Restriction map for pF1KB6633
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        AlwNI       
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AviII       
AvrII       BamHI       BbrPI       BcgI        BfrI        
BglI        BglII       BlnI        BsaWI       BseAI       
BsmBI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DrdI        Eam1105I    EarI        
Eco47III    EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HincII      HindII      
HindIII     HpaI        Ksp632I     MfeI        MluI        
MroI        MseI        MunI        NcoI        NdeI        
NheI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SalI        SapI        SbfI        SfcI        SfiI        
SfuI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        Tru9I       
Tth111I     XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

Acc65I        593  GGTACC               G^GTACC
Alw44I         55  GTGCAC               G^TGCAC
AocI         1226  CCTCAGG              CC^T[ACGT]AGG
ApaLI          55  GTGCAC               G^TGCAC
Asp718I       593  GGTACC               G^GTACC
BalI          734  TGGCCA               TGG^CCA
BbsI          976  GAAGAC               GAAGAC(2/6)
BbvCI         196  CCTCAGC              CCTCAGC(-5/-2)
BciVI         604  GGATAC               GTATCC(6/5)
BclI          771  TGATCA               T^GATCA
BlpI          171  GCTGAGC              GC^T[ACGT]AGC
BpmI          329  CTGGAG               CTGGAG(16/14)
Bpu1102I      171  GCTGAGC              GC^T[ACGT]AGC
BpuAI         976  GAAGAC               GAAGAC(2/6)
BsaBI         766  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          631  GGTCTC               GGTCTC(1/5)
BseRI         936  GAGGAG               GAGGAG(10/8)
BsiWI         677  CGTACG               C^GTACG
BsmAI         632  GTCTC                GTCTC(1/5)
Bsp1407I      698  TGTACA               T^GTACA
BsrGI         698  TGTACA               T^GTACA
BstAPI       1015  GCATGTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         134  AGATCC               [GA]^GATC[CT]
Bsu36I       1226  CCTCAGG              CC^T[ACGT]AGG
CelII         171  GCTGAGC              GC^T[ACGT]AGC
DraII         209  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII        775  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          299  CCGCGG               C^C[GA][CT]GG
Eco81I       1226  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      209  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       813  CCAAGG               C^C[AT][AT]GG
FbaI          771  TGATCA               T^GATCA
HgaI          670  GCGTC                GACGC(5/10)
KpnI          593  GGTACC               GGTAC^C
KspI          299  CCGCGG               CCGC^GG
MamI          766  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          134  AGATCC               [GA]^GATC[CT]
MluNI         734  TGGCCA               TGG^CCA
MscI          734  TGGCCA               TGG^CCA
NotI          471  GCGGCCGC             GC^GGCCGC
NspI         1015  GCATGT               [GA]CATG^[CT]
PflMI         885  CCACAGTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         209  GGGACCC              [GA]G^G[AT]CC[CT]
SacII         299  CCGCGG               CCGC^GG
ScaI          527  AGTACT               AGT^ACT
SgrAI         104  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI          213  CCCGGG               CCC^GGG
SspBI         698  TGTACA               T^GTACA
StyI          813  CCAAGG               C^C[AT][AT]GG
TaqI          388  TCGA                 T^CGA
TfiI         1162  GAATC                G^A[AT]TC
Tsp509I      1239  AATT                 ^AATT
TthHB8I       388  TCGA                 T^CGA
Van91I        885  CCACAGTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          394  TCTAGA               T^CTAGA
XcmI          844  CCAAGATATTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         134  AGATCC               [GA]^GATC[CT]
XmaCI         213  CCCGGG               C^CCGGG
XmaI          213  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AspHI          19  GTGCTC               G[AT]GC[AT]^C
               55  GTGCAC              
AvaI           28  CTCGGG               C^[CT]CG[GA]G
              213  CCCGGG              
AvaII          14  GGACC                G^G[AT]CC
              210  GGACC               
BanII         180  GGGCTC               G[GA]GC[CT]^C
              398  GAGCCC              
BfaI           52  CTAG                 C^TAG
              395  CTAG                
BsaAI         675  CACGTA               [CT]AC^GT[GA]
              679  TACGTG              
BsiEI         317  CGGCCG               CG[GA][CT]^CG
              472  CGGCCG              
BsiHKAI        19  GTGCTC               G[AT]GC[AT]^C
               55  GTGCAC              
BsoBI          28  CTCGGG               C^[CT]CG[GA]G
              213  CCCGGG              
BssHII         47  GCGCGC               G^CGCGC
              420  GCGCGC              
BstXI         479  CCAGCCGCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1145  CCAGCTACCTGG        
EagI          317  CGGCCG               C^GGCCG
              472  CGGCCG              
EclXI         317  CGGCCG               C^GGCCG
              472  CGGCCG              
Eco52I        317  CGGCCG               C^GGCCG
              472  CGGCCG              
HphI          335  TCACC                GGTGA(8/7)
             1243  TCACC               
MaeI           52  CTAG                 C^TAG
              395  CTAG                
MaeII         676  ACGT                 A^CGT
              680  ACGT                
MboII         687  GAAGA                GAAGA(8/7)
              976  GAAGA               
MslI          675  CACGTACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1216  CATGCGTATG          
MspA1I        299  CCGCGG               C[AC]G^C[GT]G
              338  CCGCTG              
PleI          332  GAGTC                GAGTC(4/5)
             1041  GAGTC               
SexAI         641  ACCTGGT              A^CC[AT]GGT
             1151  ACCTGGT             
SfaNI         710  GATGC                GCATC(5/9)
              922  GATGC               


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        BanI        BbeI        BbvI        BcnI        
BmyI        BsaHI       BsaJI       BsgI        BsiYI       
BslI        BsmFI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       EaeI        EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       ItaI        
KasI        MaeIII      MboI        MnlI        MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfoI        
TseI        Tsp45I      TspRI       
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