Restriction map for pF1KE0664
 (Linear) (Six-base) MAPSORT of: KIEE0664.seq  Check: 9899  from: 1  to: 1176



KIEE0664.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AleI CACnn'nnGTG

Cuts at:      0     737    1176 
   Size:        737     439
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     199     231    1176 
   Size:        199      32     945

  Fragments arranged by size: 

                 945     199      32
 
ApaI G_GGCC'C

Cuts at:      0     286     380     432     505     543     625    1176 
   Size:        286      94      52      73      38      82     551

  Fragments arranged by size: 

                 551     286      94      82      73      52      38
 
AvaI C'yCGr_G

Cuts at:      0      18     312     368     660     683    1008    1176 
   Size:         18     294      56     292      23     325     168

  Fragments arranged by size: 

                 325     294     292     168      56      23      18
 
BanI G'GyrC_C

Cuts at:      0     871    1113    1176 
   Size:        871     242      63

  Fragments arranged by size: 

                 871     242      63
 
BanII G_rGCy'C

Cuts at:      0     286     380     432     505     543     625     845    1049 
   Size:        286      94      52      73      38      82     220     204

Cuts at:   1049    1176 
   Size:        127

  Fragments arranged by size: 

                 286     220     204     127      94      82      73      52
                  38
 
BbeI G_GCGC'C

Cuts at:      0     875    1176 
   Size:        875     301
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     303     337    1176 
   Size:        303      34     839

  Fragments arranged by size: 

                 839     303      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     281    1103    1176 
   Size:        281     822      73

  Fragments arranged by size: 

                 822     281      73
 
Bme1580I G_kGCm'C

Cuts at:      0     286     380     432     505     543     625    1118    1176 
   Size:        286      94      52      73      38      82     493      58

  Fragments arranged by size: 

                 493     286      94      82      73      58      52      38
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     657    1176 
   Size:        657     519
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     408     720    1176 
   Size:        408     312     456

  Fragments arranged by size: 

                 456     408     312
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     780    1176 
   Size:        780     396
 
BsaHI Gr'CG_yC

Cuts at:      0     182     872    1176 
   Size:        182     690     304

  Fragments arranged by size: 

                 690     304     182
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     718     748    1176 
   Size:        718      30     428

  Fragments arranged by size: 

                 718     428      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     923     926     940    1169    1172    1176 
   Size:        923       3      14     229       3       4

  Fragments arranged by size: 

                 923     229      14       4       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1079    1176 
   Size:       1079      97
 
BsiEI CG_ry'CG

Cuts at:      0     309     777     801     966    1002    1176 
   Size:        309     468      24     165      36     174

  Fragments arranged by size: 

                 468     309     174     165      36      24
 
BsiHKAI G_wGCw'C

Cuts at:      0     113    1176 
   Size:        113    1063
 
BsmI GAATG_Cn'

Cuts at:      0     533    1176 
   Size:        533     643
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     554     644     773    1176 
   Size:        554      90     129     403

  Fragments arranged by size: 

                 554     403     129      90
 
Bsp1286I G_dGCh'C

Cuts at:      0     113     286     380     432     505     543     625     845 
   Size:        113     173      94      52      73      38      82     220

Cuts at:    845    1049    1118    1176 
   Size:        204      69      58

  Fragments arranged by size: 

                 220     204     173     113      94      82      73      69
                  58      52      38
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     422    1176 
   Size:        422     754
 
BsrFI r'CCGG_y

Cuts at:      0     258     275    1097    1176 
   Size:        258      17     822      79

  Fragments arranged by size: 

                 822     258      79      17
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     240    1176 
   Size:        240     936
 
BtgI C'CryG_G

Cuts at:      0     303     333     352     625    1058    1176 
   Size:        303      30      19     273     433     118

  Fragments arranged by size: 

                 433     303     273     118      30      19
 
DraIII CAC_nnn'GTG

Cuts at:      0    1026    1176 
   Size:       1026     150
 
EaeI y'GGCC_r

Cuts at:      0      93     545     729     963     990    1176 
   Size:         93     452     184     234      27     186

  Fragments arranged by size: 

                 452     234     186     184      93      27
 
EagI C'GGCC_G

Cuts at:      0     963    1176 
   Size:        963     213
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     418    1139    1176 
   Size:        418     721      37

  Fragments arranged by size: 

                 721     418      37
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     408     720    1176 
   Size:        408     312     456

  Fragments arranged by size: 

                 456     408     312
 
EcoO109I rG'GnC_Cy

Cuts at:      0      39     282     428     429     539     540    1176 
   Size:         39     243     146       1     110       1     636

  Fragments arranged by size: 

                 636     243     146     110      39       1       1
 
HaeII r_GCGC'y

Cuts at:      0     875    1176 
   Size:        875     301
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     553     585     643     675     991    1023    1176 
   Size:        553      32      58      32     316      32     153

  Fragments arranged by size: 

                 553     316     153      58      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     871    1176 
   Size:        871     305
 
MslI CAynn'nnrTG

Cuts at:      0     238     737    1176 
   Size:        238     499     439

  Fragments arranged by size: 

                 499     439     238
 
MspA1I CmG'CkG

Cuts at:      0     305     335     354     518     594     627     959    1176 
   Size:        305      30      19     164      76      33     332     217

  Fragments arranged by size: 

                 332     305     217     164      76      33      30      19
            
 
NaeI GCC'GGC

Cuts at:      0     277    1176 
   Size:        277     899
 
NarI GG'CG_CC

Cuts at:      0     872    1176 
   Size:        872     304
 
NcoI C'CATG_G

Cuts at:      0    1058    1176 
   Size:       1058     118
 
NgoMIV G'CCGG_C

Cuts at:      0     275    1176 
   Size:        275     901
 
NotI GC'GGCC_GC

Cuts at:      0     963    1176 
   Size:        963     213
 
NruI TCG'CGA

Cuts at:      0     797    1176 
   Size:        797     379
 
PasI CC'CwG_GG

Cuts at:      0    1118    1176 
   Size:       1118      58
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1042    1176 
   Size:       1042     134
 
PfoI T'CCnGG_A

Cuts at:      0     743    1176 
   Size:        743     433
 
PshAI GACnn'nnGTC

Cuts at:      0     752    1176 
   Size:        752     424
 
PspOMI G'GGCC_C

Cuts at:      0     282     376     428     501     539     621    1176 
   Size:        282      94      52      73      38      82     555

  Fragments arranged by size: 

                 555     282      94      82      73      52      38
 
PspXI vC'TCGA_Gb

Cuts at:      0     660    1008    1176 
   Size:        660     348     168

  Fragments arranged by size: 

                 660     348     168
 
PstI C_TGCA'G

Cuts at:      0    1111    1176 
   Size:       1111      65
 
SacII CC_GC'GG

Cuts at:      0     306     336     355     628    1176 
   Size:        306      30      19     273     548

  Fragments arranged by size: 

                 548     306     273      30      19
 
SfcI C'TryA_G

Cuts at:      0     768     954    1107    1176 
   Size:        768     186     153      69

  Fragments arranged by size: 

                 768     186     153      69
 
SfoI GGC'GCC

Cuts at:      0     873    1176 
   Size:        873     303
 
SmaI CCC'GGG

Cuts at:      0      20     370     685    1176 
   Size:         20     350     315     491

  Fragments arranged by size: 

                 491     350     315      20
 
SmlI C'TyrA_G

Cuts at:      0     660     759    1008    1176 
   Size:        660      99     249     168

  Fragments arranged by size: 

                 660     249     168      99
 
StyI C'CwwG_G

Cuts at:      0     185    1058    1176 
   Size:        185     873     118

  Fragments arranged by size: 

                 873     185     118
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     140     730     791    1176 
   Size:        140     590      61     385

  Fragments arranged by size: 

                 590     385     140      61
 
TatI w'GTAC_w

Cuts at:      0     710    1176 
   Size:        710     466
 
XhoI C'TCGA_G

Cuts at:      0     660    1008    1176 
   Size:        660     348     168

  Fragments arranged by size: 

                 660     348     168
 
XmaI C'CCGG_G

Cuts at:      0      18     368     683    1176 
   Size:         18     350     315     493

  Fragments arranged by size: 

                 493     350     315      18
 
XmnI GAAnn'nnTTC

Cuts at:      0      28    1176 
   Size:         28    1148
 

 Enzymes that do cut:

     AleI     AloI     ApaI     AvaI     BanI    BanII     BbeI     BcgI
     BglI Bme1580I     BplI     BpmI    BpuEI    BsaHI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrFI
    BstXI     BtgI   DraIII     EaeI     EagI     EciI  Eco57MI EcoO109I
    HaeII    Hin4I     KasI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NotI     NruI     PasI    PflMI     PfoI    PshAI   PspOMI
    PspXI     PstI    SacII     SfcI     SfoI     SmaI     SmlI     StyI
    TaqII     TatI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaWI     BsgI    BsiWI    BspEI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EarI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     NdeI     NheI     NsiI     NspI     PacI     PciI
     PmeI     PmlI     PpiI    PpuMI     PsiI     PsrI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     ZraI
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