Restriction map for pF1KB6617
 (Linear) (Six-base) MAPSORT of: KIBB6617.seq  Check: 6671  from: 1  to: 1236



KIBB6617.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      69     340     843    1236 
   Size:         69     271     503     393

  Fragments arranged by size: 

                 503     393     271      69
 
Acc65I G'GTAC_C

Cuts at:      0     340    1236 
   Size:        340     896
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     625    1236 
   Size:        208     417     611

  Fragments arranged by size: 

                 611     417     208
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1036    1236 
   Size:       1036     200
 
AlwNI CAG_nnn'CTG

Cuts at:      0     864     942    1236 
   Size:        864      78     294

  Fragments arranged by size: 

                 864     294      78
 
AvaI C'yCGr_G

Cuts at:      0     218     481    1236 
   Size:        218     263     755

  Fragments arranged by size: 

                 755     263     218
 
BanI G'GyrC_C

Cuts at:      0     340     352    1166    1236 
   Size:        340      12     814      70

  Fragments arranged by size: 

                 814     340      70      12
 
BanII G_rGCy'C

Cuts at:      0      41     109     936    1082    1236 
   Size:         41      68     827     146     154

  Fragments arranged by size: 

                 827     154     146      68      41
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     405    1236 
   Size:        405     831
 
BbvCI CC'TCA_GC

Cuts at:      0     406    1236 
   Size:        406     830
 
BglI GCCn_nnn'nGGC

Cuts at:      0     656     665     898    1097    1236 
   Size:        656       9     233     199     139

  Fragments arranged by size: 

                 656     233     199     139       9
 
BlpI GC'TnA_GC

Cuts at:      0     967    1236 
   Size:        967     269
 
Bme1580I G_kGCm'C

Cuts at:      0     357    1236 
   Size:        357     879
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     123    1236 
   Size:        123    1113
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     118     247     268     448     618     805    1236 
   Size:         25      93     129      21     180     170     187     431

  Fragments arranged by size: 

                 431     187     180     170     129      93      25      21
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     379     406    1236 
   Size:        379      27     830

  Fragments arranged by size: 

                 830     379      27
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     961    1236 
   Size:        961     275
 
BsaHI Gr'CG_yC

Cuts at:      0     473    1236 
   Size:        473     763
 
BsaWI w'CCGG_w

Cuts at:      0     767    1236 
   Size:        767     469
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     356     386    1236 
   Size:        356      30     850

  Fragments arranged by size: 

                 850     356      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     703     964    1084    1236 
   Size:        703     261     120     152

  Fragments arranged by size: 

                 703     261     152     120
 
BseYI C'CCAG_C

Cuts at:      0     135     356     654     867     884    1236 
   Size:        135     221     298     213      17     352

  Fragments arranged by size: 

                 352     298     221     213     135      17
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     877     967    1236 
   Size:        877      90     269

  Fragments arranged by size: 

                 877     269      90
 
BsiHKAI G_wGCw'C

Cuts at:      0     109    1082    1236 
   Size:        109     973     154

  Fragments arranged by size: 

                 973     154     109
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1066    1236 
   Size:       1066     170
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     109     357     936    1082    1236 
   Size:         41      68     248     579     146     154

  Fragments arranged by size: 

                 579     248     154     146      68      41
 
BspHI T'CATG_A

Cuts at:      0     824    1236 
   Size:        824     412
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      69     331     340     843    1236 
   Size:         69     262       9     503     393

  Fragments arranged by size: 

                 503     393     262      69       9
 
BsrBI CCG'CTC

Cuts at:      0    1211    1236 
   Size:       1211      25
 
BsrDI GCAATG_nn'

Cuts at:      0      73     396    1236 
   Size:         73     323     840

  Fragments arranged by size: 

                 840     323      73
 
BsrFI r'CCGG_y

Cuts at:      0     722    1124    1236 
   Size:        722     402     112

  Fragments arranged by size: 

                 722     402     112
 
BssSI C'ACGA_G

Cuts at:      0    1057    1236 
   Size:       1057     179
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     323     864    1236 
   Size:        323     541     372

  Fragments arranged by size: 

                 541     372     323
 
BstYI r'GATC_y

Cuts at:      0     939    1236 
   Size:        939     297
 
Bsu36I CC'TnA_GG

Cuts at:      0    1162    1236 
   Size:       1162      74
 
BtgI C'CryG_G

Cuts at:      0     638     674     774    1236 
   Size:        638      36     100     462

  Fragments arranged by size: 

                 638     462     100      36
 
BtsI GCAGTG_nn'

Cuts at:      0     675     727    1236 
   Size:        675      52     509

  Fragments arranged by size: 

                 675     509      52
 
DraIII CAC_nnn'GTG

Cuts at:      0    1047    1236 
   Size:       1047     189
 
EaeI y'GGCC_r

Cuts at:      0     635     724     875     890     944    1236 
   Size:        635      89     151      15      54     292

  Fragments arranged by size: 

                 635     292     151      89      54      15
 
EarI CTCTTCn'nnn_

Cuts at:      0      97    1236 
   Size:         97    1139
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     801    1236 
   Size:        801     435
 
EcoICRI GAG'CTC

Cuts at:      0     107    1080    1236 
   Size:        107     973     156

  Fragments arranged by size: 

                 973     156     107
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     118     208     247     268     448     618     625 
   Size:         25      93      90      39      21     180     170       7

Cuts at:    625     805    1236 
   Size:        180     431

  Fragments arranged by size: 

                 431     180     180     170      93      90      39      25
                  21       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     798     850    1236 
   Size:        798      52     386

  Fragments arranged by size: 

                 798     386      52
 
EcoO109I rG'GnC_Cy

Cuts at:      0     423     444     780    1134    1184    1236 
   Size:        423      21     336     354      50      52

  Fragments arranged by size: 

                 423     354     336      52      50      21
 
HaeII r_GCGC'y

Cuts at:      0     126     739     865     904    1236 
   Size:        126     613     126      39     332

  Fragments arranged by size: 

                 613     332     126     126      39
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     356     388    1236 
   Size:        356      32     848

  Fragments arranged by size: 

                 848     356      32
 
HincII GTy'rAC

Cuts at:      0     579    1236 
   Size:        579     657
 
KpnI G_GTAC'C

Cuts at:      0     344    1236 
   Size:        344     892
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      80    1236 
   Size:         80    1156
 
MscI TGG'CCA

Cuts at:      0     877     892    1236 
   Size:        877      15     344

  Fragments arranged by size: 

                 877     344      15
 
MslI CAynn'nnrTG

Cuts at:      0     464     637    1236 
   Size:        464     173     599

  Fragments arranged by size: 

                 599     464     173
 
MspA1I CmG'CkG

Cuts at:      0      45    1026    1236 
   Size:         45     981     210

  Fragments arranged by size: 

                 981     210      45
 
NaeI GCC'GGC

Cuts at:      0    1126    1236 
   Size:       1126     110
 
NcoI C'CATG_G

Cuts at:      0     674    1236 
   Size:        674     562
 
NgoMIV G'CCGG_C

Cuts at:      0    1124    1236 
   Size:       1124     112
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     884     942    1236 
   Size:        884      58     294

  Fragments arranged by size: 

                 884     294      58
 
PfoI T'CCnGG_A

Cuts at:      0     245    1236 
   Size:        245     991
 
PpuMI rG'GwC_Cy

Cuts at:      0     780    1184    1236 
   Size:        780     404      52

  Fragments arranged by size: 

                 780     404      52
 
PspXI vC'TCGA_Gb

Cuts at:      0     481    1236 
   Size:        481     755
 
PstI C_TGCA'G

Cuts at:      0     502     713     734    1025    1133    1175    1236 
   Size:        502     211      21     291     108      42      61

  Fragments arranged by size: 

                 502     291     211     108      61      42      21
 
PvuII CAG'CTG

Cuts at:      0      45    1236 
   Size:         45    1191
 
SacI G_AGCT'C

Cuts at:      0     109    1082    1236 
   Size:        109     973     154

  Fragments arranged by size: 

                 973     154     109
 
SanDI GG'GwC_CC

Cuts at:      0    1184    1236 
   Size:       1184      52
 
SapI GCTCTTCn'nnn_

Cuts at:      0      97    1236 
   Size:         97    1139
 
SfcI C'TryA_G

Cuts at:      0     498     709     730    1021    1129    1171    1236 
   Size:        498     211      21     291     108      42      65

  Fragments arranged by size: 

                 498     291     211     108      65      42      21
 
SmaI CCC'GGG

Cuts at:      0     220    1236 
   Size:        220    1016
 
SmlI C'TyrA_G

Cuts at:      0     481     976    1236 
   Size:        481     495     260

  Fragments arranged by size: 

                 495     481     260
 
StyI C'CwwG_G

Cuts at:      0     327     674    1236 
   Size:        327     347     562

  Fragments arranged by size: 

                 562     347     327
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     844     859     876    1236 
   Size:        844      15      17     360

  Fragments arranged by size: 

                 844     360      17      15
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     772     885     900    1236 
   Size:        772     113      15     336

  Fragments arranged by size: 

                 772     336     113      15
 
XhoI C'TCGA_G

Cuts at:      0     481    1236 
   Size:        481     755
 
XmaI C'CCGG_G

Cuts at:      0     218    1236 
   Size:        218    1018
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AlfI    AlwNI     AvaI     BanI    BanII
     BbsI    BbvCI     BglI     BlpI Bme1580I     BplI     BpmI   Bpu10I
    BpuEI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII
     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
    PflMI     PfoI    PpuMI    PspXI     PstI    PvuII     SacI    SanDI
     SapI     SfcI     SmaI     SmlI     StyI    TaqII     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmrI     BmtI     BsaI    BsaAI    BsaBI    BsiEI
    BsiWI     BsmI    BspEI    BsrGI   BssHII    BstBI   BstEII    BstXI
  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI     PsrI     PvuI    RsrII    SacII     SalI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XmnI     ZraI
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