Restriction map for pF1KB0973
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvrII       BalI        BamHI       BbrPI       BbsI        
BbvCI       BciVI       BclI        BfrI        BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaBI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HincII      
HindII      HindIII     HinfI       HpaI        KpnI        
Ksp632I     KspI        MamI        MboII       MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        NaeI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tru9I       
Tsp509I     Tth111I     Van91I      XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII          45  CGCG                 CG^CG
AcyI          545  GGCGCC               G[GA]^CG[CT]C
AlwI          483  GATCC                GGATC(4/5)
AvaI          370  CCCGGG               C^[CT]CG[GA]G
AviII          53  TGCGCA               TGC^GCA
BanI          545  GGCGCC               G^G[CT][GA]CC
BbeI          545  GGCGCC               GGCGC^C
BcgI           34  CGAGGCCGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          496  CTAG                 C^TAG
BglI          574  GCCTTCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         443  AGATCT               A^GATCT
BsaAI         595  TACGTG               [CT]AC^GT[GA]
BsaHI         545  GGCGCC               G[GA]^CG[CT]C
BseRI          91  GAGGAG               GAGGAG(10/8)
BsmBI         109  GAGACG               CGTCTC(1/5)
BsmFI         402  GTCCC                GGGAC(10/14)
BsoBI         370  CCCGGG               C^[CT]CG[GA]G
BspMI         464  ACCTGC               ACCTGC(4/8)
BstEII         99  GGTGACC              G^GT[ACGT]ACC
BstPI          99  GGTGACC              G^GT[ACGT]ACC
BstUI          45  CGCG                 CG^CG
BstYI         443  AGATCT               [GA]^GATC[CT]
DrdI          393  GACCCTGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I        99  GGTGACC              G^GT[ACGT]ACC
EcoT14I       228  CCTTGG               C^C[AT][AT]GG
FauI          340  GCGGG                CCCGC(4/6)
FspI           53  TGCGCA               TGC^GCA
HaeII         545  GGCGCC               [GA]GCGC^[CT]
HgaI          210  GCGTC                GACGC(5/10)
Hin1I         545  GGCGCC               G[GA]^CG[CT]C
KasI          545  GGCGCC               G^GCGCC
MaeI          496  CTAG                 C^TAG
MflI          443  AGATCT               [GA]^GATC[CT]
MvnI           45  CGCG                 CG^CG
NarI          545  GGCGCC               GG^CGCC
NdeI          536  CATATG               CA^TATG
NlaIV         545  GGCGCC               GG[ACGT]^[ACGT]CC
NspI           56  GCATGC               [GA]CATG^[CT]
SfaNI         514  GATGC                GCATC(5/9)
SfiI          573  GGCCTTCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          545  GGCGCC               GGC^GCC
SmaI          370  CCCGGG               CCC^GGG
SmlI          568  CTCAAG               C^T[CT][GA]AG
SphI           56  GCATGC               GCATG^C
StuI          572  AGGCCT               AGG^CCT
StyI          228  CCTTGG               C^C[AT][AT]GG
TaqI           33  TCGA                 T^CGA
TthHB8I        33  TCGA                 T^CGA
XhoII         443  AGATCT               [GA]^GATC[CT]
XmaCI         370  CCCGGG               C^CCGGG
XmaI          370  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AfaI          189  GTAC                 GT^AC
              594  GTAC                
BanII         123  GGGCTC               G[GA]GC[CT]^C
              232  GGGCTC              
BsrI           28  CCAGT                ACTGG(1/-1)
              192  CCAGT               
DraII         293  AGGACCT              [GA]G^G[ACGT]CC[CT]
              491  AGGTCCT             
DsaI           14  CCGTGG               C^C[GA][CT]GG
              104  CCGTGG              
EcoO109I      293  AGGACCT              [GA]G^G[ACGT]CC[CT]
              491  AGGTCCT             
HapII         371  CCGG                 C^CGG
              558  CCGG                
HpaII         371  CCGG                 C^CGG
              558  CCGG                
MaeII         112  ACGT                 A^CGT
              596  ACGT                
MaeIII        100  GTGAC                ^GT[ACGT]AC
              212  GTCAC               
MspA1I         39  CCGCTG               C[AC]G^C[GT]G
              300  CCGCTG              
MspI          371  CCGG                 C^CGG
              558  CCGG                
PpuMI         293  AGGACCT              [GA]G^G[AT]CC[CT]
              491  AGGTCCT             
RsaI          189  GTAC                 GT^AC
              594  GTAC                
Tsp45I        100  GTGAC                ^GT[GC]AC
              212  GTCAC               
TspRI         365  CACTG                CA[GC]TG[ACGT][ACGT]^
              552  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BcnI        
BmyI        BsaI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HphI        ItaI        MboI        MnlI        
MvaI        MwoI        NciI        NdeII       NlaIII      
Sau3AI      Sau96I      ScrFI       TseI        
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