Restriction map for pF1KB0967
 (Linear) (Six-base) MAPSORT of: KIBB0967.seq  Check: 196  from: 1  to: 618



KIBB0967.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     563     618 
   Size:        563      55
 
AvaI C'yCGr_G

Cuts at:      0      25      60     278     618 
   Size:         25      35     218     340

  Fragments arranged by size: 

                 340     218      35      25
 
AvrII C'CTAG_G

Cuts at:      0     207     618 
   Size:        207     411
 
BanI G'GyrC_C

Cuts at:      0     161     211     236     618 
   Size:        161      50      25     382

  Fragments arranged by size: 

                 382     161      50      25
 
BanII G_rGCy'C

Cuts at:      0      26      61      91     618 
   Size:         26      35      30     527

  Fragments arranged by size: 

                 527      35      30      26
 
BbeI G_GCGC'C

Cuts at:      0     165     240     618 
   Size:        165      75     378

  Fragments arranged by size: 

                 378     165      75
 
BbvCI CC'TCA_GC

Cuts at:      0     169     440     518     618 
   Size:        169     271      78     100

  Fragments arranged by size: 

                 271     169     100      78
 
BlpI GC'TnA_GC

Cuts at:      0     284     618 
   Size:        284     334
 
BmgBI CAC'GTC

Cuts at:      0     351     618 
   Size:        351     267
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     618 
   Size:         75     543
 
Bpu10I CC'TnA_GC

Cuts at:      0     169     440     518     618 
   Size:        169     271      78     100

  Fragments arranged by size: 

                 271     169     100      78
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     618 
   Size:        331     287
 
BsaHI Gr'CG_yC

Cuts at:      0     162     237     409     618 
   Size:        162      75     172     209

  Fragments arranged by size: 

                 209     172     162      75
 
BseYI C'CCAG_C

Cuts at:      0     288     586     618 
   Size:        288     298      32

  Fragments arranged by size: 

                 298     288      32
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     618 
   Size:        523      95
 
Bsp1286I G_dGCh'C

Cuts at:      0      26      61      91     618 
   Size:         26      35      30     527

  Fragments arranged by size: 

                 527      35      30      26
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     414     545     618 
   Size:        414     131      73

  Fragments arranged by size: 

                 414     131      73
 
BsrDI GCAATG_nn'

Cuts at:      0     403     618 
   Size:        403     215
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     266     618 
   Size:        266     352
 
Bsu36I CC'TnA_GG

Cuts at:      0      18     618 
   Size:         18     600
 
BtsI GCAGTG_nn'

Cuts at:      0     519     618 
   Size:        519      99
 
EaeI y'GGCC_r

Cuts at:      0     137     618 
   Size:        137     481
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     618 
   Size:         75     543
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     383     618 
   Size:        383     235
 
EcoO109I rG'GnC_Cy

Cuts at:      0      50     426     522     597     618 
   Size:         50     376      96      75      21

  Fragments arranged by size: 

                 376      96      75      50      21
 
HaeII r_GCGC'y

Cuts at:      0     165     240     578     618 
   Size:        165      75     338      40

  Fragments arranged by size: 

                 338     165      75      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     277     309     618 
   Size:        277      32     309

  Fragments arranged by size: 

                 309     277      32
 
KasI G'GCGC_C

Cuts at:      0     161     236     618 
   Size:        161      75     382

  Fragments arranged by size: 

                 382     161      75
 
MscI TGG'CCA

Cuts at:      0     139     618 
   Size:        139     479
 
MspA1I CmG'CkG

Cuts at:      0     586     618 
   Size:        586      32
 
NarI GG'CG_CC

Cuts at:      0     162     237     618 
   Size:        162      75     381

  Fragments arranged by size: 

                 381     162      75
 
PasI CC'CwG_GG

Cuts at:      0     379     618 
   Size:        379     239
 
PpuMI rG'GwC_Cy

Cuts at:      0     426     618 
   Size:        426     192
 
PstI C_TGCA'G

Cuts at:      0     275     371     618 
   Size:        275      96     247

  Fragments arranged by size: 

                 275     247      96
 
PvuII CAG'CTG

Cuts at:      0     586     618 
   Size:        586      32
 
SbfI CC_TGCA'GG

Cuts at:      0     371     618 
   Size:        371     247
 
SexAI A'CCwGG_T

Cuts at:      0     473     618 
   Size:        473     145
 
SfcI C'TryA_G

Cuts at:      0     271     367     618 
   Size:        271      96     251

  Fragments arranged by size: 

                 271     251      96
 
SfoI GGC'GCC

Cuts at:      0     163     238     618 
   Size:        163      75     380

  Fragments arranged by size: 

                 380     163      75
 
SmlI C'TyrA_G

Cuts at:      0     310     618 
   Size:        310     308
 
StyI C'CwwG_G

Cuts at:      0      53     207     618 
   Size:         53     154     411

  Fragments arranged by size: 

                 411     154      53
 
Tth111I GACn'n_nGTC

Cuts at:      0     349     475     618 
   Size:        349     126     143

  Fragments arranged by size: 

                 349     143     126
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     355     618 
   Size:        355     263
 

 Enzymes that do cut:

     AlfI     AvaI    AvrII     BanI    BanII     BbeI    BbvCI     BlpI
    BmgBI     BpmI   Bpu10I    BpuEI    BsaHI    BseYI     BsgI Bsp1286I
    BspMI    BsrDI    BstXI   Bsu36I     BtsI     EaeI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I     KasI     MscI   MspA1I     NarI     PasI
    PpuMI     PstI    PvuII     SbfI    SexAI     SfcI     SfoI     SmlI
     StyI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     BaeI    BamHI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII Bme1580I     BmrI     BmtI
     BplI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     ScaI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI     XbaI     XhoI     XmaI     XmnI
     ZraI
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