Restriction map for pF1KB0463
 (Linear) (Six-base) MAPSORT of: KIBB0463.seq  Check: 4544  from: 1  to: 1527



KIBB0463.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     509    1527 
   Size:        509    1018
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     751     799    1215    1527 
   Size:        751      48     416     312

  Fragments arranged by size: 

                 751     416     312      48
 
AflII C'TTAA_G

Cuts at:      0    1395    1527 
   Size:       1395     132
 
AlwNI CAG_nnn'CTG

Cuts at:      0     567     834     861    1527 
   Size:        567     267      27     666

  Fragments arranged by size: 

                 666     567     267      27
 
ApaI G_GGCC'C

Cuts at:      0     246    1340    1527 
   Size:        246    1094     187

  Fragments arranged by size: 

                1094     246     187
 
AvaI C'yCGr_G

Cuts at:      0     670     706    1412    1527 
   Size:        670      36     706     115

  Fragments arranged by size: 

                 706     670     115      36
 
BanI G'GyrC_C

Cuts at:      0     223     436     465     508    1043    1527 
   Size:        223     213      29      43     535     484

  Fragments arranged by size: 

                 535     484     223     213      43      29
 
BanII G_rGCy'C

Cuts at:      0     246     679    1229    1340    1527 
   Size:        246     433     550     111     187

  Fragments arranged by size: 

                 550     433     246     187     111
 
BbeI G_GCGC'C

Cuts at:      0     440     512    1047    1527 
   Size:        440      72     535     480

  Fragments arranged by size: 

                 535     480     440      72
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     996    1338    1527 
   Size:        996     342     189

  Fragments arranged by size: 

                 996     342     189
 
BbvCI CC'TCA_GC

Cuts at:      0      47    1527 
   Size:         47    1480
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     537     571     697     731    1527 
   Size:        537      34     126      34     796

  Fragments arranged by size: 

                 796     537     126      34      34
 
BclI T'GATC_A

Cuts at:      0     863    1527 
   Size:        863     664
 
BglI GCCn_nnn'nGGC

Cuts at:      0     377     743    1073    1527 
   Size:        377     366     330     454

  Fragments arranged by size: 

                 454     377     366     330
 
BglII A'GATC_T

Cuts at:      0    1433    1527 
   Size:       1433      94
 
BlpI GC'TnA_GC

Cuts at:      0     773     886    1527 
   Size:        773     113     641

  Fragments arranged by size: 

                 773     641     113
 
Bme1580I G_kGCm'C

Cuts at:      0     246     470     592    1340    1527 
   Size:        246     224     122     748     187

  Fragments arranged by size: 

                 748     246     224     187     122
 
BmgBI CAC'GTC

Cuts at:      0     176    1527 
   Size:        176    1351
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      71    1527 
   Size:         71    1456
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     782     811     820    1141    1527 
   Size:        203     579      29       9     321     386

  Fragments arranged by size: 

                 579     386     321     203      29       9
 
Bpu10I CC'TnA_GC

Cuts at:      0      47    1527 
   Size:         47    1480
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1519    1527 
   Size:       1519       8
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1020    1527 
   Size:       1020     507
 
BsaAI yAC'GTr

Cuts at:      0     477    1527 
   Size:        477    1050
 
BsaHI Gr'CG_yC

Cuts at:      0     437     509    1044    1527 
   Size:        437      72     535     483

  Fragments arranged by size: 

                 535     483     437      72
 
BsaWI w'CCGG_w

Cuts at:      0    1451    1527 
   Size:       1451      76
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     623     653     974    1004    1527 
   Size:        623      30     321      30     523

  Fragments arranged by size: 

                 623     523     321      30      30
 
BseYI C'CCAG_C

Cuts at:      0     180     205     360     567     746    1371    1486    1527 
   Size:        180      25     155     207     179     625     115      41

  Fragments arranged by size: 

                 625     207     180     179     155     115      41      25
            
 
BsiEI CG_ry'CG

Cuts at:      0     875    1527 
   Size:        875     652
 
BsiHKAI G_wGCw'C

Cuts at:      0    1229    1527 
   Size:       1229     298
 
BsmI GAATG_Cn'

Cuts at:      0     548    1527 
   Size:        548     979
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     166    1152    1527 
   Size:        166     986     375

  Fragments arranged by size: 

                 986     375     166
 
Bsp1286I G_dGCh'C

Cuts at:      0     246     470     592     679    1229    1340    1527 
   Size:        246     224     122      87     550     111     187

  Fragments arranged by size: 

                 550     246     224     187     122     111      87
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     509     642    1527 
   Size:        509     133     885

  Fragments arranged by size: 

                 885     509     133
 
BsrBI CCG'CTC

Cuts at:      0     810    1035    1527 
   Size:        810     225     492

  Fragments arranged by size: 

                 810     492     225
 
BsrDI GCAATG_nn'

Cuts at:      0     866    1527 
   Size:        866     661
 
BsrFI r'CCGG_y

Cuts at:      0     851    1527 
   Size:        851     676
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     477    1263    1527 
   Size:        477     786     264

  Fragments arranged by size: 

                 786     477     264
 
BstYI r'GATC_y

Cuts at:      0    1433    1527 
   Size:       1433      94
 
Bsu36I CC'TnA_GG

Cuts at:      0     197    1527 
   Size:        197    1330
 
BtgI C'CryG_G

Cuts at:      0     251     266    1256    1527 
   Size:        251      15     990     271

  Fragments arranged by size: 

                 990     271     251      15
 
BtsI GCAGTG_nn'

Cuts at:      0     590     607    1527 
   Size:        590      17     920

  Fragments arranged by size: 

                 920     590      17
 
EaeI y'GGCC_r

Cuts at:      0     254     383     533     604     811    1157    1220    1527 
   Size:        254     129     150      71     207     346      63     307

  Fragments arranged by size: 

                 346     307     254     207     150     129      71      63
            
 
EarI CTCTTCn'nnn_

Cuts at:      0    1234    1527 
   Size:       1234     293
 
EcoICRI GAG'CTC

Cuts at:      0    1227    1527 
   Size:       1227     300
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     751     782     799     811     820    1141    1215 
   Size:        203     548      31      17      12       9     321      74

Cuts at:   1215    1527 
   Size:        312

  Fragments arranged by size: 

                 548     321     312     203      74      31      17      12
                   9
 
EcoO109I rG'GnC_Cy

Cuts at:      0      65     193     202     243     712    1240    1336    1337 
   Size:         65     128       9      41     469     528      96       1

Cuts at:   1337    1527 
   Size:        190

  Fragments arranged by size: 

                 528     469     190     128      96      65      41       9
                   1
 
HaeII r_GCGC'y

Cuts at:      0     440     512     738    1047    1527 
   Size:        440      72     226     309     480

  Fragments arranged by size: 

                 480     440     309     226      72
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     413     445    1041    1073    1092    1124    1420    1446 
   Size:        413      32     596      32      19      32     296      26

Cuts at:   1446    1452    1478    1527 
   Size:          6      26      49

  Fragments arranged by size: 

                 596     413     296      49      32      32      32      26
                  26      19       6
 
HincII GTy'rAC

Cuts at:      0     342     996    1527 
   Size:        342     654     531

  Fragments arranged by size: 

                 654     531     342
 
KasI G'GCGC_C

Cuts at:      0     436     508    1043    1527 
   Size:        436      72     535     484

  Fragments arranged by size: 

                 535     484     436      72
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      39    1301    1527 
   Size:         39    1262     226

  Fragments arranged by size: 

                1262     226      39
 
MscI TGG'CCA

Cuts at:      0     256     385     535     606    1159    1527 
   Size:        256     129     150      71     553     368

  Fragments arranged by size: 

                 553     368     256     150     129      71
 
MslI CAynn'nnrTG

Cuts at:      0     475    1096    1261    1303    1527 
   Size:        475     621     165      42     224

  Fragments arranged by size: 

                 621     475     224     165      42
 
MspA1I CmG'CkG

Cuts at:      0     648     885    1441    1527 
   Size:        648     237     556      86

  Fragments arranged by size: 

                 648     556     237      86
 
NarI GG'CG_CC

Cuts at:      0     437     509    1044    1527 
   Size:        437      72     535     483

  Fragments arranged by size: 

                 535     483     437      72
 
NcoI C'CATG_G

Cuts at:      0    1256    1527 
   Size:       1256     271
 
NspI r_CATG'y

Cuts at:      0    1080    1527 
   Size:       1080     447
 
PasI CC'CwG_GG

Cuts at:      0     513    1527 
   Size:        513    1014
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     239     567     602     834    1062    1208    1527 
   Size:        239     328      35     232     228     146     319

  Fragments arranged by size: 

                 328     319     239     232     228     146      35
 
PpuMI rG'GwC_Cy

Cuts at:      0      65     202    1240    1527 
   Size:         65     137    1038     287

  Fragments arranged by size: 

                1038     287     137      65
 
PshAI GACnn'nnGTC

Cuts at:      0      70    1527 
   Size:         70    1457
 
PspOMI G'GGCC_C

Cuts at:      0     242    1336    1527 
   Size:        242    1094     191

  Fragments arranged by size: 

                1094     242     191
 
PspXI vC'TCGA_Gb

Cuts at:      0    1412    1527 
   Size:       1412     115
 
PstI C_TGCA'G

Cuts at:      0     110     647     884    1527 
   Size:        110     537     237     643

  Fragments arranged by size: 

                 643     537     237     110
 
PvuII CAG'CTG

Cuts at:      0     648     885    1441    1527 
   Size:        648     237     556      86

  Fragments arranged by size: 

                 648     556     237      86
 
SacI G_AGCT'C

Cuts at:      0    1229    1527 
   Size:       1229     298
 
SanDI GG'GwC_CC

Cuts at:      0      65     202    1240    1527 
   Size:         65     137    1038     287

  Fragments arranged by size: 

                1038     287     137      65
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1234    1527 
   Size:       1234     293
 
SfcI C'TryA_G

Cuts at:      0     106     643     880    1527 
   Size:        106     537     237     647

  Fragments arranged by size: 

                 647     537     237     106
 
SfoI GGC'GCC

Cuts at:      0     438     510    1045    1527 
   Size:        438      72     535     482

  Fragments arranged by size: 

                 535     482     438      72
 
SmlI C'TyrA_G

Cuts at:      0     706    1395    1412    1498    1527 
   Size:        706     689      17      86      29

  Fragments arranged by size: 

                 706     689      86      29      17
 
StyI C'CwwG_G

Cuts at:      0     417    1010    1207    1256    1527 
   Size:        417     593     197      49     271

  Fragments arranged by size: 

                 593     417     271     197      49
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     974    1006    1527 
   Size:        974      32     521

  Fragments arranged by size: 

                 974     521      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     264     898    1527 
   Size:        264     634     629

  Fragments arranged by size: 

                 634     629     264
 
XhoI C'TCGA_G

Cuts at:      0     706    1412    1527 
   Size:        706     706     115

  Fragments arranged by size: 

                 706     706     115
 

 Enzymes that do cut:

     AarI     AcuI    AflII    AlwNI     ApaI     AvaI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BstXI    BstYI   Bsu36I
     BtgI     BtsI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    HaeII
    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NspI     PasI    PflMI    PpuMI    PshAI   PspOMI    PspXI
     PstI    PvuII     SacI    SanDI     SapI     SfcI     SfoI     SmlI
     StyI     TstI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BciVI    BfrBI     BmtI     BplI    BsaBI
    BseRI     BsgI    BsiWI    BspEI    BspHI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI     PsiI     PsrI     PvuI
    RsrII    SacII     SalI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI  Tth111I     XbaI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com