Restriction map for pF1KB0919
##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BanI        BbeI        BbrPI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeIII      MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MunI        NarI        NcoI        NdeI        NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccII          22  CGCG                 CG^CG
AocI          347  CCTCAGG              CC^T[ACGT]AGG
AspHI         262  GAGCTC               G[AT]GC[AT]^C
AvaI          171  CCCGGG               C^[CT]CG[GA]G
AvaII         285  GGACC                G^G[AT]CC
BamHI          99  GGATCC               G^GATCC
BbsI          127  GAAGAC               GAAGAC(2/6)
BbvCI         246  GCTGAGG              CCTCAGC(-5/-2)
BglI          202  GCCTACGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         127  GAAGAC               GAAGAC(2/6)
BsaAI         205  TACGTG               [CT]AC^GT[GA]
BseRI         345  CTCCTC               GAGGAG(10/8)
BsiHKAI       262  GAGCTC               G[AT]GC[AT]^C
BsoBI         171  CCCGGG               C^[CT]CG[GA]G
BsrBI         292  GAGCGG               CCGCTC(-3/-3)
BstUI          22  CGCG                 CG^CG
Bsu36I        347  CCTCAGG              CC^T[ACGT]AGG
DsaI           53  CCACGG               C^C[GA][CT]GG
Eco81I        347  CCTCAGG              CC^T[ACGT]AGG
EcoT14I         8  CCAAGG               C^C[AT][AT]GG
HaeII          45  AGCGCC               [GA]GCGC^[CT]
HphI          110  TCACC                GGTGA(8/7)
MboII         127  GAAGA                GAAGA(8/7)
MspA1I        220  CAGCGG               C[AC]G^C[GT]G
MvnI           22  CGCG                 CG^CG
PpuMI         284  AGGACCT              [GA]G^G[AT]CC[CT]
SacI          262  GAGCTC               GAGCT^C
SfaNI         160  GATGC                GCATC(5/9)
SmaI          171  CCCGGG               CCC^GGG
StyI            8  CCAAGG               C^C[AT][AT]GG
TspRI         104  CAGTG                CA[GC]TG[ACGT][ACGT]^
XmaCI         171  CCCGGG               C^CCGGG
XmaI          171  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AluI          182  AGCT                 AG^CT
              263  AGCT                
AlwNI          64  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              220  CAGCGGCTG           
BanII           3  GGGCTC               G[GA]GC[CT]^C
              262  GAGCTC              
BmyI            3  GGGCTC               G[AGT]GC[ACT]^C
              262  GAGCTC              
BpmI           70  CTGGAG               CTGGAG(16/14)
              289  CTGGAG              
Bsp1286I        3  GGGCTC               G[AGT]GC[ACT]^C
              262  GAGCTC              
BsrI          103  CCAGT                ACTGG(1/-1)
              136  CCAGT               
BstYI          65  AGATCC               [GA]^GATC[CT]
               99  GGATCC              
DdeI          247  CTGAG                C^T[ACGT]AG
              348  CTCAG               
DpnII          66  GATC                 ^GATC
              100  GATC                
DraII         167  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              284  AGGACCT             
EcoO109I      167  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              284  AGGACCT             
MaeII         206  ACGT                 A^CGT
              242  ACGT                
MboI           66  GATC                 ^GATC
              100  GATC                
MflI           65  AGATCC               [GA]^GATC[CT]
               99  GGATCC              
NaeI           90  GCCGGC               GCC^GGC
              277  GCCGGC              
NdeII          66  GATC                 ^GATC
              100  GATC                
NgoMIV         90  GCCGGC               G^CCGGC
              277  GCCGGC              
PstI          226  CTGCAG               CTGCA^G
              268  CTGCAG              
Sau3AI         66  GATC                 ^GATC
              100  GATC                
SfcI          226  CTGCAG               C^T[GA][CT]AG
              268  CTGCAG              
XhoII          65  AGATCC               [GA]^GATC[CT]
               99  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AlwI        BbvI        BcnI        BsaJI       
BsiYI       BslI        BsrFI       BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr10I      Cfr13I      
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MnlI        MspI        MvaI        MwoI        NciI        
NlaIV       Sau96I      ScrFI       TseI        
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