI AGC'GCT

Cuts at:      0     650     670    1092 
   Size:        650      20     422

  Fragments arranged by size: 

                 650     422      20
 
AflIII A'CryG_T

Cuts at:      0     638    1092 
   Size:        638     454
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     436    1092 
   Size:        436     656
 
AlwNI CAG_nnn'CTG

Cuts at:      0     744     844     859     877    1092 
   Size:        744     100      15      18     215

  Fragments arranged by size: 

                 744     215     100      18      15
 
ApaLI G'TGCA_C

Cuts at:      0     118    1092 
   Size:        118     974
 
ApoI r'AATT_y

Cuts at:      0    1023    1092 
   Size:       1023      69
 
AvaI C'yCGr_G

Cuts at:      0      29     460    1092 
   Size:         29     431     632

  Fragments arranged by size: 

                 632     431      29
 
BanII G_rGCy'C

Cuts at:      0     928    1092 
   Size:        928     164
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     134    1092 
   Size:        134     958
 
BbvCI CC'TCA_GC

Cuts at:      0     143    1092 
   Size:        143     949
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      34      68    1092 
   Size:         34      34    1024

  Fragments arranged by size: 

                1024      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1072    1092 
   Size:       1072      20
 
Bme1580I G_kGCm'C

Cuts at:      0     122     509    1092 
   Size:        122     387     583

  Fragments arranged by size: 

                 583     387     122
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     943    1092 
   Size:        943     149
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      72    1092 
   Size:         72    1020
 
Bpu10I CC'TnA_GC

Cuts at:      0     143     448    1092 
   Size:        143     305     644

  Fragments arranged by size: 

                 644     305     143
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     464    1092 
   Size:        464     628
 
BsaAI yAC'GTr

Cuts at:      0     426    1092 
   Size:        426     666
 
BsaWI w'CCGG_w

Cuts at:      0     306    1092 
   Size:        306     786
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     778     808    1092 
   Size:        778      30     284

  Fragments arranged by size: 

                 778     284      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     310    1092 
   Size:        310     782
 
BseYI C'CCAG_C

Cuts at:      0     749    1092 
   Size:        749     343
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     249     448    1092 
   Size:        148     101     199     644

  Fragments arranged by size: 

                 644     199     148     101
 
BsiEI CG_ry'CG

Cuts at:      0    1071    1092 
   Size:       1071      21
 
BsiHKAI G_wGCw'C

Cuts at:      0     122    1092 
   Size:        122     970
 
BsiWI C'GTAC_G

Cuts at:      0     592     641    1092 
   Size:        592      49     451

  Fragments arranged by size: 

                 592     451      49
 
BsmI GAATG_Cn'

Cuts at:      0     425    1092 
   Size:        425     667
 
Bsp1286I G_dGCh'C

Cuts at:      0     122     509     928    1092 
   Size:        122     387     419     164

  Fragments arranged by size: 

                 419     387     164     122
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      32     170     271    1092 
   Size:         32     138     101     821

  Fragments arranged by size: 

                 821     138     101      32
 
BsrBI CCG'CTC

Cuts at:      0     357     479    1053    1092 
   Size:        357     122     574      39

  Fragments arranged by size: 

                 574     357     122      39
 
BsrFI r'CCGG_y

Cuts at:      0     443    1092 
   Size:        443     649
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     367     877    1092 
   Size:        367     510     215

  Fragments arranged by size: 

                 510     367     215
 
Bsu36I CC'TnA_GG

Cuts at:      0     837    1092 
   Size:        837     255
 
BtgI C'CryG_G

Cuts at:      0     776    1092 
   Size:        776     316
 
BtsI GCAGTG_nn'

Cuts at:      0     377     593     704    1092 
   Size:        377     216     111     388

  Fragments arranged by size: 

                 388     377     216     111
 
EaeI y'GGCC_r

Cuts at:      0     779    1092 
   Size:        779     313
 
EarI CTCTTCn'nnn_

Cuts at:      0      67    1092 
   Size:         67    1025
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     992    1092 
   Size:        992     100
 
EcoO109I rG'GnC_Cy

Cuts at:      0     387    1092 
   Size:        387     705
 
EcoRI G'AATT_C

Cuts at:      0    1023    1092 
   Size:       1023      69
 
HaeII r_GCGC'y

Cuts at:      0     652     672    1092 
   Size:        652      20     420

  Fragments arranged by size: 

                 652     420      20
 
HindIII A'AGCT_T

Cuts at:      0     175    1004    1092 
   Size:        175     829      88

  Fragments arranged by size: 

                 829     175      88
 
MluI A'CGCG_T

Cuts at:      0     638    1092 
   Size:        638     454
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     942    1092 
   Size:        942     150
 
MscI TGG'CCA

Cuts at:      0     781    1092 
   Size:        781     311
 
MslI CAynn'nnrTG

Cuts at:      0     196     989    1092 
   Size:        196     793     103

  Fragments arranged by size: 

                 793     196     103
 
MspA1I CmG'CkG

Cuts at:      0     142     706     712     753     856     877    1092 
   Size:        142     564       6      41     103      21     215

  Fragments arranged by size: 

                 564     215     142     103      41      21       6
 
NaeI GCC'GGC

Cuts at:      0     445    1092 
   Size:        445     647
 
NcoI C'CATG_G

Cuts at:      0     776    1092 
   Size:        776     316
 
NgoMIV G'CCGG_C

Cuts at:      0     443    1092 
   Size:        443     649
 
NspI r_CATG'y

Cuts at:      0     244    1092 
   Size:        244     848
 
PmlI CAC'GTG

Cuts at:      0     426    1092 
   Size:        426     666
 
PstI C_TGCA'G

Cuts at:      0     711     867     894    1092 
   Size:        711     156      27     198

  Fragments arranged by size: 

                 711     198     156      27
 
PvuI CG_AT'CG

Cuts at:      0    1071    1092 
   Size:       1071      21
 
PvuII CAG'CTG

Cuts at:      0     142     706     712     753     856    1092 
   Size:        142     564       6      41     103     236

  Fragments arranged by size: 

                 564     236     142     103      41       6
 
SfcI C'TryA_G

Cuts at:      0      38     707     863     890     941    1092 
   Size:         38     669     156      27      51     151

  Fragments arranged by size: 

                 669     156     151      51      38      27
 
SmaI CCC'GGG

Cuts at:      0      31     462    1092 
   Size:         31     431     630

  Fragments arranged by size: 

                 630     431      31
 
StyI C'CwwG_G

Cuts at:      0     776    1092 
   Size:        776     316
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     574    1092 
   Size:        574     518
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     778     810    1092 
   Size:        778      32     282

  Fragments arranged by size: 

                 778     282      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     744    1092 
   Size:        744     348
 
XmaI C'CCGG_G

Cuts at:      0      29     460    1092 
   Size:         29     431     632

  Fragments arranged by size: 

                 632     431      29
 
XmnI GAAnn'nnTTC

Cuts at:      0      10    1092 
   Size:         10    1082
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AlfI    AlwNI    ApaLI     ApoI
     AvaI    BanII     BbsI    BbvCI     BcgI     BglI Bme1580I     BmrI
     BplI   Bpu10I     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspMI    BsrBI
    BsrFI   BstAPI   Bsu36I     BtgI     BtsI     EaeI     EarI  Eco57MI
 EcoO109I    EcoRI    HaeII  HindIII     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NspI     PmlI     PstI     PvuI
    PvuII     SfcI     SmaI     StyI    TaqII     TstI  Tth111I     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BanI     BbeI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI
     BmtI     BpmI    BpuEI    BsaBI    BsaHI    BsmBI    BspEI    BspHI
    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     XbaI
     XcmI     XhoI     ZraI
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