Restriction map for pF1KB0913
##################################
# NO CUT ENZYMES (  171 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AluI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaII       AviII       AvrII       BalI        BamHI       
BanII       BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BseAI       BsgI        BsiEI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr13I      ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
Ksp632I     KspI        MaeI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MspA1I      MunI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tru9I       Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AfaI          264  GTAC                 GT^AC
AlwI          198  GATCC                GGATC(4/5)
AspHI         223  GAGCAC               G[AT]GC[AT]^C
AvaI          164  CCCGGG               C^[CT]CG[GA]G
BanI           43  GGCGCC               G^G[CT][GA]CC
BbeI           43  GGCGCC               GGCGC^C
BbsI          181  GTCTTC               GAAGAC(2/6)
BbvI           66  GCTGC                GCAGC(8/12)
BmyI          223  GAGCAC               G[AGT]GC[ACT]^C
BpmI          187  CTGGAG               CTGGAG(16/14)
BpuAI         181  GTCTTC               GAAGAC(2/6)
BsaWI          56  ACCGGA               [AT]^CCGG[AT]
BseRI         157  GAGGAG               GAGGAG(10/8)
BsiHKAI       223  GAGCAC               G[AT]GC[AT]^C
BsmBI         109  CGTCTC               CGTCTC(1/5)
BsmFI          71  GGGAC                GGGAC(10/14)
BsoBI         164  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      223  GAGCAC               G[AGT]GC[ACT]^C
BsrFI          96  GCCGGC               [GA]^CCGG[CT]
BstF5I        255  GGATG                GGATG(2/0)
BstXI         251  CCATGGATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I         96  GCCGGC               [GA]^CCGG[CT]
DpnII         198  GATC                 ^GATC
DsaI          251  CCATGG               C^C[GA][CT]GG
EaeI          248  CGGCCA               [CT]^GGCC[GA]
FauI           69  GCGGG                CCCGC(4/6)
Fnu4HI         66  GCTGC                GC^[ACGT]GC
FokI          255  GGATG                GGATG(9/13)
HaeIII        249  GGCC                 GG^CC
HphI          210  GGTGA                GGTGA(8/7)
ItaI           66  GCTGC                GC^[ACGT]GC
KasI           43  GGCGCC               G^GCGCC
MboI          198  GATC                 ^GATC
MboII         182  TCTTC                GAAGA(8/7)
NaeI           96  GCCGGC               GCC^GGC
NarI           43  GGCGCC               GG^CGCC
NcoI          251  CCATGG               C^CATGG
NdeII         198  GATC                 ^GATC
NgoMIV         96  GCCGGC               G^CCGGC
NlaIII        252  CATG                 CATG^
NlaIV          43  GGCGCC               GG[ACGT]^[ACGT]CC
PleI          170  GAGTC                GAGTC(4/5)
RsaI          264  GTAC                 GT^AC
Sau3AI        198  GATC                 ^GATC
SfoI           43  GGCGCC               GGC^GCC
SmaI          164  CCCGGG               CCC^GGG
TfiI          102  GATTC                G^A[AT]TC
TseI           66  GCTGC                G^C[AT]GC
XmaCI         164  CCCGGG               C^CCGGG
XmaI          164  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccII         207  CGCG                 CG^CG
              267  CGCG                
AcyI           43  GGCGCC               G[GA]^CG[CT]C
              127  GGCGTC              
BcnI          164  CCCGG                CC^[GC]GG
              165  CCGGG               
BsaHI          43  GGCGCC               G[GA]^CG[CT]C
              127  GGCGTC              
BsaI          160  GAGACC               GGTCTC(1/5)
              145  GGTCTC              
BstNI         186  CCTGG                CC^[AT]GG
              279  CCAGG               
BstUI         207  CGCG                 CG^CG
              267  CGCG                
Cac8I          96  GCCGGC               GC[ACGT]^[ACGT]GC
              225  GCACGC              
EcoRII        186  CCTGG                ^CC[AT]GG
              279  CCAGG               
EcoT14I        81  CCAAGG               C^C[AT][AT]GG
              251  CCATGG              
HaeII          43  GGCGCC               [GA]GCGC^[CT]
               50  AGCGCC              
HgaI          205  GACGC                GACGC(5/10)
              128  GCGTC               
Hin1I          43  GGCGCC               G[GA]^CG[CT]C
              127  GGCGTC              
HinfI         102  GATTC                G^A[ACGT]TC
              170  GAGTC               
MaeII         120  ACGT                 A^CGT
              194  ACGT                
MslI          246  CACGGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              252  CATGGATGTG          
MvaI          186  CCTGG                CC^[AT]GG
              279  CCAGG               
MvnI          207  CGCG                 CG^CG
              267  CGCG                
NciI          164  CCCGG                CC^[GC]GG
              165  CCGGG               
StyI           81  CCAAGG               C^C[AT][AT]GG
              251  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AciI        BsaJI       BsiYI       BslI        BsmAI       
BssKI       CfoI        HapII       HhaI        HinP1I      
HpaII       MaeIII      MnlI        MspI        MwoI        
ScrFI       Tsp45I      
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