Restriction map for pF1KB6633
 (Linear) (Six-base) MAPSORT of: KIBB6633.seq  Check: 3510  from: 1  to: 1251



KIBB6633.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     147    1251 
   Size:        147    1104
 
Acc65I G'GTAC_C

Cuts at:      0     593    1251 
   Size:        593     658
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     968    1251 
   Size:        968     283
 
AleI CACnn'nnGTG

Cuts at:      0     679    1251 
   Size:        679     572
 
ApaLI G'TGCA_C

Cuts at:      0      55    1251 
   Size:         55    1196
 
AvaI C'yCGr_G

Cuts at:      0      28     213    1251 
   Size:         28     185    1038

  Fragments arranged by size: 

                1038     185      28
 
BanI G'GyrC_C

Cuts at:      0     118     125     217     412     593    1251 
   Size:        118       7      92     195     181     658

  Fragments arranged by size: 

                 658     195     181     118      92       7
 
BanII G_rGCy'C

Cuts at:      0     184     402    1251 
   Size:        184     218     849

  Fragments arranged by size: 

                 849     218     184
 
BbeI G_GCGC'C

Cuts at:      0     122     129     221     416    1251 
   Size:        122       7      92     195     835

  Fragments arranged by size: 

                 835     195     122      92       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     983    1251 
   Size:        983     268
 
BbvCI CC'TCA_GC

Cuts at:      0     197    1251 
   Size:        197    1054
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     598    1251 
   Size:        598     653
 
BclI T'GATC_A

Cuts at:      0     771    1251 
   Size:        771     480
 
BlpI GC'TnA_GC

Cuts at:      0     172    1251 
   Size:        172    1079
 
Bme1580I G_kGCm'C

Cuts at:      0      59    1251 
   Size:         59    1192
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350    1251 
   Size:        350     901
 
Bpu10I CC'TnA_GC

Cuts at:      0     197    1251 
   Size:        197    1054
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     637    1251 
   Size:        637     614
 
BsaAI yAC'GTr

Cuts at:      0     677     681    1251 
   Size:        677       4     570

  Fragments arranged by size: 

                 677     570       4
 
BsaBI GATnn'nnATC

Cuts at:      0     770    1251 
   Size:        770     481
 
BsaHI Gr'CG_yC

Cuts at:      0     119     126     218     413     670    1251 
   Size:        119       7      92     195     257     581

  Fragments arranged by size: 

                 581     257     195     119      92       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     951    1251 
   Size:        951     300
 
BseYI C'CCAG_C

Cuts at:      0     251     478     835    1144    1251 
   Size:        251     227     357     309     107

  Fragments arranged by size: 

                 357     309     251     227     107
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     703    1007    1088    1218    1251 
   Size:        254     449     304      81     130      33

  Fragments arranged by size: 

                 449     304     254     130      81      33
 
BsiEI CG_ry'CG

Cuts at:      0     320     475    1251 
   Size:        320     155     776

  Fragments arranged by size: 

                 776     320     155
 
BsiHKAI G_wGCw'C

Cuts at:      0      23      59    1251 
   Size:         23      36    1192

  Fragments arranged by size: 

                1192      36      23
 
BsiWI C'GTAC_G

Cuts at:      0     677    1251 
   Size:        677     574
 
Bsp1286I G_dGCh'C

Cuts at:      0      23      59     184     402    1251 
   Size:         23      36     125     218     849

  Fragments arranged by size: 

                 849     218     125      36      23
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     147     476     581     923    1251 
   Size:        147     329     105     342     328

  Fragments arranged by size: 

                 342     329     328     147     105
 
BsrFI r'CCGG_y

Cuts at:      0      76     105     319     596    1251 
   Size:         76      29     214     277     655

  Fragments arranged by size: 

                 655     277     214      76      29
 
BsrGI T'GTAC_A

Cuts at:      0     698    1251 
   Size:        698     553
 
BssHII G'CGCG_C

Cuts at:      0      47     420    1251 
   Size:         47     373     831

  Fragments arranged by size: 

                 831     373      47
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1021    1251 
   Size:       1021     230
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     486    1152    1251 
   Size:        486     666      99

  Fragments arranged by size: 

                 666     486      99
 
BstYI r'GATC_y

Cuts at:      0     134    1251 
   Size:        134    1117
 
Bsu36I CC'TnA_GG

Cuts at:      0    1227    1251 
   Size:       1227      24
 
BtgI C'CryG_G

Cuts at:      0     299    1251 
   Size:        299     952
 
BtsI GCAGTG_nn'

Cuts at:      0      57    1251 
   Size:         57    1194
 
DraIII CAC_nnn'GTG

Cuts at:      0     780    1251 
   Size:        780     471
 
EaeI y'GGCC_r

Cuts at:      0      78     317     342     472     647     734    1251 
   Size:         78     239      25     130     175      87     517

  Fragments arranged by size: 

                 517     239     175     130      87      78      25
 
EagI C'GGCC_G

Cuts at:      0     317     472    1251 
   Size:        317     155     779

  Fragments arranged by size: 

                 779     317     155
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     237     733    1251 
   Size:        237     496     518

  Fragments arranged by size: 

                 518     496     237
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     968    1251 
   Size:        350     618     283

  Fragments arranged by size: 

                 618     350     283
 
EcoO109I rG'GnC_Cy

Cuts at:      0     210    1251 
   Size:        210    1041
 
HaeII r_GCGC'y

Cuts at:      0     122     129     204     221     416     442    1251 
   Size:        122       7      75      17     195      26     809

  Fragments arranged by size: 

                 809     195     122      75      26      17       7
 
KasI G'GCGC_C

Cuts at:      0     118     125     217     412    1251 
   Size:        118       7      92     195     839

  Fragments arranged by size: 

                 839     195     118      92       7
 
KpnI G_GTAC'C

Cuts at:      0     597    1251 
   Size:        597     654
 
MscI TGG'CCA

Cuts at:      0     736    1251 
   Size:        736     515
 
MslI CAynn'nnrTG

Cuts at:      0     679    1220    1251 
   Size:        679     541      31

  Fragments arranged by size: 

                 679     541      31
 
MspA1I CmG'CkG

Cuts at:      0     301     340    1251 
   Size:        301      39     911

  Fragments arranged by size: 

                 911     301      39
 
NaeI GCC'GGC

Cuts at:      0      78     107     321    1251 
   Size:         78      29     214     930

  Fragments arranged by size: 

                 930     214      78      29
 
NarI GG'CG_CC

Cuts at:      0     119     126     218     413    1251 
   Size:        119       7      92     195     838

  Fragments arranged by size: 

                 838     195     119      92       7
 
NgoMIV G'CCGG_C

Cuts at:      0      76     105     319    1251 
   Size:         76      29     214     932

  Fragments arranged by size: 

                 932     214      76      29
 
NotI GC'GGCC_GC

Cuts at:      0     472    1251 
   Size:        472     779
 
NspI r_CATG'y

Cuts at:      0    1019    1251 
   Size:       1019     232
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     891    1251 
   Size:        891     360
 
PpuMI rG'GwC_Cy

Cuts at:      0     210    1251 
   Size:        210    1041
 
SacII CC_GC'GG

Cuts at:      0     302    1251 
   Size:        302     949
 
SanDI GG'GwC_CC

Cuts at:      0     210    1251 
   Size:        210    1041
 
ScaI AGT'ACT

Cuts at:      0     529    1251 
   Size:        529     722
 
SexAI A'CCwGG_T

Cuts at:      0     641    1151    1251 
   Size:        641     510     100

  Fragments arranged by size: 

                 641     510     100
 
SfoI GGC'GCC

Cuts at:      0     120     127     219     414    1251 
   Size:        120       7      92     195     837

  Fragments arranged by size: 

                 837     195     120      92       7
 
SgrAI Cr'CCGG_yG

Cuts at:      0     105    1251 
   Size:        105    1146
 
SmaI CCC'GGG

Cuts at:      0     215    1251 
   Size:        215    1036
 
StyI C'CwwG_G

Cuts at:      0     813    1251 
   Size:        813     438
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     946    1251 
   Size:        946     305
 
TatI w'GTAC_w

Cuts at:      0     527     698     739    1018    1251 
   Size:        527     171      41     279     233

  Fragments arranged by size: 

                 527     279     233     171      41
 
XbaI T'CTAG_A

Cuts at:      0     394    1251 
   Size:        394     857
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     851    1251 
   Size:        851     400
 
XmaI C'CCGG_G

Cuts at:      0     213    1251 
   Size:        213    1038
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AleI    ApaLI     AvaI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI     BlpI Bme1580I     BpmI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspMI    BsrFI    BsrGI   BssHII
   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI
     EagI     EciI  Eco57MI EcoO109I    HaeII     KasI     KpnI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NotI     NspI    PflMI
    PpuMI    SacII    SanDI     ScaI    SexAI     SfoI    SgrAI     SmaI
     StyI    TaqII     TatI     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BcgI    BfrBI     BglI    BglII    BmgBI
     BmrI     BmtI     BplI    BpuEI    BsaWI    BsaXI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BssSI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI     DraI     DrdI     EarI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     MfeI     MluI     MmeI     NcoI     NdeI     NheI
     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     SfcI     SfiI
     SgfI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XhoI     XmnI     ZraI
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