Restriction map for pF1KB0943
 (Linear) (Six-base) MAPSORT of: KIBB0943.seq  Check: 3606  from: 1  to: 510



KIBB0943.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     510 
   Size:        203     307
 
AlwNI CAG_nnn'CTG

Cuts at:      0      13     205     510 
   Size:         13     192     305

  Fragments arranged by size: 

                 305     192      13
 
ApaI G_GGCC'C

Cuts at:      0     419     510 
   Size:        419      91
 
ApaLI G'TGCA_C

Cuts at:      0     133     510 
   Size:        133     377
 
AvrII C'CTAG_G

Cuts at:      0      51     510 
   Size:         51     459
 
BanII G_rGCy'C

Cuts at:      0     268     419     510 
   Size:        268     151      91

  Fragments arranged by size: 

                 268     151      91
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     191     510 
   Size:        191     319
 
BglI GCCn_nnn'nGGC

Cuts at:      0     254     263     510 
   Size:        254       9     247

  Fragments arranged by size: 

                 254     247       9
 
Bme1580I G_kGCm'C

Cuts at:      0     137     419     510 
   Size:        137     282      91

  Fragments arranged by size: 

                 282     137      91
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     510 
   Size:        349     161
 
BseYI C'CCAG_C

Cuts at:      0     271     420     510 
   Size:        271     149      90

  Fragments arranged by size: 

                 271     149      90
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     510 
   Size:         28     482
 
BsiHKAI G_wGCw'C

Cuts at:      0     137     213     297     377     510 
   Size:        137      76      84      80     133

  Fragments arranged by size: 

                 137     133      84      80      76
 
Bsp1286I G_dGCh'C

Cuts at:      0     137     213     268     297     377     419     510 
   Size:        137      76      55      29      80      42      91

  Fragments arranged by size: 

                 137      91      80      76      55      42      29
 
BspHI T'CATG_A

Cuts at:      0      20     150     510 
   Size:         20     130     360

  Fragments arranged by size: 

                 360     130      20
 
BssSI C'ACGA_G

Cuts at:      0     299     510 
   Size:        299     211
 
BstBI TT'CG_AA

Cuts at:      0     104     510 
   Size:        104     406
 
BstYI r'GATC_y

Cuts at:      0     495     510 
   Size:        495      15
 
BtgI C'CryG_G

Cuts at:      0      92     510 
   Size:         92     418
 
BtsI GCAGTG_nn'

Cuts at:      0     500     510 
   Size:        500      10
 
EaeI y'GGCC_r

Cuts at:      0     126     217     255     510 
   Size:        126      91      38     255

  Fragments arranged by size: 

                 255     126      91      38
 
EarI CTCTTCn'nnn_

Cuts at:      0      85     510 
   Size:         85     425
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     349     510 
   Size:        203     146     161

  Fragments arranged by size: 

                 203     161     146
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     387     510 
   Size:        387     123
 
EcoO109I rG'GnC_Cy

Cuts at:      0     164     416     510 
   Size:        164     252      94

  Fragments arranged by size: 

                 252     164      94
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     245     510 
   Size:        245     265
 
MscI TGG'CCA

Cuts at:      0     219     257     510 
   Size:        219      38     253

  Fragments arranged by size: 

                 253     219      38
 
MslI CAynn'nnrTG

Cuts at:      0     128     510 
   Size:        128     382
 
NcoI C'CATG_G

Cuts at:      0      92     510 
   Size:         92     418
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     232     481     510 
   Size:        232     249      29

  Fragments arranged by size: 

                 249     232      29
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      27     510 
   Size:         27     483
 
PpuMI rG'GwC_Cy

Cuts at:      0     164     510 
   Size:        164     346
 
PspOMI G'GGCC_C

Cuts at:      0     415     510 
   Size:        415      95
 
SexAI A'CCwGG_T

Cuts at:      0     204     510 
   Size:        204     306
 
SfcI C'TryA_G

Cuts at:      0      14     510 
   Size:         14     496
 
StyI C'CwwG_G

Cuts at:      0      51      92     220     388     510 
   Size:         51      41     128     168     122

  Fragments arranged by size: 

                 168     128     122      51      41
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     174     510 
   Size:        174     336
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI    ApaLI    AvrII    BanII     BbsI     BglI
 Bme1580I     BpmI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BssSI
    BstBI    BstYI     BtgI     BtsI     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I     FalI     MscI     MslI     NcoI    PflMI     PpiI    PpuMI
   PspOMI    SexAI     SfcI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApoI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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