Restriction map for pF1KB0467
 (Linear) (Six-base) MAPSORT of: KIBB0467.seq  Check: 2618  from: 1  to: 1542



KIBB0467.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1414    1542 
   Size:       1414     128
 
AclI AA'CG_TT

Cuts at:      0    1323    1542 
   Size:       1323     219
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     772    1336    1542 
   Size:        772     564     206

  Fragments arranged by size: 

                 772     564     206
 
AlwNI CAG_nnn'CTG

Cuts at:      0     750    1542 
   Size:        750     792
 
ApaI G_GGCC'C

Cuts at:      0      46     951    1542 
   Size:         46     905     591

  Fragments arranged by size: 

                 905     591      46
 
ApoI r'AATT_y

Cuts at:      0    1075    1305    1542 
   Size:       1075     230     237

  Fragments arranged by size: 

                1075     237     230
 
BanI G'GyrC_C

Cuts at:      0      19    1542 
   Size:         19    1523
 
BanII G_rGCy'C

Cuts at:      0      46     491     951    1542 
   Size:         46     445     460     591

  Fragments arranged by size: 

                 591     460     445      46
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     626    1103    1542 
   Size:        626     477     439

  Fragments arranged by size: 

                 626     477     439
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     729     763    1542 
   Size:        729      34     779

  Fragments arranged by size: 

                 779     729      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     655    1542 
   Size:        655     887
 
BlpI GC'TnA_GC

Cuts at:      0      98     568    1542 
   Size:         98     470     974

  Fragments arranged by size: 

                 974     470      98
 
Bme1580I G_kGCm'C

Cuts at:      0      46     951    1542 
   Size:         46     905     591

  Fragments arranged by size: 

                 905     591      46
 
BmgBI CAC'GTC

Cuts at:      0    1048    1542 
   Size:       1048     494
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1520    1542 
   Size:       1520      22
 
Bpu10I CC'TnA_GC

Cuts at:      0    1064    1542 
   Size:       1064     478
 
BsaAI yAC'GTr

Cuts at:      0     304    1542 
   Size:        304    1238
 
BsaBI GATnn'nnATC

Cuts at:      0    1360    1542 
   Size:       1360     182
 
BsaHI Gr'CG_yC

Cuts at:      0    1317    1542 
   Size:       1317     225
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1456    1486    1542 
   Size:       1456      30      56

  Fragments arranged by size: 

                1456      56      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     385     499     547     577     675     775    1542 
   Size:        385     114      48      30      98     100     767

  Fragments arranged by size: 

                 767     385     114     100      98      48      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     104     491    1542 
   Size:        104     387    1051

  Fragments arranged by size: 

                1051     387     104
 
BsmI GAATG_Cn'

Cuts at:      0     708    1542 
   Size:        708     834
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     905    1038    1542 
   Size:        905     133     504

  Fragments arranged by size: 

                 905     504     133
 
Bsp1286I G_dGCh'C

Cuts at:      0      46     104     491     951    1542 
   Size:         46      58     387     460     591

  Fragments arranged by size: 

                 591     460     387      58      46
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1414    1542 
   Size:       1414     128
 
BsrBI CCG'CTC

Cuts at:      0     174     537    1542 
   Size:        174     363    1005

  Fragments arranged by size: 

                1005     363     174
 
BsrGI T'GTAC_A

Cuts at:      0       2    1183    1542 
   Size:          2    1181     359

  Fragments arranged by size: 

                1181     359       2
 
BssSI C'ACGA_G

Cuts at:      0     937    1542 
   Size:        937     605
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     550    1520    1542 
   Size:        550     970      22

  Fragments arranged by size: 

                 970     550      22
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      16    1542 
   Size:         16    1526
 
BstYI r'GATC_y

Cuts at:      0      70    1542 
   Size:         70    1472
 
EarI CTCTTCn'nnn_

Cuts at:      0     584     752     962    1289    1394    1542 
   Size:        584     168     210     327     105     148

  Fragments arranged by size: 

                 584     327     210     168     148     105
 
EcoICRI GAG'CTC

Cuts at:      0     489    1542 
   Size:        489    1053
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     772    1336    1542 
   Size:        772     564     206

  Fragments arranged by size: 

                 772     564     206
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      83    1542 
   Size:         83    1459
 
EcoO109I rG'GnC_Cy

Cuts at:      0      42      43     947     948    1467    1542 
   Size:         42       1     904       1     519      75

  Fragments arranged by size: 

                 904     519      75      42       1       1
 
FspI TGC'GCA

Cuts at:      0    1513    1542 
   Size:       1513      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     230     262    1456    1488    1542 
   Size:        230      32    1194      32      54

  Fragments arranged by size: 

                1194     230      54      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1531    1542 
   Size:       1531      11
 
MfeI C'AATT_G

Cuts at:      0     349    1542 
   Size:        349    1193
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     144    1542 
   Size:        144    1398
 
MslI CAynn'nnrTG

Cuts at:      0     806    1265    1542 
   Size:        806     459     277

  Fragments arranged by size: 

                 806     459     277
 
MspA1I CmG'CkG

Cuts at:      0      12      30     747    1542 
   Size:         12      18     717     795

  Fragments arranged by size: 

                 795     717      18      12
 
NspI r_CATG'y

Cuts at:      0    1523    1542 
   Size:       1523      19
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     611    1542 
   Size:        611     931
 
PfoI T'CCnGG_A

Cuts at:      0    1460    1542 
   Size:       1460      82
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     583     615    1542 
   Size:        583      32     927

  Fragments arranged by size: 

                 927     583      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1467    1542 
   Size:       1467      75
 
PspOMI G'GGCC_C

Cuts at:      0      42     947    1542 
   Size:         42     905     595

  Fragments arranged by size: 

                 905     595      42
 
PvuII CAG'CTG

Cuts at:      0     747    1542 
   Size:        747     795
 
SacI G_AGCT'C

Cuts at:      0     491    1542 
   Size:        491    1051
 
SapI GCTCTTCn'nnn_

Cuts at:      0     584    1542 
   Size:        584     958
 
SphI G_CATG'C

Cuts at:      0    1523    1542 
   Size:       1523      19
 
SspI AAT'ATT

Cuts at:      0    1033    1088    1542 
   Size:       1033      55     454

  Fragments arranged by size: 

                1033     454      55
 
StyI C'CwwG_G

Cuts at:      0      90     632    1542 
   Size:         90     542     910

  Fragments arranged by size: 

                 910     542      90
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     638    1492    1542 
   Size:        638     854      50

  Fragments arranged by size: 

                 854     638      50
 
TatI w'GTAC_w

Cuts at:      0       2    1183    1337    1542 
   Size:          2    1181     154     205

  Fragments arranged by size: 

                1181     205     154       2
 
Tth111I GACn'n_nGTC

Cuts at:      0    1456    1542 
   Size:       1456      86
 
XbaI T'CTAG_A

Cuts at:      0     108    1542 
   Size:        108    1434
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      54    1542 
   Size:         54    1488
 
XmnI GAAnn'nnTTC

Cuts at:      0     893    1542 
   Size:        893     649
 

 Enzymes that do cut:

     AarI     AclI     AcuI    AlwNI     ApaI     ApoI     BanI    BanII
     BbsI     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI   Bpu10I
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrGI    BssSI   BstAPI    BstXI    BstYI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    Hin4I  HindIII
     MfeI     MmeI     MslI   MspA1I     NspI    PflMI     PfoI     PpiI
    PpuMI   PspOMI    PvuII     SacI     SapI     SphI     SspI     StyI
    TaqII     TatI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI    BciVI     BclI    BfrBI
    BglII     BmtI     BplI     BpmI    BpuEI     BsaI    BsaWI    BseYI
     BsgI    BsiEI    BsiWI    BspEI    BspHI    BsrDI    BsrFI   BssHII
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoRI
    EcoRV     FalI     FseI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PmlI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     StuI     SwaI     TstI     XhoI     XmaI     ZraI
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