Restriction map for pF1KB0973
 (Linear) (Six-base) MAPSORT of: KIBB0973.seq  Check: 9709  from: 1  to: 630



KIBB0973.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     630 
   Size:        400     230
 
AvaI C'yCGr_G

Cuts at:      0     370     630 
   Size:        370     260
 
BanI G'GyrC_C

Cuts at:      0     545     630 
   Size:        545      85
 
BanII G_rGCy'C

Cuts at:      0     127     236     630 
   Size:        127     109     394

  Fragments arranged by size: 

                 394     127     109
 
BbeI G_GCGC'C

Cuts at:      0     549     630 
   Size:        549      81
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      23      57     630 
   Size:         23      34     573

  Fragments arranged by size: 

                 573      34      23
 
BglI GCCn_nnn'nGGC

Cuts at:      0     580     630 
   Size:        580      50
 
BglII A'GATC_T

Cuts at:      0     443     630 
   Size:        443     187
 
Bme1580I G_kGCm'C

Cuts at:      0     558     630 
   Size:        558      72
 
BmgBI CAC'GTC

Cuts at:      0     113     630 
   Size:        113     517
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      22     630 
   Size:         22     608
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     553     630 
   Size:        553      77
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     170     385     431     630 
   Size:        170     215      46     199

  Fragments arranged by size: 

                 215     199     170      46
 
BsaAI yAC'GTr

Cuts at:      0     597     630 
   Size:        597      33
 
BsaHI Gr'CG_yC

Cuts at:      0     546     630 
   Size:        546      84
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     430     460     630 
   Size:        430      30     170

  Fragments arranged by size: 

                 430     170      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     106     630 
   Size:        106     524
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     103     630 
   Size:        103     527
 
Bsp1286I G_dGCh'C

Cuts at:      0     127     236     558     630 
   Size:        127     109     322      72

  Fragments arranged by size: 

                 322     127     109      72
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     473     630 
   Size:        473     157
 
BstEII G'GTnAC_C

Cuts at:      0      99     630 
   Size:         99     531
 
BstYI r'GATC_y

Cuts at:      0     443     630 
   Size:        443     187
 
BtgI C'CryG_G

Cuts at:      0      14     104     630 
   Size:         14      90     526

  Fragments arranged by size: 

                 526      90      14
 
BtsI GCAGTG_nn'

Cuts at:      0     364     630 
   Size:        364     266
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     399     630 
   Size:        399     231
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     630 
   Size:        400     230
 
EcoO109I rG'GnC_Cy

Cuts at:      0     294     492     630 
   Size:        294     198     138

  Fragments arranged by size: 

                 294     198     138
 
FspI TGC'GCA

Cuts at:      0      55     630 
   Size:         55     575
 
HaeII r_GCGC'y

Cuts at:      0     549     630 
   Size:        549      81
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     428     430     460     462     630 
   Size:        428       2      30       2     168

  Fragments arranged by size: 

                 428     168      30       2       2
 
KasI G'GCGC_C

Cuts at:      0     545     630 
   Size:        545      85
 
MspA1I CmG'CkG

Cuts at:      0      41     302     630 
   Size:         41     261     328

  Fragments arranged by size: 

                 328     261      41
 
NarI GG'CG_CC

Cuts at:      0     546     630 
   Size:        546      84
 
NdeI CA'TA_TG

Cuts at:      0     537     630 
   Size:        537      93
 
NspI r_CATG'y

Cuts at:      0      60     630 
   Size:         60     570
 
PfoI T'CCnGG_A

Cuts at:      0     167     630 
   Size:        167     463
 
PpuMI rG'GwC_Cy

Cuts at:      0     294     492     630 
   Size:        294     198     138

  Fragments arranged by size: 

                 294     198     138
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     580     630 
   Size:        580      50
 
SfoI GGC'GCC

Cuts at:      0     547     630 
   Size:        547      83
 
SmaI CCC'GGG

Cuts at:      0     372     630 
   Size:        372     258
 
SmlI C'TyrA_G

Cuts at:      0     568     630 
   Size:        568      62
 
SphI G_CATG'C

Cuts at:      0      60     630 
   Size:         60     570
 
SrfI GCCC'GGGC

Cuts at:      0     372     630 
   Size:        372     258
 
StuI AGG'CCT

Cuts at:      0     574     630 
   Size:        574      56
 
StyI C'CwwG_G

Cuts at:      0     228     630 
   Size:        228     402
 
XmaI C'CCGG_G

Cuts at:      0     370     630 
   Size:        370     260
 

 Enzymes that do cut:

     AcuI     AvaI     BanI    BanII     BbeI     BcgI     BglI    BglII
 Bme1580I    BmgBI     BmrI    BpuEI     BsaI    BsaAI    BsaHI    BsaXI
    BseRI    BsmBI Bsp1286I    BspMI   BstEII    BstYI     BtgI     BtsI
     DrdI  Eco57MI EcoO109I     FspI    HaeII    Hin4I     KasI   MspA1I
     NarI     NdeI     NspI     PfoI    PpuMI     SfiI     SfoI     SmaI
     SmlI     SphI     SrfI     StuI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI
    BbvCI    BciVI     BclI    BfrBI     BlpI     BmtI     BplI     BpmI
   Bpu10I    BsaBI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SgfI
    SgrAI    SnaBI     SpeI     SspI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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