Restriction map for pF1KB9995
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AlwNI       Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AvaI        
AvaII       AviII       AvrII       BamHI       BbrPI       
BciVI       BclI        BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     CelII       
ClaI        CpoI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO109I    EcoO65I     EcoRI       EcoRV       FbaI        
FseI        FspI        HpaI        KpnI        MamI        
MfeI        MroI        MunI        NcoI        NdeI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AocI          872  CCTTAGG              CC^T[ACGT]AGG
Asp700I       369  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1209  GTGCTC               G[AT]GC[AT]^C
AspI         1654  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          688  TGGCCA               TGG^CCA
BbeI          727  GGCGCC               GGCGC^C
BbsI         1549  GAAGAC               GAAGAC(2/6)
BbvCI        1495  CCTCAGC              CCTCAGC(-5/-2)
BcgI           79  CGAGCCTGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          527  CTAG                 C^TAG
BglI          202  GCCCGGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1073  AGATCT               A^GATCT
BpuAI        1549  GAAGAC               GAAGAC(2/6)
BsiHKAI      1209  GTGCTC               G[AT]GC[AT]^C
BsmBI         149  GAGACG               CGTCTC(1/5)
BspMI         287  ACCTGC               ACCTGC(4/8)
BsrBI         171  GAGCGG               CCGCTC(-3/-3)
BssSI         991  CTCGTG               CACGAG(-5/-1)
BstXI        1604  CCAGAATGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1073  AGATCT               [GA]^GATC[CT]
Bsu36I        872  CCTTAGG              CC^T[ACGT]AGG
DraI         1357  TTTAAA               TTT^AAA
DsaI          190  CCGCGG               C^C[GA][CT]GG
EaeI          688  TGGCCA               [CT]^GGCC[GA]
Eco81I        872  CCTTAGG              CC^T[ACGT]AGG
EcoNI        1575  CCTCTCGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII         727  GGCGCC               [GA]GCGC^[CT]
HincII        259  GTCAAC               GT[CT]^[GA]AC
HindII        259  GTCAAC               GT[CT]^[GA]AC
HindIII      1533  AAGCTT               A^AGCTT
KasI          727  GGCGCC               G^GCGCC
KspI          190  CCGCGG               CCGC^GG
MaeI          527  CTAG                 C^TAG
MflI         1073  AGATCT               [GA]^GATC[CT]
MluI          266  ACGCGT               A^CGCGT
MluNI         688  TGGCCA               TGG^CCA
MscI          688  TGGCCA               TGG^CCA
NarI          727  GGCGCC               GG^CGCC
PflFI        1654  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1641  CCAACCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII         190  CCGCGG               CCGC^GG
SapI          805  GCTCTTC              GCTCTTC(1/4)
SfoI          727  GGCGCC               GGC^GCC
SmlI          853  CTCAAG               C^T[CT][GA]AG
SphI         1263  GCATGC               GCATG^C
StuI         1325  AGGCCT               AGG^CCT
Tth111I      1654  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1641  CCAACCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        1073  AGATCT               [GA]^GATC[CT]
XmnI          369  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcsI          462  AAATTT               [GA]^AATT[CT]
              473  AAATTT              
ApoI          462  AAATTT               [GA]^AATT[CT]
              473  AAATTT              
BanI           86  GGTGCC               G^G[CT][GA]CC
              727  GGCGCC              
BanII          12  GGGCTC               G[GA]GC[CT]^C
              696  GGGCTC              
BspLU11I      608  ACATGT               A^CATGT
              740  ACATGT              
BsrDI         484  CATTGC               GCAATG(2/0)
              819  CATTGC              
BsrFI         162  GCCGGC               [GA]^CCGG[CT]
              294  GCCGGC              
Cfr10I        162  GCCGGC               [GA]^CCGG[CT]
              294  GCCGGC              
EcoT14I      1646  CCTTGG               C^C[AT][AT]GG
             1740  CCTTGG              
HgaI          151  GACGC                GACGC(5/10)
             1340  GACGC               
MaeIII        655  GTCAC                ^GT[ACGT]AC
             1168  GTGAC               
NaeI          162  GCCGGC               GCC^GGC
              294  GCCGGC              
NgoMIV        162  GCCGGC               G^CCGGC
              294  GCCGGC              
PleI         1196  GAGTC                GAGTC(4/5)
             1569  GAGTC               
PstI          237  CTGCAG               CTGCA^G
              866  CTGCAG              
PvuII         240  CAGCTG               CAG^CTG
             1004  CAGCTG              
SfcI          237  CTGCAG               C^T[GA][CT]AG
              866  CTGCAG              
StyI         1646  CCTTGG               C^C[AT][AT]GG
             1740  CCTTGG              
TaqI          377  TCGA                 T^CGA
             1579  TCGA                
Tsp45I        655  GTCAC                ^GT[GC]AC
             1168  GTGAC               
TthHB8I       377  TCGA                 T^CGA
             1579  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        BbvI        BcnI        BmyI        BpmI        
BsaHI       BsaJI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EarI        EcoRII      EcoT22I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TfiI        Tru9I       TseI        Tsp509I     TspRI       
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