Restriction map for pF1KE0664
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaII       AviII       AvrII       
BalI        BamHI       BbrPI       BbsI        BbvCI       
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsgI        BsiWI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DrdI        Eam1105I    EarI        
Eco47III    Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
Ksp632I     MaeII       MamI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MunI        NdeI        NheI        NsiI        
NspI        NspV        PacI        PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       Tsp509I     
Tth111I     XbaI        XcmI        XhoII       


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# ONE CUT ENZYMES (   33 enzymes) #
###################################

Asp700I        24  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         109  GTGCTC               G[AT]GC[AT]^C
BbeI          871  GGCGCC               GGCGC^C
BcgI          314  CGAGGTTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1135  CTAG                 C^TAG
BsiHKAI       109  GTGCTC               G[AT]GC[AT]^C
BsmI          527  GAATGC               GAATGC(1/-1)
BspMI         413  ACCTGC               ACCTGC(4/8)
BsrI          737  CCAGT                ACTGG(1/-1)
BstXI         233  CCATCAAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII       1021  CACCGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          963  CGGCCG               C^GGCCG
EclXI         963  CGGCCG               C^GGCCG
Eco52I        963  CGGCCG               C^GGCCG
HaeII         871  GGCGCC               [GA]GCGC^[CT]
HgaI          181  GACGC                GACGC(5/10)
HinfI        1087  GATTC                G^A[ACGT]TC
KasI          871  GGCGCC               G^GCGCC
MaeI         1135  CTAG                 C^TAG
NaeI          275  GCCGGC               GCC^GGC
NarI          871  GGCGCC               GG^CGCC
NcoI         1058  CCATGG               C^CATGG
NgoMIV        275  GCCGGC               G^CCGGC
NotI          962  GCGGCCGC             GC^GGCCGC
NruI          795  TCGCGA               TCG^CGA
PflMI        1036  CCAGATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         748  GACGACTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1107  CTGCAG               CTGCA^G
SfoI          871  GGCGCC               GGC^GCC
TfiI         1087  GATTC                G^A[AT]TC
TspRI         738  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I       1036  CCAGATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI           24  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcyI          181  GACGCC               G[GA]^CG[CT]C
              871  GGCGCC              
AfaI          711  GTAC                 GT^AC
              930  GTAC                
AlwI           12  GGATC                GGATC(4/5)
              813  GGATC               
BanI          871  GGCGCC               G^G[CT][GA]CC
             1113  GGTGCC              
BglI          275  GCCGGCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1097  GCCGGTCAGGC         
BpmI          423  CTCCAG               CTGGAG(16/14)
              735  CTCCAG              
BsaHI         181  GACGCC               G[GA]^CG[CT]C
              871  GGCGCC              
DdeI          570  CTCAG                C^T[ACGT]AG
              820  CTGAG               
EcoT14I       185  CCAAGG               C^C[AT][AT]GG
             1058  CCATGG              
Hin1I         181  GACGCC               G[GA]^CG[CT]C
              871  GGCGCC              
HphI          161  TCACC                GGTGA(8/7)
              943  TCACC               
MslI          234  CATCAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              733  CACTCCAGTG          
PaeR7I        660  CTCGAG               C^TCGAG
             1008  CTCGAG              
RsaI          711  GTAC                 GT^AC
              930  GTAC                
StyI          185  CCAAGG               C^C[AT][AT]GG
             1058  CCATGG              
Tsp45I        160  GTCAC                ^GT[GC]AC
              803  GTGAC               
XhoI          660  CTCGAG               C^TCGAG
             1008  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AccII       AciI        AluI        ApaI        AvaI        
BanII       BbvI        BcnI        BmyI        BsaJI       
BseRI       BsiEI       BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BsrFI       
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HpaII       ItaI        KspI        MaeIII      
MboI        MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       SacII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        TaqI        
TseI        TthHB8I     XmaCI       XmaI        
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