Restriction map for pF1KB0475
 (Linear) (Six-base) MAPSORT of: KIBB0475.seq  Check: 5014  from: 1  to: 1551



KIBB0475.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     520     811    1551 
   Size:        520     291     740

  Fragments arranged by size: 

                 740     520     291
 
AccI GT'mk_AC

Cuts at:      0     362    1551 
   Size:        362    1189
 
Acc65I G'GTAC_C

Cuts at:      0     454     773    1551 
   Size:        454     319     778

  Fragments arranged by size: 

                 778     454     319
 
AflIII A'CryG_T

Cuts at:      0    1181    1551 
   Size:       1181     370
 
AleI CACnn'nnGTG

Cuts at:      0     136    1551 
   Size:        136    1415
 
AlwNI CAG_nnn'CTG

Cuts at:      0     832    1245    1551 
   Size:        832     413     306

  Fragments arranged by size: 

                 832     413     306
 
ApoI r'AATT_y

Cuts at:      0     292    1003    1551 
   Size:        292     711     548

  Fragments arranged by size: 

                 711     548     292
 
AscI GG'CGCG_CC

Cuts at:      0    1041    1551 
   Size:       1041     510
 
BanI G'GyrC_C

Cuts at:      0      22     454     639     773    1053    1139    1551 
   Size:         22     432     185     134     280      86     412

  Fragments arranged by size: 

                 432     412     280     185     134      86      22
 
BbeI G_GCGC'C

Cuts at:      0      26    1143    1551 
   Size:         26    1117     408

  Fragments arranged by size: 

                1117     408      26
 
BbvCI CC'TCA_GC

Cuts at:      0     519    1551 
   Size:        519    1032
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     558    1551 
   Size:        558     993
 
Bme1580I G_kGCm'C

Cuts at:      0     644    1058    1551 
   Size:        644     414     493

  Fragments arranged by size: 

                 644     493     414
 
BmgBI CAC'GTC

Cuts at:      0     138     792    1551 
   Size:        138     654     759

  Fragments arranged by size: 

                 759     654     138
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     533    1300    1551 
   Size:        533     767     251

  Fragments arranged by size: 

                 767     533     251
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     367    1551 
   Size:        361       6    1184

  Fragments arranged by size: 

                1184     361       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     519     975    1475    1551 
   Size:        519     456     500      76

  Fragments arranged by size: 

                 519     500     456      76
 
BsaAI yAC'GTr

Cuts at:      0    1182    1548    1551 
   Size:       1182     366       3

  Fragments arranged by size: 

                1182     366       3
 
BsaHI Gr'CG_yC

Cuts at:      0      23      80    1140    1388    1551 
   Size:         23      57    1060     248     163

  Fragments arranged by size: 

                1060     248     163      57      23
 
BsaWI w'CCGG_w

Cuts at:      0     593    1448    1551 
   Size:        593     855     103

  Fragments arranged by size: 

                 855     593     103
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1005    1035    1551 
   Size:       1005      30     516

  Fragments arranged by size: 

                1005     516      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1013    1324    1327    1330    1333    1336    1339    1342 
   Size:       1013     311       3       3       3       3       3       3

Cuts at:   1342    1551 
   Size:        209

  Fragments arranged by size: 

                1013     311     209       3       3       3       3       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0     172     643     825    1217    1551 
   Size:        172     471     182     392     334

  Fragments arranged by size: 

                 471     392     334     182     172
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     498     748    1551 
   Size:        130     368     250     803

  Fragments arranged by size: 

                 803     368     250     130
 
BsmI GAATG_Cn'

Cuts at:      0      64    1551 
   Size:         64    1487
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1025    1551 
   Size:       1025     526
 
Bsp1286I G_dGCh'C

Cuts at:      0     644    1058    1551 
   Size:        644     414     493

  Fragments arranged by size: 

                 644     493     414
 
BspEI T'CCGG_A

Cuts at:      0     593    1551 
   Size:        593     958
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     520     785     811    1253    1551 
   Size:        520     265      26     442     298

  Fragments arranged by size: 

                 520     442     298     265      26
 
BsrFI r'CCGG_y

Cuts at:      0    1050    1142    1282    1551 
   Size:       1050      92     140     269

  Fragments arranged by size: 

                1050     269     140      92
 
BssHII G'CGCG_C

Cuts at:      0    1041    1551 
   Size:       1041     510
 
BstEII G'GTnAC_C

Cuts at:      0     744    1551 
   Size:        744     807
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     876    1551 
   Size:        876     675
 
BstYI r'GATC_y

Cuts at:      0     590    1551 
   Size:        590     961
 
Bsu36I CC'TnA_GG

Cuts at:      0     740    1551 
   Size:        740     811
 
BtgI C'CryG_G

Cuts at:      0     251    1058    1409    1551 
   Size:        251     807     351     142

  Fragments arranged by size: 

                 807     351     251     142
 
BtsI GCAGTG_nn'

Cuts at:      0     147     470    1551 
   Size:        147     323    1081

  Fragments arranged by size: 

                1081     323     147
 
DraIII CAC_nnn'GTG

Cuts at:      0    1294    1551 
   Size:       1294     257
 
EaeI y'GGCC_r

Cuts at:      0     233     428     506    1061    1551 
   Size:        233     195      78     555     490

  Fragments arranged by size: 

                 555     490     233     195      78
 
EarI CTCTTCn'nnn_

Cuts at:      0     904    1551 
   Size:        904     647
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     316    1551 
   Size:        316    1235
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     367    1551 
   Size:        361       6    1184

  Fragments arranged by size: 

                1184     361       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     498     910     935    1551 
   Size:        498     412      25     616

  Fragments arranged by size: 

                 616     498     412      25
 
EcoRI G'AATT_C

Cuts at:      0     292    1003    1551 
   Size:        292     711     548

  Fragments arranged by size: 

                 711     548     292
 
FseI GG_CCGG'CC

Cuts at:      0    1286    1551 
   Size:       1286     265
 
HaeII r_GCGC'y

Cuts at:      0      26    1143    1551 
   Size:         26    1117     408

  Fragments arranged by size: 

                1117     408      26
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      35      67    1551 
   Size:         35      32    1484

  Fragments arranged by size: 

                1484      35      32
 
HincII GTy'rAC

Cuts at:      0     684    1551 
   Size:        684     867
 
KasI G'GCGC_C

Cuts at:      0      22    1139    1551 
   Size:         22    1117     412

  Fragments arranged by size: 

                1117     412      22
 
KpnI G_GTAC'C

Cuts at:      0     458     777    1551 
   Size:        458     319     774

  Fragments arranged by size: 

                 774     458     319
 
MfeI C'AATT_G

Cuts at:      0     441    1551 
   Size:        441    1110
 
MscI TGG'CCA

Cuts at:      0     235     430     508    1551 
   Size:        235     195      78    1043

  Fragments arranged by size: 

                1043     235     195      78
 
MslI CAynn'nnrTG

Cuts at:      0     136     256     424    1184    1551 
   Size:        136     120     168     760     367

  Fragments arranged by size: 

                 760     367     168     136     120
 
MspA1I CmG'CkG

Cuts at:      0      28     783    1482    1551 
   Size:         28     755     699      69

  Fragments arranged by size: 

                 755     699      69      28
 
NaeI GCC'GGC

Cuts at:      0    1144    1284    1551 
   Size:       1144     140     267

  Fragments arranged by size: 

                1144     267     140
 
NarI GG'CG_CC

Cuts at:      0      23    1140    1551 
   Size:         23    1117     411

  Fragments arranged by size: 

                1117     411      23
 
NcoI C'CATG_G

Cuts at:      0     251    1551 
   Size:        251    1300
 
NgoMIV G'CCGG_C

Cuts at:      0    1142    1282    1551 
   Size:       1142     140     269

  Fragments arranged by size: 

                1142     269     140
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     443    1294    1551 
   Size:        443     851     257

  Fragments arranged by size: 

                 851     443     257
 
PmlI CAC'GTG

Cuts at:      0    1182    1551 
   Size:       1182     369
 
PpuMI rG'GwC_Cy

Cuts at:      0     498     910     935    1551 
   Size:        498     412      25     616

  Fragments arranged by size: 

                 616     498     412      25
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1234    1266    1551 
   Size:       1234      32     285

  Fragments arranged by size: 

                1234     285      32
 
PstI C_TGCA'G

Cuts at:      0     154     782    1250    1551 
   Size:        154     628     468     301

  Fragments arranged by size: 

                 628     468     301     154
 
PvuII CAG'CTG

Cuts at:      0    1482    1551 
   Size:       1482      69
 
SanDI GG'GwC_CC

Cuts at:      0     910    1551 
   Size:        910     641
 
SfcI C'TryA_G

Cuts at:      0     150     778     921    1164    1197    1246    1551 
   Size:        150     628     143     243      33      49     305

  Fragments arranged by size: 

                 628     305     243     150     143      49      33
 
SfoI GGC'GCC

Cuts at:      0      24    1141    1551 
   Size:         24    1117     410

  Fragments arranged by size: 

                1117     410      24
 
StyI C'CwwG_G

Cuts at:      0     251     668     869    1551 
   Size:        251     417     201     682

  Fragments arranged by size: 

                 682     417     251     201
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     202    1241    1282    1551 
   Size:        202    1039      41     269

  Fragments arranged by size: 

                1039     269     202      41
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     649     681    1005    1037    1551 
   Size:        649      32     324      32     514

  Fragments arranged by size: 

                 649     514     324      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     496    1551 
   Size:        496    1055
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     228    1551 
   Size:        228    1323
 

 Enzymes that do cut:

     AarI     AccI   Acc65I   AflIII     AleI    AlwNI     ApoI     AscI
     BanI     BbeI    BbvCI    BciVI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrFI   BssHII   BstEII
    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FseI    HaeII    Hin4I   HincII
     KasI     KpnI     MfeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV    PflMI     PmlI    PpuMI     PsrI     PstI    PvuII
    SanDI     SfcI     SfoI     StyI    TaqII     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AclI     AcuI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI     ApaI    ApaLI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BanII     BbsI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI     BmtI     BplI    BpuEI     BsaI    BsaBI    BsiEI  BsiHKAI
    BsiWI    BspHI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI
  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI  EcoICRI
    EcoNI    EcoRV     FalI     FspI    FspAI  HindIII     HpaI     MluI
     MmeI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     XbaI
     XhoI     XmaI     XmnI     ZraI
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