Restriction map for pF1KB0919
 (Linear) (Six-base) MAPSORT of: KIBB0919.seq  Check: 1088  from: 1  to: 354



KIBB0919.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     129     354 
   Size:        129     225
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      91     123     354 
   Size:         91      32     231

  Fragments arranged by size: 

                 231      91      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      69     225     354 
   Size:         69     156     129

  Fragments arranged by size: 

                 156     129      69
 
AvaI C'yCGr_G

Cuts at:      0     171     354 
   Size:        171     183
 
BamHI G'GATC_C

Cuts at:      0      99     354 
   Size:         99     255
 
BanII G_rGCy'C

Cuts at:      0       7     266     354 
   Size:          7     259      88

  Fragments arranged by size: 

                 259      88       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     134     354 
   Size:        134     220
 
BbvCI CC'TCA_GC

Cuts at:      0     247     354 
   Size:        247     107
 
BglI GCCn_nnn'nGGC

Cuts at:      0     208     354 
   Size:        208     146
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     130     354 
   Size:        130     224
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     199     354 
   Size:        199     155
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     310     354 
   Size:         91     219      44

  Fragments arranged by size: 

                 219      91      44
 
Bpu10I CC'TnA_GC

Cuts at:      0     247     354 
   Size:        247     107
 
BsaAI yAC'GTr

Cuts at:      0     207     354 
   Size:        207     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     336     354 
   Size:        336      18
 
BseYI C'CCAG_C

Cuts at:      0      42     354 
   Size:         42     312
 
BsiHKAI G_wGCw'C

Cuts at:      0     266     354 
   Size:        266      88
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     266     354 
   Size:          7     259      88

  Fragments arranged by size: 

                 259      88       7
 
BsrBI CCG'CTC

Cuts at:      0     294     354 
   Size:        294      60
 
BsrFI r'CCGG_y

Cuts at:      0      90     277     327     354 
   Size:         90     187      50      27

  Fragments arranged by size: 

                 187      90      50      27
 
BstYI r'GATC_y

Cuts at:      0      65      99     354 
   Size:         65      34     255

  Fragments arranged by size: 

                 255      65      34
 
Bsu36I CC'TnA_GG

Cuts at:      0     348     354 
   Size:        348       6
 
BtgI C'CryG_G

Cuts at:      0      53     354 
   Size:         53     301
 
EcoICRI GAG'CTC

Cuts at:      0     264     354 
   Size:        264      90
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     129     310     354 
   Size:         91      38     181      44

  Fragments arranged by size: 

                 181      91      44      38
 
EcoO109I rG'GnC_Cy

Cuts at:      0     168     285     354 
   Size:        168     117      69

  Fragments arranged by size: 

                 168     117      69
 
HaeII r_GCGC'y

Cuts at:      0      49     354 
   Size:         49     305
 
MspA1I CmG'CkG

Cuts at:      0     222     354 
   Size:        222     132
 
NaeI GCC'GGC

Cuts at:      0      92     279     354 
   Size:         92     187      75

  Fragments arranged by size: 

                 187      92      75
 
NgoMIV G'CCGG_C

Cuts at:      0      90     277     354 
   Size:         90     187      77

  Fragments arranged by size: 

                 187      90      77
 
PfoI T'CCnGG_A

Cuts at:      0      68     354 
   Size:         68     286
 
PpuMI rG'GwC_Cy

Cuts at:      0     285     354 
   Size:        285      69
 
PstI C_TGCA'G

Cuts at:      0     230     272     354 
   Size:        230      42      82

  Fragments arranged by size: 

                 230      82      42
 
SacI G_AGCT'C

Cuts at:      0     266     354 
   Size:        266      88
 
SfcI C'TryA_G

Cuts at:      0     226     268     354 
   Size:        226      42      86

  Fragments arranged by size: 

                 226      86      42
 
SmaI CCC'GGG

Cuts at:      0     173     354 
   Size:        173     181
 
StyI C'CwwG_G

Cuts at:      0       8     354 
   Size:          8     346
 
XmaI C'CCGG_G

Cuts at:      0     171     354 
   Size:        171     183
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     AvaI    BamHI    BanII     BbsI    BbvCI
     BglI     BmrI     BplI     BpmI   Bpu10I    BsaAI    BseRI    BseYI
  BsiHKAI Bsp1286I    BsrBI    BsrFI    BstYI   Bsu36I     BtgI  EcoICRI
  Eco57MI EcoO109I    HaeII   MspA1I     NaeI   NgoMIV     PfoI    PpuMI
     PstI     SacI     SfcI     SmaI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BanI     BbeI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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