Restriction map for pF1KB0967
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AviII       BamHI       BbrPI       BbsI        BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BpuAI       BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstYI       BstZ17I     Cfr10I      
ClaI        CpoI        DpnII       DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
Ksp632I     KspI        MamI        MboI        MboII       
MfeI        MflI        MluI        MroI        MseI        
MslI        MunI        NaeI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      ScaI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        Tru9I       Tsp509I     TthHB8I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AocI           17  CCTTAGG              CC^T[ACGT]AGG
AvrII         207  CCTAGG               C^CTAGG
BalI          137  TGGCCA               TGG^CCA
BcnI          198  CCCGG                CC^[GC]GG
BlnI          207  CCTAGG               C^CTAGG
BlpI          283  GCTGAGC              GC^T[ACGT]AGC
BpmI           90  CTCCAG               CTGGAG(16/14)
Bpu1102I      283  GCTGAGC              GC^T[ACGT]AGC
BsmAI         452  GAGAC                GTCTC(1/5)
BsmFI         427  GTCCC                GGGAC(10/14)
BsrDI         396  GCAATG               GCAATG(2/0)
BstXI         259  CCAGGTCGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I         17  CCTTAGG              CC^T[ACGT]AGG
CelII         283  GCTGAGC              GC^T[ACGT]AGC
EaeI          137  TGGCCA               [CT]^GGCC[GA]
Eco81I         17  CCTTAGG              CC^T[ACGT]AGG
EcoNI         379  CCTGGGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII         199  CCGG                 C^CGG
HgaI          409  GCGTC                GACGC(5/10)
HinfI         178  GACTC                G^A[ACGT]TC
HpaII         199  CCGG                 C^CGG
MaeII         350  ACGT                 A^CGT
MluNI         137  TGGCCA               TGG^CCA
MscI          137  TGGCCA               TGG^CCA
MspA1I        584  CAGCTG               C[AC]G^C[GT]G
MspI          199  CCGG                 C^CGG
NciI          198  CCCGG                CC^[GC]GG
NlaIII        604  CATG                 CATG^
PleI          178  GACTC                GAGTC(4/5)
PpuMI         425  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         584  CAGCTG               CAG^CTG
SbfI          366  CCTGCAGG             CCTGCA^GG
SexAI         473  ACCTGGT              A^CC[AT]GGT
SfaNI          30  GATGC                GCATC(5/9)
SmlI          310  CTTGAG               C^T[CT][GA]AG
Sse8387I      366  CCTGCAGG             CCTGCA^GG
XcmI          348  CCACGTCGCGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AspI          346  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              472  GACCTGGTC           
BbeI          161  GGCGCC               GGCGC^C
              236  GGCGCC              
BfaI          148  CTAG                 C^TAG
              208  CTAG                
BsgI           12  CTGCAC               GTGCAG(16/14)
              538  CTGCAC              
BspMI         536  ACCTGC               ACCTGC(4/8)
              423  GCAGGT              
BsrI          142  ACTGG                ACTGG(1/-1)
              153  ACTGG               
EcoT14I        53  CCTTGG               C^C[AT][AT]GG
              207  CCTAGG              
FauI          203  CCCGC                CCCGC(4/6)
              435  GCGGG               
HphI          101  TCACC                GGTGA(8/7)
              608  TCACC               
KasI          161  GGCGCC               G^GCGCC
              236  GGCGCC              
MaeI          148  CTAG                 C^TAG
              208  CTAG                
NarI          161  GGCGCC               GG^CGCC
              236  GGCGCC              
PflFI         346  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              472  GACCTGGTC           
PstI          271  CTGCAG               CTGCA^G
              367  CTGCAG              
SfcI          271  CTGCAG               C^T[GA][CT]AG
              367  CTGCAG              
SfoI          161  GGCGCC               GGC^GCC
              236  GGCGCC              
StyI           53  CCTTGG               C^C[AT][AT]GG
              207  CCTAGG              
Tth111I       346  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              472  GACCTGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AvaI        
AvaII       BanI        BanII       BbvCI       BbvI        
BmyI        BsaHI       BsaJI       BsiYI       BslI        
BsoBI       Bsp1286I    BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeII       HaeIII      HhaI        Hin1I       HinP1I      
ItaI        MaeIII      MnlI        MvaI        MvnI        
MwoI        NlaIV       Sau96I      ScrFI       TseI        
Tsp45I      TspRI       
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