Restriction map for pF1KB0927
 (Linear) (Six-base) MAPSORT of: KIBB0927.seq  Check: 6727  from: 1  to: 399



KIBB0927.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AflIII A'CryG_T

Cuts at:      0     339     399 
   Size:        339      60
 
BanI G'GyrC_C

Cuts at:      0     103     399 
   Size:        103     296
 
BanII G_rGCy'C

Cuts at:      0     233     366     399 
   Size:        233     133      33

  Fragments arranged by size: 

                 233     133      33
 
BbeI G_GCGC'C

Cuts at:      0     107     399 
   Size:        107     292
 
BglII A'GATC_T

Cuts at:      0      29     399 
   Size:         29     370
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     322     399 
   Size:        322      77
 
BmtI G_CTAG'C

Cuts at:      0     383     399 
   Size:        383      16
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     311     399 
   Size:         97     214      88

  Fragments arranged by size: 

                 214      97      88
 
Bpu10I CC'TnA_GC

Cuts at:      0     186     399 
   Size:        186     213
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     212     266     399 
   Size:        212      54     133

  Fragments arranged by size: 

                 212     133      54
 
BsaHI Gr'CG_yC

Cuts at:      0     104     199     399 
   Size:        104      95     200

  Fragments arranged by size: 

                 200     104      95
 
BsaWI w'CCGG_w

Cuts at:      0     239     399 
   Size:        239     160
 
BseYI C'CCAG_C

Cuts at:      0     211     333     399 
   Size:        211     122      66

  Fragments arranged by size: 

                 211     122      66
 
BsiHKAI G_wGCw'C

Cuts at:      0     233     399 
   Size:        233     166
 
Bsp1286I G_dGCh'C

Cuts at:      0     233     366     399 
   Size:        233     133      33

  Fragments arranged by size: 

                 233     133      33
 
BspEI T'CCGG_A

Cuts at:      0     239     399 
   Size:        239     160
 
BsrFI r'CCGG_y

Cuts at:      0     100     399 
   Size:        100     299
 
BstYI r'GATC_y

Cuts at:      0      29     399 
   Size:         29     370
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      71     399 
   Size:         71     328
 
EaeI y'GGCC_r

Cuts at:      0      98     176     307     369     399 
   Size:         98      78     131      62      30

  Fragments arranged by size: 

                 131      98      78      62      30
 
EarI CTCTTCn'nnn_

Cuts at:      0     290     399 
   Size:        290     109
 
EcoICRI GAG'CTC

Cuts at:      0     231     399 
   Size:        231     168
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     311     399 
   Size:         97     214      88

  Fragments arranged by size: 

                 214      97      88
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     115     399 
   Size:        115     284
 
HaeII r_GCGC'y

Cuts at:      0     107     399 
   Size:        107     292
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     146     178     234     266     399 
   Size:        146      32      56      32     133

  Fragments arranged by size: 

                 146     133      56      32      32
 
HindIII A'AGCT_T

Cuts at:      0     183     399 
   Size:        183     216
 
KasI G'GCGC_C

Cuts at:      0     103     399 
   Size:        103     296
 
MspA1I CmG'CkG

Cuts at:      0     215     399 
   Size:        215     184
 
NaeI GCC'GGC

Cuts at:      0     102     399 
   Size:        102     297
 
NarI GG'CG_CC

Cuts at:      0     104     399 
   Size:        104     295
 
NgoMIV G'CCGG_C

Cuts at:      0     100     399 
   Size:        100     299
 
NheI G'CTAG_C

Cuts at:      0     379     399 
   Size:        379      20
 
NspI r_CATG'y

Cuts at:      0     343     399 
   Size:        343      56
 
PasI CC'CwG_GG

Cuts at:      0     193     399 
   Size:        193     206
 
PciI A'CATG_T

Cuts at:      0     339     399 
   Size:        339      60
 
PfoI T'CCnGG_A

Cuts at:      0     113     399 
   Size:        113     286
 
SacI G_AGCT'C

Cuts at:      0     233     399 
   Size:        233     166
 
SapI GCTCTTCn'nnn_

Cuts at:      0     290     399 
   Size:        290     109
 
SfoI GGC'GCC

Cuts at:      0     105     399 
   Size:        105     294
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     255     355     399 
   Size:        255     100      44

  Fragments arranged by size: 

                 255     100      44
 

 Enzymes that do cut:

   AflIII     BanI    BanII     BbeI    BglII     BmrI     BmtI     BpmI
   Bpu10I     BsaI    BsaHI    BsaWI    BseYI  BsiHKAI Bsp1286I    BspEI
    BsrFI    BstYI    BtgZI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
    HaeII    Hin4I  HindIII     KasI   MspA1I     NaeI     NarI   NgoMIV
     NheI     NspI     PasI     PciI     PfoI     SacI     SapI     SfoI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BplI    BpuEI    BsaAI    BsaBI    BsaXI    BseRI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NcoI     NdeI     NotI     NruI     NsiI     PacI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
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