Restriction map for pF1KB0933
##################################
# NO CUT ENZYMES (  189 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbvCI       BbvI        BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
CelII       CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       HphI        ItaI        KasI        
KpnI        KspI        MaeII       MaeIII      MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        TseI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AfaI           89  GTAC                 GT^AC
AlwNI          52  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          409  CCTCAGG              CC^T[ACGT]AGG
ApaI          415  GGGCCC               GGGCC^C
AvaI          260  CTCGAG               C^[CT]CG[GA]G
BfaI          244  CTAG                 C^TAG
BpmI          325  CTGGAG               CTGGAG(16/14)
BsgI          233  GTGCAG               GTGCAG(16/14)
BsmFI         344  GTCCC                GGGAC(10/14)
BsoBI         260  CTCGAG               C^[CT]CG[GA]G
BssKI         424  CCTGG                ^CC[ACGT]GG
BstNI         424  CCTGG                CC^[AT]GG
Bsu36I        409  CCTCAGG              CC^T[ACGT]AGG
DsaI          399  CCGTGG               C^C[GA][CT]GG
EarI          121  CTCTTC               CTCTTC(1/4)
Eco81I        409  CCTCAGG              CC^T[ACGT]AGG
EcoRII        424  CCTGG                ^CC[AT]GG
Ksp632I       121  CTCTTC               CTCTTC(1/4)
MaeI          244  CTAG                 C^TAG
MspA1I        370  CAGCTG               C[AC]G^C[GT]G
MvaI          424  CCTGG                CC^[AT]GG
MwoI          384  GCACAGTAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI          356  ACATGC               [GA]CATG^[CT]
PaeR7I        260  CTCGAG               C^TCGAG
PvuII         370  CAGCTG               CAG^CTG
RsaI           89  GTAC                 GT^AC
ScrFI         424  CCTGG                CC^[ACGT]GG
SfaNI          33  GATGC                GCATC(5/9)
SfcI          367  CTACAG               C^T[GA][CT]AG
SmlI          260  CTCGAG               C^T[CT][GA]AG
SspI           40  AATATT               AAT^ATT
TaqI          261  TCGA                 T^CGA
TspRI         236  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       261  TCGA                 T^CGA
XhoI          260  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcsI          166  GAATTT               [GA]^AATT[CT]
              216  GAATTT              
ApoI          166  GAATTT               [GA]^AATT[CT]
              216  GAATTT              
AspHI           3  GAGCTC               G[AT]GC[AT]^C
              226  GAGCTC              
AvaII         396  GGACC                G^G[AT]CC
              403  GGACC               
BbsI          247  GAAGAC               GAAGAC(2/6)
              103  GTCTTC              
BpuAI         247  GAAGAC               GAAGAC(2/6)
              103  GTCTTC              
BsaJI         399  CCGTGG               C^C[ACGT][ACGT]GG
              424  CCTGGG              
BsiHKAI         3  GAGCTC               G[AT]GC[AT]^C
              226  GAGCTC              
BsrI          361  CCAGT                ACTGG(1/-1)
              378  CCAGT               
BstF5I        108  CATCC                GGATG(2/0)
               32  GGATG               
Cac8I         291  GCACGC               GC[ACGT]^[ACGT]GC
              372  GCTGGC              
DraII         414  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              415  GGGCCCC             
EcoO109I      414  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              415  GGGCCCC             
FokI          108  CATCC                GGATG(9/13)
               32  GGATG               
HaeIII        375  GGCC                 GG^CC
              416  GGCC                
HinfI          47  GATTC                G^A[ACGT]TC
               54  GAATC               
MseI          193  TTAA                 T^TAA
              317  TTAA                
SacI            3  GAGCTC               GAGCT^C
              226  GAGCTC              
TfiI           47  GATTC                G^A[AT]TC
               54  GAATC               
Tru9I         193  TTAA                 T^TAA
              317  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        BanII       BmyI        BsiYI       BslI        
Bsp1286I    Cfr13I      DdeI        MboII       MnlI        
NlaIII      NlaIV       Sau96I      Tsp509I     
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