Restriction map for pF1KB8471
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AgeI        
AhdI        AlwI        Aor51HI     AscI        AseI        
AsnI        AspEI       AspI        AviII       AvrII       
BamHI       BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BsaBI       BsaWI       BseAI       BsiWI       BspDI       
BspEI       BssHII      BssSI       Bst1107I    BstBI       
BstYI       BstZ17I     ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KspI        MaeI        MamI        
MfeI        MflI        MluI        MroI        MunI        
NdeI        NheI        NotI        NsiI        NspV        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        ScaI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
SphI        SspI        StuI        SwaI        Tth111I     
Van91I      XbaI        XhoII       


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       1768  GGTACC               G^GTACC
Alw44I       2580  GTGCAC               G^TGCAC
ApaLI        2580  GTGCAC               G^TGCAC
Asp700I      1143  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1768  GGTACC               G^GTACC
BbeI          247  GGCGCC               GGCGC^C
BlpI         1038  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1038  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1071  CACGTA               [CT]AC^GT[GA]
BsiEI         858  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1304  TGTACA               T^GTACA
BspHI        2552  TCATGA               T^CATGA
BsrGI        1304  TGTACA               T^GTACA
BstEII       2386  GGTAACC              G^GT[ACGT]ACC
BstPI        2386  GGTAACC              G^GT[ACGT]ACC
CelII        1038  GCTGAGC              GC^T[ACGT]AGC
EagI          858  CGGCCG               C^GGCCG
EclXI         858  CGGCCG               C^GGCCG
Eco52I        858  CGGCCG               C^GGCCG
EcoNI        2166  CCTCTACGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2386  GGTAACC              G^GT[ACGT]ACC
HgaI         2066  GCGTC                GACGC(5/10)
HincII       2146  GTCAAC               GT[CT]^[GA]AC
HindII       2146  GTCAAC               GT[CT]^[GA]AC
KasI          247  GGCGCC               G^GCGCC
KpnI         1768  GGTACC               GGTAC^C
MseI         1727  TTAA                 T^TAA
NarI          247  GGCGCC               GG^CGCC
NruI         2308  TCGCGA               TCG^CGA
PaeR7I       2317  CTCGAG               C^TCGAG
RcaI         2552  TCATGA               T^CATGA
SacI         1041  GAGCTC               GAGCT^C
SbfI         1116  CCTGCAGG             CCTGCA^GG
SfiI         1252  GGCCCTAAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          247  GGCGCC               GGC^GCC
Sse8387I     1116  CCTGCAGG             CCTGCA^GG
SspBI        1304  TGTACA               T^GTACA
Tru9I        1727  TTAA                 T^TAA
XcmI          266  CCAATGGGGACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2317  CTCGAG               C^TCGAG
XmnI         1143  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AatII        2056  GACGTC               GACGT^C
             2626  GACGTC              
AcsI         1003  AAATTC               [GA]^AATT[CT]
             1846  GAATTT              
AflIII       1313  ACATGT               A^C[GA][CT]GT
             1751  ACATGT              
AlwNI        2121  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2656  CAGAGACTG           
AocI         1255  CCTAAGG              CC^T[ACGT]AGG
             1327  CCTGAGG             
ApoI         1003  AAATTC               [GA]^AATT[CT]
             1846  GAATTT              
BbsI         1732  GAAGAC               GAAGAC(2/6)
             2548  GTCTTC              
BglI         1253  GCCCTAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1803  GCCCTCCCGGC         
BpuAI        1732  GAAGAC               GAAGAC(2/6)
             2548  GTCTTC              
BsaI          367  GAGACC               GGTCTC(1/5)
              463  GAGACC              
BsmBI        2628  CGTCTC               CGTCTC(1/5)
              995  GAGACG              
BspLU11I     1313  ACATGT               A^CATGT
             1751  ACATGT              
BspMI        1119  GCAGGT               ACCTGC(4/8)
             1664  GCAGGT              
BsrBI          52  GAGCGG               CCGCTC(-3/-3)
             1429  GAGCGG              
Bsu36I       1255  CCTAAGG              CC^T[ACGT]AGG
             1327  CCTGAGG             
Eco81I       1255  CCTAAGG              CC^T[ACGT]AGG
             1327  CCTGAGG             
HaeII         110  AGCGCC               [GA]GCGC^[CT]
              247  GGCGCC              
NaeI          503  GCCGGC               GCC^GGC
             2183  GCCGGC              
NcoI          230  CCATGG               C^CATGG
             2279  CCATGG              
NgoMIV        503  GCCGGC               G^CCGGC
             2183  GCCGGC              
NspI         1313  ACATGT               [GA]CATG^[CT]
             1751  ACATGT              
SapI         1374  GAAGAGC              GCTCTTC(1/4)
             2187  GCTCTTC             
SmlI          384  CTCAAG               C^T[CT][GA]AG
             2317  CTCGAG              
Tsp509I      1004  AATT                 ^AATT
             1847  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
ApaI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BsrDI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StyI        TaqI        TfiI        TseI        
Tsp45I      TspRI       TthHB8I     XmaCI       XmaI        
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