Restriction map for pF1KB0951
##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbvCI       BclI        BfrI        
BglI        BlnI        BlpI        BmyI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DdeI        DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        MvnI        
MwoI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tru9I       Tth111I     Van91I      XbaI        XcmI        
XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcsI          513  GAATTC               [GA]^AATT[CT]
AlwI          106  GATCC                GGATC(4/5)
ApoI          513  GAATTC               [GA]^AATT[CT]
AvaII         231  GGTCC                G^G[AT]CC
BanI          333  GGTGCC               G^G[CT][GA]CC
BbsI           54  GAAGAC               GAAGAC(2/6)
BbvI          536  GCTGC                GCAGC(8/12)
BcgI          525  GCAAGAGCGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI          37  GGATAC               GTATCC(6/5)
BglII         363  AGATCT               A^GATCT
BpmI          371  CTCCAG               CTGGAG(16/14)
BpuAI          54  GAAGAC               GAAGAC(2/6)
BsiYI         165  CCTACATCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          165  CCTACATCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          203  GCATTC               GAATGC(1/-1)
BsrI          337  CCAGT                ACTGG(1/-1)
BstYI         363  AGATCT               [GA]^GATC[CT]
Cac8I         408  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr13I        231  GGTCC                G^G[ACGT]CC
EcoRI         513  GAATTC               G^AATTC
HaeIII        316  GGCC                 GG^CC
HapII         330  CCGG                 C^CGG
HgaI          531  GCGTC                GACGC(5/10)
HpaII         330  CCGG                 C^CGG
MflI          363  AGATCT               [GA]^GATC[CT]
MslI          235  CATGGTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          330  CCGG                 C^CGG
PaeR7I        449  CTCGAG               C^TCGAG
PvuII         460  CAGCTG               CAG^CTG
Sau96I        231  GGTCC                G^G[ACGT]CC
SfaNI         473  GCATC                GCATC(5/9)
SmaI          329  CCCGGG               CCC^GGG
TaqI          450  TCGA                 T^CGA
TfiI          456  GAATC                G^A[AT]TC
TseI          536  GCTGC                G^C[AT]GC
TthHB8I       450  TCGA                 T^CGA
XhoI          449  CTCGAG               C^TCGAG
XhoII         363  AGATCT               [GA]^GATC[CT]
XmaCI         329  CCCGGG               C^CCGGG
XmaI          329  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AvaI          329  CCCGGG               C^[CT]CG[GA]G
              449  CTCGAG              
BcnI          329  CCCGG                CC^[GC]GG
              330  CCGGG               
BfaI            7  CTAG                 C^TAG
              346  CTAG                
BsoBI         329  CCCGGG               C^[CT]CG[GA]G
              449  CTCGAG              
BstNI         419  CCTGG                CC^[AT]GG
              492  CCAGG               
DpnII         106  GATC                 ^GATC
              364  GATC                
DsaI          234  CCATGG               C^C[GA][CT]GG
              318  CCATGG              
EcoRII        419  CCTGG                ^CC[AT]GG
              492  CCAGG               
EcoT14I       234  CCATGG               C^C[AT][AT]GG
              318  CCATGG              
Fnu4HI          3  GCCGC                GC^[ACGT]GC
              536  GCTGC               
ItaI            3  GCCGC                GC^[ACGT]GC
              536  GCTGC               
MaeI            7  CTAG                 C^TAG
              346  CTAG                
MaeIII        139  GTGAC                ^GT[ACGT]AC
              259  GTCAC               
MboI          106  GATC                 ^GATC
              364  GATC                
MboII          54  GAAGA                GAAGA(8/7)
              476  TCTTC               
MspA1I         42  CCGCTG               C[AC]G^C[GT]G
              460  CAGCTG              
MvaI          419  CCTGG                CC^[AT]GG
              492  CCAGG               
NciI          329  CCCGG                CC^[GC]GG
              330  CCGGG               
NcoI          234  CCATGG               C^CATGG
              318  CCATGG              
NdeII         106  GATC                 ^GATC
              364  GATC                
NlaIV         230  GGGTCC               GG[ACGT]^[ACGT]CC
              333  GGTGCC              
Sau3AI        106  GATC                 ^GATC
              364  GATC                
SfcI           15  CTACAG               C^T[GA][CT]AG
              110  CTACAG              
SmlI          449  CTCGAG               C^T[CT][GA]AG
              467  CTCAAG              
StyI          234  CCATGG               C^C[AT][AT]GG
              318  CCATGG              
Tsp45I        139  GTGAC                ^GT[GC]AC
              259  GTCAC               
Tsp509I        74  AATT                 ^AATT
              514  AATT                
TspRI         113  CAGTG                CA[GC]TG[ACGT][ACGT]^
              338  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AciI        AfaI        AluI        BsaJI       BssKI       
BstF5I      FokI        HinfI       HphI        MnlI        
NlaIII      PleI        RsaI        ScrFI       
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