Restriction map for pF1KB6617
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BsaAI       BsaBI       BsaI        
BseAI       BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        HphI        
KasI        KspI        MaeI        MamI        MfeI        
MluI        MroI        MseI        MunI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       Tsp509I     Tth111I     XbaI        
XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I        340  GGTACC               G^GTACC
AcyI          472  GACGCC               G[GA]^CG[CT]C
AlwI          939  GGATC                GGATC(4/5)
AocI         1161  CCTCAGG              CC^T[ACGT]AGG
Asp718I       340  GGTACC               G^GTACC
BbsI          412  GTCTTC               GAAGAC(2/6)
BbvCI         405  CCTCAGC              CCTCAGC(-5/-2)
BlpI          966  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      966  GCTGAGC              GC^T[ACGT]AGC
BpuAI         412  GTCTTC               GAAGAC(2/6)
BsaHI         472  GACGCC               G[GA]^CG[CT]C
BsaWI         767  ACCGGA               [AT]^CCGG[AT]
BsmBI        1072  GAGACG               CGTCTC(1/5)
BspHI         824  TCATGA               T^CATGA
BsrBI        1209  CCGCTC               CCGCTC(-3/-3)
BssSI        1057  CACGAG               CACGAG(-5/-1)
BstYI         939  GGATCT               [GA]^GATC[CT]
Bsu36I       1161  CCTCAGG              CC^T[ACGT]AGG
CelII         966  GCTGAGC              GC^T[ACGT]AGC
DraIII       1042  CACGTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          102  GAAGAG               CTCTTC(1/4)
Eco81I       1161  CCTCAGG              CC^T[ACGT]AGG
Hin1I         472  GACGCC               G[GA]^CG[CT]C
HincII        577  GTCAAC               GT[CT]^[GA]AC
HindII        577  GTCAAC               GT[CT]^[GA]AC
KpnI          340  GGTACC               GGTAC^C
Ksp632I       102  GAAGAG               CTCTTC(1/4)
MaeII        1043  ACGT                 A^CGT
MaeIII        148  GTCAC                ^GT[ACGT]AC
MflI          939  GGATCT               [GA]^GATC[CT]
NaeI         1124  GCCGGC               GCC^GGC
NcoI          674  CCATGG               C^CATGG
NgoMIV       1124  GCCGGC               G^CCGGC
PaeR7I        481  CTCGAG               C^TCGAG
PvuII          43  CAGCTG               CAG^CTG
RcaI          824  TCATGA               T^CATGA
SapI          102  GAAGAGC              GCTCTTC(1/4)
SmaI          218  CCCGGG               CCC^GGG
Tsp45I        148  GTCAC                ^GT[GC]AC
XhoI          481  CTCGAG               C^TCGAG
XhoII         939  GGATCT               [GA]^GATC[CT]
XmaCI         218  CCCGGG               C^CCGGG
XmaI          218  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AlwNI         859  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              937  CAGGATCTG           
AspHI         105  GAGCTC               G[AT]GC[AT]^C
             1078  GAGCTC              
AvaI          218  CCCGGG               C^[CT]CG[GA]G
              481  CTCGAG              
BalI          875  TGGCCA               TGG^CCA
              890  TGGCCA              
BsgI          982  CTGCAC               GTGCAG(16/14)
              856  GTGCAG              
BsiHKAI       105  GAGCTC               G[AT]GC[AT]^C
             1078  GAGCTC              
BsmAI         787  GAGAC                GTCTC(1/5)
             1072  GAGAC               
BsoBI         218  CCCGGG               C^[CT]CG[GA]G
              481  CTCGAG              
BsrDI          74  CATTGC               GCAATG(2/0)
              389  GCAATG              
BsrFI         722  ACCGGC               [GA]^CCGG[CT]
             1124  GCCGGC              
BsrI           51  CCAGT                ACTGG(1/-1)
              624  CCAGT               
BstAPI        317  GCAAGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              858  GCAGGCGCTGC         
Cfr10I        722  ACCGGC               [GA]^CCGG[CT]
             1124  GCCGGC              
EcoNI         794  CCTTCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              846  CCTGGGGCAGG         
EcoT14I       327  CCTTGG               C^C[AT][AT]GG
              674  CCATGG              
FauI         1026  GCGGG                CCCGC(4/6)
             1035  GCGGG               
HgaI          472  GACGC                GACGC(5/10)
              410  GCGTC               
MluNI         875  TGGCCA               TGG^CCA
              890  TGGCCA              
MscI          875  TGGCCA               TGG^CCA
              890  TGGCCA              
MslI          460  CACAACAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              633  CATGGCCGTG          
MspA1I         43  CAGCTG               C[AC]G^C[GT]G
             1024  CAGCGG              
PflMI         878  CCAAGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              936  CCAGGATCTGG         
PpuMI         779  GGGACCT              [GA]G^G[AT]CC[CT]
             1183  GGGACCC             
SacI          105  GAGCTC               GAGCT^C
             1078  GAGCTC              
SmlI          481  CTCGAG               C^T[CT][GA]AG
              976  CTCAAG              
StyI          327  CCTTGG               C^C[AT][AT]GG
              674  CCATGG              
Van91I        878  CCAAGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              936  CCAGGATCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AvaII       
BanI        BanII       BbvI        BcnI        BglI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmFI       Bsp1286I    BspMI       BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        MboI        MboII       
MnlI        MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        TseI        TspRI       
TthHB8I     XcmI        
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