Restriction map for pF1KB6624
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       BalI        BanI        BbeI        
BbrPI       BcgI        BciVI       BclI        BfrI        
BglI        BsaAI       BsaBI       BsaHI       BsaI        
BseRI       BsiWI       BsoBI       Bsp1407I    BspDI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       BstBI       BstEII      BstPI       BstXI       
Bsu36I      CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        KasI        
KpnI        Ksp632I     MaeII       MamI        MfeI        
MluI        MluNI       MscI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RsrII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII          69  CGCG                 CG^CG
AccIII       1150  TCCGGA               T^CCGGA
AcsI          178  AAATTC               [GA]^AATT[CT]
ApoI          178  AAATTC               [GA]^AATT[CT]
Asp700I       174  GAACAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII         990  CCTAGG               C^CTAGG
BbvCI         690  GCTGAGG              CCTCAGC(-5/-2)
BcnI           86  CCGGG                CC^[GC]GG
BlnI          990  CCTAGG               C^CTAGG
BlpI          951  GCTCAGC              GC^T[ACGT]AGC
BpmI          601  CTGGAG               CTGGAG(16/14)
Bpu1102I      951  GCTCAGC              GC^T[ACGT]AGC
BseAI        1150  TCCGGA               T^CCGGA
BsiEI          94  CGGTCG               CG[GA][CT]^CG
BsmBI         185  GAGACG               CGTCTC(1/5)
BsmI          850  GCATTC               GAATGC(1/-1)
BspEI        1150  TCCGGA               T^CCGGA
Bst1107I     1291  GTATAC               GTA^TAC
BstUI          69  CGCG                 CG^CG
BstZ17I      1291  GTATAC               GTA^TAC
CelII         951  GCTCAGC              GC^T[ACGT]AGC
DrdI          154  GACAAGGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           68  CCGCGG               C^C[GA][CT]GG
EcoT22I       291  ATGCAT               ATGCA^T
FauI          630  GCGGG                CCCGC(4/6)
HincII       1075  GTTGAC               GT[CT]^[GA]AC
HindII       1075  GTTGAC               GT[CT]^[GA]AC
KspI           68  CCGCGG               CCGC^GG
MroI         1150  TCCGGA               T^CCGGA
MvnI           69  CGCG                 CG^CG
NciI           86  CCGGG                CC^[GC]GG
NsiI          291  ATGCAT               ATGCA^T
PpuMI         387  GGGTCCC              [GA]G^G[AT]CC[CT]
PshAI         312  GACTGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII          62  CAGCTG               CAG^CTG
SacII          68  CCGCGG               CCGC^GG
SfaNI         722  GCATC                GCATC(5/9)
SphI          589  GCATGC               GCATG^C
SspI          958  AATATT               AAT^ATT
StuI          694  AGGCCT               AGG^CCT
XmnI          174  GAACAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccI          367  GTAGAC               GT^[AC][GT]AC
             1291  GTATAC              
AspHI         805  GAGCTC               G[AT]GC[AT]^C
              949  GAGCTC              
AvaII         388  GGTCC                G^G[AT]CC
              795  GGACC               
BamHI         252  GGATCC               G^GATCC
             1102  GGATCC              
BanII         805  GAGCTC               G[GA]GC[CT]^C
              949  GAGCTC              
BbsI          675  GAAGAC               GAAGAC(2/6)
             1204  GAAGAC              
BglII         512  AGATCT               A^GATCT
             1145  AGATCT              
BpuAI         675  GAAGAC               GAAGAC(2/6)
             1204  GAAGAC              
BsaWI         332  ACCGGA               [AT]^CCGG[AT]
             1150  TCCGGA              
BsgI           32  GTGCAG               GTGCAG(16/14)
               45  GTGCAG              
BsiHKAI       805  GAGCTC               G[AT]GC[AT]^C
              949  GAGCTC              
BsmFI         632  GGGAC                GGGAC(10/14)
              389  GTCCC               
BspHI         651  TCATGA               T^CATGA
             1176  TCATGA              
BspMI         403  ACCTGC               ACCTGC(4/8)
              785  ACCTGC              
BstAPI        407  GCAAGCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1123  GCAGATTGTGC         
BstNI         214  CCAGG                CC^[AT]GG
              600  CCTGG               
Cac8I         407  GCAAGC               GC[ACGT]^[ACGT]GC
              589  GCATGC              
DraII         387  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              644  AGGCCCT             
EcoO109I      387  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              644  AGGCCCT             
EcoRII        214  CCAGG                ^CC[AT]GG
              600  CCTGG               
EcoT14I       845  CCAAGG               C^C[AT][AT]GG
              990  CCTAGG              
HaeIII        645  GGCC                 GG^CC
              695  GGCC                
MslI          652  CATGAAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1048  CATGGCAGTG          
MspA1I         62  CAGCTG               C[AC]G^C[GT]G
               68  CCGCGG              
MvaI          214  CCAGG                CC^[AT]GG
              600  CCTGG               
NspI          493  GCATGT               [GA]CATG^[CT]
              589  GCATGC              
PleI          742  GACTC                GAGTC(4/5)
              210  GAGTC               
PstI           80  CTGCAG               CTGCA^G
              314  CTGCAG              
RcaI          651  TCATGA               T^CATGA
             1176  TCATGA              
SacI          805  GAGCTC               GAGCT^C
              949  GAGCTC              
SmlI            4  CTCAAG               C^T[CT][GA]AG
              299  CTCAAG              
StyI          845  CCAAGG               C^C[AT][AT]GG
              990  CCTAGG              
TaqI          227  TCGA                 T^CGA
              713  TCGA                
TfiI          304  GATTC                G^A[AT]TC
              710  GATTC               
TthHB8I       227  TCGA                 T^CGA
              713  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BfaI        BmyI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrDI       BsrI        BssKI       
BstF5I      BstYI       Cfr13I      DdeI        DpnII       
Fnu4HI      FokI        HapII       HinfI       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspI        
MwoI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII       
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