Restriction map for pF1KB0956
 (Linear) (Six-base) MAPSORT of: KIBB0956.seq  Check: 6698  from: 1  to: 1254



KIBB0956.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     913    1254 
   Size:        436     477     341

  Fragments arranged by size: 

                 477     436     341
 
AflIII A'CryG_T

Cuts at:      0      20    1254 
   Size:         20    1234
 
AleI CACnn'nnGTG

Cuts at:      0     950    1254 
   Size:        950     304
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1022    1254 
   Size:       1022     232
 
AlwNI CAG_nnn'CTG

Cuts at:      0     258     282     382     402     886    1153    1254 
   Size:        258      24     100      20     484     267     101

  Fragments arranged by size: 

                 484     267     258     101     100      24      20
 
ApaLI G'TGCA_C

Cuts at:      0     300    1254 
   Size:        300     954
 
ApoI r'AATT_y

Cuts at:      0     634    1037    1254 
   Size:        634     403     217

  Fragments arranged by size: 

                 634     403     217
 
Bme1580I G_kGCm'C

Cuts at:      0     228     304     409     959    1254 
   Size:        228      76     105     550     295

  Fragments arranged by size: 

                 550     295     228     105      76
 
BmgBI CAC'GTC

Cuts at:      0      21    1254 
   Size:         21    1233
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     567    1254 
   Size:        567     687
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     698     832     908    1033    1254 
   Size:        698     134      76     125     221

  Fragments arranged by size: 

                 698     221     134     125      76
 
Bpu10I CC'TnA_GC

Cuts at:      0     781    1254 
   Size:        781     473
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      66     130     734     961    1254 
   Size:         66      64     604     227     293

  Fragments arranged by size: 

                 604     293     227      66      64
 
BsaHI Gr'CG_yC

Cuts at:      0     845    1254 
   Size:        845     409
 
BsaWI w'CCGG_w

Cuts at:      0     125     725    1254 
   Size:        125     600     529

  Fragments arranged by size: 

                 600     529     125
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      10      40    1254 
   Size:         10      30    1214

  Fragments arranged by size: 

                1214      30      10
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     256     480     616    1207    1210    1213    1216    1254 
   Size:        256     224     136     591       3       3       3      38

  Fragments arranged by size: 

                 591     256     224     136      38       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0    1230    1254 
   Size:       1230      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     380    1254 
   Size:        152     228     874

  Fragments arranged by size: 

                 874     228     152
 
BsiHKAI G_wGCw'C

Cuts at:      0     304    1254 
   Size:        304     950
 
BsmI GAATG_Cn'

Cuts at:      0     189     252    1254 
   Size:        189      63    1002

  Fragments arranged by size: 

                1002     189      63
 
Bsp1286I G_dGCh'C

Cuts at:      0     228     304     409     959    1254 
   Size:        228      76     105     550     295

  Fragments arranged by size: 

                 550     295     228     105      76
 
BspEI T'CCGG_A

Cuts at:      0     125    1254 
   Size:        125    1129
 
BsrDI GCAATG_nn'

Cuts at:      0     336    1254 
   Size:        336     918
 
BssHII G'CGCG_C

Cuts at:      0      53    1254 
   Size:         53    1201
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1143    1254 
   Size:       1143     111
 
BstYI r'GATC_y

Cuts at:      0     910    1097    1254 
   Size:        910     187     157

  Fragments arranged by size: 

                 910     187     157
 
Bsu36I CC'TnA_GG

Cuts at:      0     220     824    1102    1254 
   Size:        220     604     278     152

  Fragments arranged by size: 

                 604     278     220     152
 
BtgI C'CryG_G

Cuts at:      0      65    1254 
   Size:         65    1189
 
BtsI GCAGTG_nn'

Cuts at:      0     213     363    1004    1254 
   Size:        213     150     641     250

  Fragments arranged by size: 

                 641     250     213     150
 
EaeI y'GGCC_r

Cuts at:      0     521     756    1254 
   Size:        521     235     498

  Fragments arranged by size: 

                 521     498     235
 
EarI CTCTTCn'nnn_

Cuts at:      0     593     673    1172    1178    1184    1254 
   Size:        593      80     499       6       6      70

  Fragments arranged by size: 

                 593     499      80      70       6       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     698     832     908     913    1033    1254 
   Size:        436     262     134      76       5     120     221

  Fragments arranged by size: 

                 436     262     221     134     120      76       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     685    1147    1254 
   Size:        685     462     107

  Fragments arranged by size: 

                 685     462     107
 
EcoO109I rG'GnC_Cy

Cuts at:      0     399     412     678    1254 
   Size:        399      13     266     576

  Fragments arranged by size: 

                 576     399     266      13
 
FspI TGC'GCA

Cuts at:      0     310    1254 
   Size:        310     944
 
HaeII r_GCGC'y

Cuts at:      0      92    1254 
   Size:         92    1162
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      10      42     902     934    1254 
   Size:         10      32     860      32     320

  Fragments arranged by size: 

                 860     320      32      32      10
 
HindIII A'AGCT_T

Cuts at:      0     448    1254 
   Size:        448     806
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     535    1254 
   Size:        535     719
 
MscI TGG'CCA

Cuts at:      0     523     758    1254 
   Size:        523     235     496

  Fragments arranged by size: 

                 523     496     235
 
MslI CAynn'nnrTG

Cuts at:      0     505     800     950    1254 
   Size:        505     295     150     304

  Fragments arranged by size: 

                 505     304     295     150
 
MspA1I CmG'CkG

Cuts at:      0    1068    1254 
   Size:       1068     186
 
NspI r_CATG'y

Cuts at:      0    1003    1254 
   Size:       1003     251
 
PpuMI rG'GwC_Cy

Cuts at:      0     399    1254 
   Size:        399     855
 
PshAI GACnn'nnGTC

Cuts at:      0    1061    1254 
   Size:       1061     193
 
PstI C_TGCA'G

Cuts at:      0     358    1254 
   Size:        358     896
 
SexAI A'CCwGG_T

Cuts at:      0     395    1254 
   Size:        395     859
 
SfcI C'TryA_G

Cuts at:      0     354    1254 
   Size:        354     900
 
StyI C'CwwG_G

Cuts at:      0      92     759    1254 
   Size:         92     667     495

  Fragments arranged by size: 

                 667     495      92
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     305    1254 
   Size:        305     949
 
TatI w'GTAC_w

Cuts at:      0     350    1254 
   Size:        350     904
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     107     139    1254 
   Size:        107      32    1115

  Fragments arranged by size: 

                1115     107      32
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AlfI    AlwNI    ApaLI     ApoI Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BsrDI
   BssHII    BstXI    BstYI   Bsu36I     BtgI     BtsI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I  HindIII     MmeI
     MscI     MslI   MspA1I     NspI    PpuMI    PshAI     PstI    SexAI
     SfcI     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AloI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmtI     BplI
    BpuEI    BsaAI    BsaBI    BsiEI    BsiWI    BsmBI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com