Restriction map for pF1KB0943
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        AluI        AocI        
Aor51HI     ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        HphI        KasI        KpnI        KspI        
MaeII       MaeIII      MamI        MfeI        MluI        
MroI        MspA1I      MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tsp45I      Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Alw44I        133  GTGCAC               G^TGCAC
AlwI          495  GGATC                GGATC(4/5)
ApaI          415  GGGCCC               GGGCC^C
ApaLI         133  GTGCAC               G^TGCAC
AvrII          51  CCTAGG               C^CTAGG
BbsI          184  GAAGAC               GAAGAC(2/6)
BcnI          241  CCCGG                CC^[GC]GG
BlnI           51  CCTAGG               C^CTAGG
BpmI          328  CTGGAG               CTGGAG(16/14)
BpuAI         184  GAAGAC               GAAGAC(2/6)
BsgI           43  CTGCAC               GTGCAG(16/14)
BsmAI          10  GTCTC                GTCTC(1/5)
BsrI          283  ACTGG                ACTGG(1/-1)
BssSI         299  CACGAG               CACGAG(-5/-1)
BstBI         103  TTCGAA               TT^CGAA
BstYI         495  GGATCT               [GA]^GATC[CT]
CfoI          424  GCGC                 GCG^C
DpnII         496  GATC                 ^GATC
DsaI           92  CCATGG               C^C[GA][CT]GG
EarI           79  CTCTTC               CTCTTC(1/4)
EcoNI         383  CCTTCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII         242  CCGG                 C^CGG
HhaI          424  GCGC                 GCG^C
HinP1I        424  GCGC                 G^CGC
HinfI          59  GATTC                G^A[ACGT]TC
HpaII         242  CCGG                 C^CGG
Ksp632I        79  CTCTTC               CTCTTC(1/4)
MboI          496  GATC                 ^GATC
MflI          495  GGATCT               [GA]^GATC[CT]
MseI          341  TTAA                 T^TAA
MslI          124  CATGGCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          242  CCGG                 C^CGG
NciI          241  CCCGG                CC^[GC]GG
NcoI           92  CCATGG               C^CATGG
NdeII         496  GATC                 ^GATC
NspV          103  TTCGAA               TT^CGAA
PpuMI         163  AGGACCT              [GA]G^G[AT]CC[CT]
Sau3AI        496  GATC                 ^GATC
SexAI         204  ACCTGGT              A^CC[AT]GGT
SfcI           14  CTGTAG               C^T[GA][CT]AG
SfuI          103  TTCGAA               TT^CGAA
TfiI           59  GATTC                G^A[AT]TC
Tru9I         341  TTAA                 T^TAA
XhoII         495  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwNI           8  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              200  CAGAACCTG           
AvaII         164  GGACC                G^G[AT]CC
              472  GGACC               
BalI          217  TGGCCA               TGG^CCA
              255  TGGCCA              
BanII         264  GGGCTC               G[GA]GC[CT]^C
              415  GGGCCC              
BfaI           52  CTAG                 C^TAG
              116  CTAG                
BglI          248  GCCATCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              257  GCCAAGTGGGC         
BspHI          20  TCATGA               T^CATGA
              150  TCATGA              
BstF5I         32  CATCC                GGATG(2/0)
               48  CATCC               
Cac8I         393  GCCCGC               GC[ACGT]^[ACGT]GC
              397  GCCCGC              
DraII         163  AGGACCT              [GA]G^G[ACGT]CC[CT]
              415  GGGCCCC             
EcoO109I      163  AGGACCT              [GA]G^G[ACGT]CC[CT]
              415  GGGCCCC             
FokI           32  CATCC                GGATG(9/13)
               48  CATCC               
MaeI           52  CTAG                 C^TAG
              116  CTAG                
MluNI         217  TGGCCA               TGG^CCA
              255  TGGCCA              
MnlI          358  CCTC                 CCTC(7/6)
              302  GAGG                
MscI          217  TGGCCA               TGG^CCA
              255  TGGCCA              
PflMI         226  CCATCTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              475  CCACCTTATGG         
RcaI           20  TCATGA               T^CATGA
              150  TCATGA              
TaqI          104  TCGA                 T^CGA
              291  TCGA                
TspRI         214  CAGTG                CA[GC]TG[ACGT][ACGT]^
              501  CACTG               
TthHB8I       104  TCGA                 T^CGA
              291  TCGA                
Van91I        226  CCATCTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              475  CCACCTTATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AciI        AspHI       BbvI        BmyI        BsaJI       
BsiHKAI     BsiYI       BslI        Bsp1286I    BssKI       
BstNI       Cfr13I      DdeI        EaeI        EcoRII      
EcoT14I     FauI        Fnu4HI      HaeIII      ItaI        
MboII       MvaI        MwoI        NlaIII      NlaIV       
Sau96I      ScrFI       SfaNI       StyI        TseI        
Tsp509I     
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