(Linear) (Six-base) MAPSORT of: KIBB0970.seq  Check: 4404  from: 1  to: 1293



KIBB0970.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AclI AA'CG_TT

Cuts at:      0    1245    1293 
   Size:       1245      48
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     740    1293 
   Size:        740     553
 
AhdI GACnn_n'nnGTC

Cuts at:      0     123    1293 
   Size:        123    1170
 
ApoI r'AATT_y

Cuts at:      0     384    1293 
   Size:        384     909
 
AseI AT'TA_AT

Cuts at:      0     191    1293 
   Size:        191    1102
 
BamHI G'GATC_C

Cuts at:      0     529    1293 
   Size:        529     764
 
BanI G'GyrC_C

Cuts at:      0     924    1293 
   Size:        924     369
 
BanII G_rGCy'C

Cuts at:      0     161    1293 
   Size:        161    1132
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     188    1034    1293 
   Size:        188     846     259

  Fragments arranged by size: 

                 846     259     188
 
BclI T'GATC_A

Cuts at:      0     633    1268    1293 
   Size:        633     635      25

  Fragments arranged by size: 

                 635     633      25
 
Bme1580I G_kGCm'C

Cuts at:      0     579    1293 
   Size:        579     714
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     565    1293 
   Size:        565     728
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161    1236    1293 
   Size:        161    1075      57

  Fragments arranged by size: 

                1075     161      57
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     786     816    1293 
   Size:        786      30     477

  Fragments arranged by size: 

                 786     477      30
 
BseYI C'CCAG_C

Cuts at:      0     578     678     779    1293 
   Size:        578     100     101     514

  Fragments arranged by size: 

                 578     514     101     100
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1001    1293 
   Size:       1001     292
 
Bsp1286I G_dGCh'C

Cuts at:      0     161     579    1293 
   Size:        161     418     714

  Fragments arranged by size: 

                 714     418     161
 
BsrDI GCAATG_nn'

Cuts at:      0     159     893    1293 
   Size:        159     734     400

  Fragments arranged by size: 

                 734     400     159
 
BstYI r'GATC_y

Cuts at:      0     529    1277    1293 
   Size:        529     748      16

  Fragments arranged by size: 

                 748     529      16
 
BtsI GCAGTG_nn'

Cuts at:      0    1116    1293 
   Size:       1116     177
 
EaeI y'GGCC_r

Cuts at:      0     429     791    1293 
   Size:        429     362     502

  Fragments arranged by size: 

                 502     429     362
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     565     740    1293 
   Size:        565     175     553

  Fragments arranged by size: 

                 565     553     175
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1163    1293 
   Size:       1163     130
 
EcoRV GAT'ATC

Cuts at:      0     495    1293 
   Size:        495     798
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     109     141     355     387    1293 
   Size:        109      32     214      32     906

  Fragments arranged by size: 

                 906     214     109      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1001    1293 
   Size:       1001     292
 
MscI TGG'CCA

Cuts at:      0     431     793    1293 
   Size:        431     362     500

  Fragments arranged by size: 

                 500     431     362
 
MspA1I CmG'CkG

Cuts at:      0     789    1293 
   Size:        789     504
 
NspI r_CATG'y

Cuts at:      0     236    1293 
   Size:        236    1057
 
PasI CC'CwG_GG

Cuts at:      0    1104    1293 
   Size:       1104     189
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     888    1293 
   Size:        888     405
 
PpuMI rG'GwC_Cy

Cuts at:      0    1163    1293 
   Size:       1163     130
 
PsiI TTA'TAA

Cuts at:      0     593    1293 
   Size:        593     700
 
PvuII CAG'CTG

Cuts at:      0     789    1293 
   Size:        789     504
 
SanDI GG'GwC_CC

Cuts at:      0    1163    1293 
   Size:       1163     130
 
SfcI C'TryA_G

Cuts at:      0     851    1293 
   Size:        851     442
 
SmlI C'TyrA_G

Cuts at:      0     176    1251    1293 
   Size:        176    1075      42

  Fragments arranged by size: 

                1075     176      42
 
StuI AGG'CCT

Cuts at:      0    1199    1293 
   Size:       1199      94
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     786     818    1293 
   Size:        786      32     475

  Fragments arranged by size: 

                 786     475      32
 
XbaI T'CTAG_A

Cuts at:      0      99    1293 
   Size:         99    1194
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1145    1293 
   Size:       1145     148
 

 Enzymes that do cut:

     AclI     AcuI     AhdI     ApoI     AseI    BamHI     BanI    BanII
     BbsI     BclI Bme1580I     BpmI    BpuEI    BsaXI    BseYI     BsgI
 Bsp1286I    BsrDI    BstYI     BtsI     EaeI  Eco57MI EcoO109I    EcoRV
    Hin4I     MmeI     MscI   MspA1I     NspI     PasI    PflMI    PpuMI
     PsiI    PvuII    SanDI     SfcI     SmlI     StuI     TstI     XbaI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI     BbeI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI     PfoI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StyI     SwaI    TaqII     TatI  Tth111I     XhoI
     XmaI     XmnI     ZraI