Restriction map for pF1KB0467
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AvaI        AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BciVI       BclI        BcnI        BfrI        BglII       
BlnI        BpmI        BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrFI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HaeII       HapII       
HincII      HindII      HpaI        HpaII       KasI        
KpnI        KspI        MluI        MluNI       MroI        
MscI        MspI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       PstI        PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    StuI        SwaI        TaqI        
Tsp45I      TspRI       TthHB8I     XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AclI         1322  AACGTT               AA^CGTT
AcyI         1316  GACGCC               G[GA]^CG[CT]C
AlwNI         745  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       889  GAACATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1453  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1511  TGCGCA               TGC^GCA
BanI           19  GGCACC               G^G[CT][GA]CC
BcgI          740  CGACACAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          649  GCCCAAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         302  TACGTG               [CT]AC^GT[GA]
BsaBI        1356  GATGCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1316  GACGCC               G[GA]^CG[CT]C
BsmI          702  GAATGC               GAATGC(1/-1)
BspMI        1423  GCAGGT               ACCTGC(4/8)
BsrI         1526  CCAGT                ACTGG(1/-1)
BssSI         937  CTCGTG               CACGAG(-5/-1)
BstXI           9  CCAGCGGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI          70  AGATCC               [GA]^GATC[CT]
CfoI         1512  GCGC                 GCG^C
EcoNI          79  CCTCCTAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         1511  TGCGCA               TGC^GCA
HhaI         1512  GCGC                 GCG^C
Hin1I        1316  GACGCC               G[GA]^CG[CT]C
HinP1I       1512  GCGC                 G^CGC
HindIII      1531  AAGCTT               A^AGCTT
MaeIII        782  GTAAC                ^GT[ACGT]AC
MamI         1356  GATGCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          349  CAATTG               C^AATTG
MflI           70  AGATCC               [GA]^GATC[CT]
MunI          349  CAATTG               C^AATTG
NspI         1519  GCATGC               [GA]CATG^[CT]
PflFI        1453  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         605  CCAAACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1466  AGGTCCT              [GA]G^G[AT]CC[CT]
Psp1406I     1322  AACGTT               AA^CGTT
PvuII         745  CAGCTG               CAG^CTG
SacI          487  GAGCTC               GAGCT^C
SapI          589  GAAGAGC              GCTCTTC(1/4)
SphI         1519  GCATGC               GCATG^C
Tth111I      1453  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        605  CCAAACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          108  TCTAGA               T^CTAGA
XcmI           47  CCACCCCCAAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          70  AGATCC               [GA]^GATC[CT]
XmnI          889  GAACATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcsI         1075  GAATTT               [GA]^AATT[CT]
             1305  GAATTT              
AlwI           71  GATCC                GGATC(4/5)
              177  GGATC               
ApaI           42  GGGCCC               GGGCC^C
              947  GGGCCC              
ApoI         1075  GAATTT               [GA]^AATT[CT]
             1305  GAATTT              
AspHI         100  GAGCAC               G[AT]GC[AT]^C
              487  GAGCTC              
BbsI          619  GAAGAC               GAAGAC(2/6)
             1096  GAAGAC              
BlpI           97  GCTGAGC              GC^T[ACGT]AGC
              567  GCTGAGC             
Bpu1102I       97  GCTGAGC              GC^T[ACGT]AGC
              567  GCTGAGC             
BpuAI         619  GAAGAC               GAAGAC(2/6)
             1096  GAAGAC              
BsiHKAI       100  GAGCAC               G[AT]GC[AT]^C
              487  GAGCTC              
BsmBI         911  GAGACG               CGTCTC(1/5)
             1044  GAGACG              
BsmFI         197  GGGAC                GGGAC(10/14)
             1380  GTCCC               
Bsp1407I        2  TGTACA               T^GTACA
             1183  TGTACA              
BsrBI         172  GAGCGG               CCGCTC(-3/-3)
              535  GAGCGG              
BsrGI           2  TGTACA               T^GTACA
             1183  TGTACA              
BstAPI        544  GCACAGATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1514  GCAAGGCATGC         
CelII          97  GCTGAGC              GC^T[ACGT]AGC
              567  GCTGAGC             
EcoT14I        90  CCAAGG               C^C[AT][AT]GG
              632  CCAAGG              
FauI           30  GCGGG                CCCGC(4/6)
              174  GCGGG               
HgaI          122  GACGC                GACGC(5/10)
             1316  GACGC               
HinfI         116  GAATC                G^A[ACGT]TC
              245  GAATC               
HphI           60  GGTGA                GGTGA(8/7)
              713  TCACC               
MslI          802  CATGAGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1261  CACATAAATG          
SspBI           2  TGTACA               T^GTACA
             1183  TGTACA              
SspI         1031  AATATT               AAT^ATT
             1086  AATATT              
StyI           90  CCAAGG               C^C[AT][AT]GG
              632  CCAAGG              
TfiI          116  GAATC                G^A[AT]TC
              245  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BanII       
BbvI        BfaI        BmyI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       Bsp1286I    BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EarI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      ItaI        Ksp632I     
MaeI        MaeII       MboI        MboII       MnlI        
MseI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       Tru9I       TseI        Tsp509I     
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