Restriction map for pF1KB0905
 (Circular) (Six-base) MAPSORT of: pF1KB0905.seq  Check: 7585  from: 1  to: 3392



pF1KB0905  3392 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AccI GT'mk_AC

Cuts at:    430     430 
   Size:       3392
 
Acc65I G'GTAC_C

Cuts at:    408     408 
   Size:       3392
 
AclI AA'CG_TT

Cuts at:    386    3232     386 
   Size:       2846     546
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1217    1649    2396    2664    1217 
   Size:        432     747     268    1945

  Fragments arranged by size: 

                1945     747     432     268
 
AflIII A'CryG_T

Cuts at:    744    1848    2825     744 
   Size:       1104     977    1311

  Fragments arranged by size: 

                1311    1104     977
 
AgeI A'CCGG_T

Cuts at:   1780    1780 
   Size:       3392
 
AlwNI CAG_nnn'CTG

Cuts at:    697    2264     697 
   Size:       1567    1825
 
ApaLI G'TGCA_C

Cuts at:   2162    2162 
   Size:       3392
 
ApoI r'AATT_y

Cuts at:    396    2745    3001     396 
   Size:       2349     256     787

  Fragments arranged by size: 

                2349     787     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3392
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3392
 
AvaI C'yCGr_G

Cuts at:    412     412 
   Size:       3392
 
AvrII C'CTAG_G

Cuts at:    159     159 
   Size:       3392
 
BamHI G'GATC_C

Cuts at:    417     417 
   Size:       3392
 
BanI G'GyrC_C

Cuts at:    408     822    1070    1105     408 
   Size:        414     248      35    2695

  Fragments arranged by size: 

                2695     414     248      35
 
BanII G_rGCy'C

Cuts at:    406    1436     406 
   Size:       1030    2362
 
BbeI G_GCGC'C

Cuts at:    826    1074     826 
   Size:        248    3144
 
BbsI GAAGACnn'nnnn_

Cuts at:    294     294 
   Size:       3392
 
BciVI GTATCCnnnnn_n'

Cuts at:   1290    2051    3392    1290 
   Size:        761    1341    1290

  Fragments arranged by size: 

                1341    1290     761
 
BclI T'GATC_A

Cuts at:    912     912 
   Size:       3392
 
BglII A'GATC_T

Cuts at:    113     907    2944     113 
   Size:        794    2037     561

  Fragments arranged by size: 

                2037     794     561
 
BlpI GC'TnA_GC

Cuts at:    504     504 
   Size:       3392
 
Bme1580I G_kGCm'C

Cuts at:   1017    1110    2166    1017 
   Size:         93    1056    2243

  Fragments arranged by size: 

                2243    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    335     604     685    1020    3164     335 
   Size:        269      81     335    2144     563

  Fragments arranged by size: 

                2144     563     335     269      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1759    1759 
   Size:       3392
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    568    1447    1939    2237     568 
   Size:        879     492     298    1723

  Fragments arranged by size: 

                1723     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    262    3096     262 
   Size:       2834     558
 
BsaAI yAC'GTr

Cuts at:    745     745 
   Size:       3392
 
BsaBI GATnn'nnATC

Cuts at:      6     906    2943       6 
   Size:        900    2037     455

  Fragments arranged by size: 

                2037     900     455
 
BsaHI Gr'CG_yC

Cuts at:    823    1071    1773    3269     823 
   Size:        248     702    1496     946

  Fragments arranged by size: 

                1496     946     702     248
 
BsaWI w'CCGG_w

Cuts at:    581    1102    1780    2054    2201    2612     581 
   Size:        521     678     274     147     411    1361

  Fragments arranged by size: 

                1361     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    133     171     133 
   Size:         38    3354
 
BseYI C'CCAG_C

Cuts at:    817    1575    2152    2648     817 
   Size:        758     577     496    1561

  Fragments arranged by size: 

                1561     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     934     980    2188     104 
   Size:        830      46    1208    1308

  Fragments arranged by size: 

                1308    1208     830      46
 
BsiHKAI G_wGCw'C

Cuts at:    286     406    1184    1374    2166     286 
   Size:        120     778     190     792    1512

  Fragments arranged by size: 

                1512     792     778     190     120
 
Bsp1286I G_dGCh'C

Cuts at:    286     406    1017    1110    1184    1374    1436    2166     286 
   Size:        120     611      93      74     190      62     730    1512

  Fragments arranged by size: 

                1512     730     611     190     120      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2612    2612 
   Size:       3392
 
BspHI T'CATG_A

Cuts at:   2568    2568 
   Size:       3392
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    442     656     958    1339     442 
   Size:        214     302     381    2495

  Fragments arranged by size: 

                2495     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1684    1738    3222    3387      50 
   Size:       1634      54    1484     165      55

  Fragments arranged by size: 

                1634    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1304    1304 
   Size:       3392
 
BsrFI r'CCGG_y

Cuts at:   1390    1571    1780    1390 
   Size:        181     209    3002

  Fragments arranged by size: 

                3002     209     181
 
BssSI C'ACGA_G

Cuts at:   1663    2021    1663 
   Size:        358    3034
 
BstAPI GCAn_nnn'nTGC

Cuts at:    769     769 
   Size:       3392
 
BstBI TT'CG_AA

Cuts at:   1753    1753 
   Size:       3392
 
BstYI r'GATC_y

Cuts at:      1     113     236     417     884     907    1242    1488    2500 
   Size:        112     123     181     467      23     335     246    1012

Cuts at:   2500    2586    2598    2944       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     246     181     123
                 112      86      23      12
 
BtgI C'CryG_G

Cuts at:    119     119 
   Size:       3392
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    138    1342    1523    1686     138 
   Size:       1204     181     163    1844

  Fragments arranged by size: 

                1844    1204     181     163
 
BtsI GCAGTG_nn'

Cuts at:    635     635 
   Size:       3392
 
DraI TTT'AAA

Cuts at:    391     391 
   Size:       3392
 
DrdI GACnn_nn'nnGTC

Cuts at:    352    1098    1956     352 
   Size:        746     858    1788

  Fragments arranged by size: 

                1788     858     746
 
EaeI y'GGCC_r

Cuts at:    977    1151    1542    1569    2914     977 
   Size:        174     391      27    1345    1455

  Fragments arranged by size: 

                1455    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:    977     977 
   Size:       3392
 
EarI CTCTTCn'nnn_

Cuts at:   1415    1625    1415 
   Size:        210    3182
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1600    1910    2056    3202    1600 
   Size:        310     146    1146    1790

  Fragments arranged by size: 

                1790    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    404     404 
   Size:       3392
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1217    1649    2396    2664    1217 
   Size:        432     747     268    1945

  Fragments arranged by size: 

                1945     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    190     531     190 
   Size:        341    3051
 
EcoRI G'AATT_C

Cuts at:    396     396 
   Size:       3392
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1411    1411 
   Size:       3392
 
FspI TGC'GCA

Cuts at:   1173    1173 
   Size:       3392
 
HaeII r_GCGC'y

Cuts at:    826    1074    2096    3075     826 
   Size:        248    1022     979    1143

  Fragments arranged by size: 

                1143    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    228     260    1234    1264    1266    1296     228 
   Size:         32     974      30       2      30    2324

  Fragments arranged by size: 

                2324     974      32      30      30       2
 
HincII GTy'rAC

Cuts at:    431     431 
   Size:       3392
 
KasI G'GCGC_C

Cuts at:    822    1070     822 
   Size:        248    3144
 
KpnI G_GTAC'C

Cuts at:    412     412 
   Size:       3392
 
MluI A'CGCG_T

Cuts at:   2825    2825 
   Size:       3392
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2063    2247    2063 
   Size:        184    3208
 
MscI TGG'CCA

Cuts at:   1153    1153 
   Size:       3392
 
MslI CAynn'nnrTG

Cuts at:   1381    1508    1381 
   Size:        127    3265
 
MspA1I CmG'CkG

Cuts at:    249     503     817    1177    2190    2435     249 
   Size:        254     314     360    1013     245    1206

  Fragments arranged by size: 

                1206    1013     360     314     254     245
 
NaeI GCC'GGC

Cuts at:   1573    1573 
   Size:       3392
 
NarI GG'CG_CC

Cuts at:    823    1071     823 
   Size:        248    3144
 
NgoMIV G'CCGG_C

Cuts at:   1571    1571 
   Size:       3392
 
NspI r_CATG'y

Cuts at:    445     945    1852     445 
   Size:        500     907    1985

  Fragments arranged by size: 

                1985     907     500
 
PciI A'CATG_T

Cuts at:   1848    1848 
   Size:       3392
 
PfoI T'CCnGG_A

Cuts at:    356     356 
   Size:       3392
 
PmeI GTTT'AAAC

Cuts at:    391     391 
   Size:       3392
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2531    2563    2531 
   Size:         32    3360
 
PpuMI rG'GwC_Cy

Cuts at:    190     190 
   Size:       3392
 
PstI C_TGCA'G

Cuts at:    439    1124     439 
   Size:        685    2707
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3392
 
PvuII CAG'CTG

Cuts at:    817    1177     817 
   Size:        360    3032
 
RsrII CG'GwC_CG

Cuts at:   1587    1587 
   Size:       3392
 
SacI G_AGCT'C

Cuts at:    406     406 
   Size:       3392
 
SalI G'TCGA_C

Cuts at:    429     429 
   Size:       3392
 
SapI GCTCTTCn'nnn_

Cuts at:   1415    1625    1415 
   Size:        210    3182
 
SbfI CC_TGCA'GG

Cuts at:    439     439 
   Size:       3392
 
ScaI AGT'ACT

Cuts at:   2909    2909 
   Size:       3392
 
SfcI C'TryA_G

Cuts at:     33     435    1120    2113    2304      33 
   Size:        402     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     402     191
 
SfoI GGC'GCC

Cuts at:    824    1072     824 
   Size:        248    3144
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3392
 
SmaI CCC'GGG

Cuts at:    414     414 
   Size:       3392
 
SmlI C'TyrA_G

Cuts at:    547    1462    1954    2216     547 
   Size:        915     492     262    1723

  Fragments arranged by size: 

                1723     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3392
 
SphI G_CATG'C

Cuts at:    445     945     445 
   Size:        500    2892
 
StyI C'CwwG_G

Cuts at:    159     526     880     159 
   Size:        367     354    2671

  Fragments arranged by size: 

                2671     367     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    975    1108    1567    1776     975 
   Size:        133     459     209    2591

  Fragments arranged by size: 

                2591     459     209     133
 
TatI w'GTAC_w

Cuts at:   2907    2907 
   Size:       3392
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2909    2941    2909 
   Size:         32    3360
 
Tth111I GACn'n_nGTC

Cuts at:   1189    1189 
   Size:       3392
 
XbaI T'CTAG_A

Cuts at:    423     423 
   Size:       3392
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    887     887 
   Size:       3392
 
XmaI C'CCGG_G

Cuts at:    412     412 
   Size:       3392
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PciI     PfoI     PmeI     PpiI    PpuMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI     BpmI   Bpu10I    BsaXI     BsgI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     XmnI     ZraI
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