Restriction map for pF1KB0449
 (Linear) (Six-base) MAPSORT of: KIBB0449.seq  Check: 8322  from: 1  to: 1422



KIBB0449.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AatII G_ACGT'C

Cuts at:      0     278    1422 
   Size:        278    1144
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601     610     697     817     856    1351    1422 
   Size:        601       9      87     120      39     495      71

  Fragments arranged by size: 

                 601     495     120      87      71      39       9
 
AleI CACnn'nnGTG

Cuts at:      0     649    1422 
   Size:        649     773
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     799    1422 
   Size:        799     623
 
AlwNI CAG_nnn'CTG

Cuts at:      0     687     810    1252    1422 
   Size:        687     123     442     170

  Fragments arranged by size: 

                 687     442     170     123
 
ApaLI G'TGCA_C

Cuts at:      0    1294    1422 
   Size:       1294     128
 
ApoI r'AATT_y

Cuts at:      0      85     322    1422 
   Size:         85     237    1100

  Fragments arranged by size: 

                1100     237      85
 
AvaI C'yCGr_G

Cuts at:      0    1023    1069    1422 
   Size:       1023      46     353

  Fragments arranged by size: 

                1023     353      46
 
BamHI G'GATC_C

Cuts at:      0     940    1422 
   Size:        940     482
 
BanI G'GyrC_C

Cuts at:      0     457    1422 
   Size:        457     965
 
BanII G_rGCy'C

Cuts at:      0     352    1030    1422 
   Size:        352     678     392

  Fragments arranged by size: 

                 678     392     352
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     465    1313    1422 
   Size:        465     848     109

  Fragments arranged by size: 

                 848     465     109
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1176    1422 
   Size:       1176     246
 
BclI T'GATC_A

Cuts at:      0       2    1422 
   Size:          2    1420
 
BglII A'GATC_T

Cuts at:      0     354    1422 
   Size:        354    1068
 
BlpI GC'TnA_GC

Cuts at:      0     370    1422 
   Size:        370    1052
 
Bme1580I G_kGCm'C

Cuts at:      0      53     460     878    1298    1422 
   Size:         53     407     418     420     124

  Fragments arranged by size: 

                 420     418     407     124      53
 
BmgBI CAC'GTC

Cuts at:      0    1091    1422 
   Size:       1091     331
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     457    1422 
   Size:        457     965
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     308    1422 
   Size:        308    1114
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550    1330    1422 
   Size:        550     780      92

  Fragments arranged by size: 

                 780     550      92
 
Bpu10I CC'TnA_GC

Cuts at:      0    1204    1422 
   Size:       1204     218
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      55     149    1283    1422 
   Size:         55      94    1134     139

  Fragments arranged by size: 

                1134     139      94      55
 
BsaAI yAC'GTr

Cuts at:      0     432    1422 
   Size:        432     990
 
BsaHI Gr'CG_yC

Cuts at:      0     275    1422 
   Size:        275    1147
 
BsaWI w'CCGG_w

Cuts at:      0    1274    1422 
   Size:       1274     148
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1277    1300    1307    1330    1422 
   Size:       1277      23       7      23      92

  Fragments arranged by size: 

                1277      92      23      23       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     217     553    1057    1422 
   Size:        217     336     504     365

  Fragments arranged by size: 

                 504     365     336     217
 
BseYI C'CCAG_C

Cuts at:      0     936    1422 
   Size:        936     486
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     250     537     769    1033    1422 
   Size:        192      58     287     232     264     389

  Fragments arranged by size: 

                 389     287     264     232     192      58
 
BsiHKAI G_wGCw'C

Cuts at:      0     536    1298    1422 
   Size:        536     762     124

  Fragments arranged by size: 

                 762     536     124
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1081    1422 
   Size:       1081     341
 
Bsp1286I G_dGCh'C

Cuts at:      0      53     352     460     536     878    1030    1298    1422 
   Size:         53     299     108      76     342     152     268     124

  Fragments arranged by size: 

                 342     299     268     152     124     108      76      53
            
 
BspHI T'CATG_A

Cuts at:      0       5     707    1422 
   Size:          5     702     715

  Fragments arranged by size: 

                 715     702       5
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     818    1422 
   Size:        818     604
 
BsrBI CCG'CTC

Cuts at:      0     369    1158    1422 
   Size:        369     789     264

  Fragments arranged by size: 

                 789     369     264
 
BsrFI r'CCGG_y

Cuts at:      0     116    1422 
   Size:        116    1306
 
BssSI C'ACGA_G

Cuts at:      0     535    1422 
   Size:        535     887
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1252    1422 
   Size:       1252     170
 
BstEII G'GTnAC_C

Cuts at:      0     903    1422 
   Size:        903     519
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     900    1422 
   Size:        900     522
 
BstYI r'GATC_y

Cuts at:      0     354     521     779     940    1337    1422 
   Size:        354     167     258     161     397      85

  Fragments arranged by size: 

                 397     354     258     167     161      85
 
BtgI C'CryG_G

Cuts at:      0     266     299    1422 
   Size:        266      33    1123

  Fragments arranged by size: 

                1123     266      33
 
BtsI GCAGTG_nn'

Cuts at:      0     554    1310    1422 
   Size:        554     756     112

  Fragments arranged by size: 

                 756     554     112
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     945     980    1422 
   Size:        945      35     442

  Fragments arranged by size: 

                 945     442      35
 
EaeI y'GGCC_r

Cuts at:      0     842     857    1001    1422 
   Size:        842      15     144     421

  Fragments arranged by size: 

                 842     421     144      15
 
EarI CTCTTCn'nnn_

Cuts at:      0    1024    1079    1422 
   Size:       1024      55     343

  Fragments arranged by size: 

                1024     343      55
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     135     298    1422 
   Size:        135     163    1124

  Fragments arranged by size: 

                1124     163     135
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550     601     610     697     817     856    1330    1351 
   Size:        550      51       9      87     120      39     474      21

Cuts at:   1351    1422 
   Size:         71

  Fragments arranged by size: 

                 550     474     120      87      71      51      39      21
                   9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     316    1422 
   Size:        316    1106
 
EcoO109I rG'GnC_Cy

Cuts at:      0     753     792     920    1013    1073    1353    1422 
   Size:        753      39     128      93      60     280      69

  Fragments arranged by size: 

                 753     280     128      93      69      60      39
 
EcoRI G'AATT_C

Cuts at:      0      85     322    1422 
   Size:         85     237    1100

  Fragments arranged by size: 

                1100     237      85
 
EcoRV GAT'ATC

Cuts at:      0     306    1422 
   Size:        306    1116
 
HaeII r_GCGC'y

Cuts at:      0      33     258     550    1422 
   Size:         33     225     292     872

  Fragments arranged by size: 

                 872     292     225      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     339     371    1300    1332    1422 
   Size:        339      32     929      32      90

  Fragments arranged by size: 

                 929     339      90      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1160    1422 
   Size:       1160     262
 
MscI TGG'CCA

Cuts at:      0     859    1003    1422 
   Size:        859     144     419

  Fragments arranged by size: 

                 859     419     144
 
MslI CAynn'nnrTG

Cuts at:      0     649     712     967    1422 
   Size:        649      63     255     455

  Fragments arranged by size: 

                 649     455     255      63
 
MspA1I CmG'CkG

Cuts at:      0     197     240     744    1422 
   Size:        197      43     504     678

  Fragments arranged by size: 

                 678     504     197      43
 
NcoI C'CATG_G

Cuts at:      0     266     299    1422 
   Size:        266      33    1123

  Fragments arranged by size: 

                1123     266      33
 
NspI r_CATG'y

Cuts at:      0     381     717    1242    1422 
   Size:        381     336     525     180

  Fragments arranged by size: 

                 525     381     336     180
 
PpuMI rG'GwC_Cy

Cuts at:      0     792    1073    1353    1422 
   Size:        792     281     280      69

  Fragments arranged by size: 

                 792     281     280      69
 
PvuII CAG'CTG

Cuts at:      0     744    1422 
   Size:        744     678
 
SanDI GG'GwC_CC

Cuts at:      0    1073    1422 
   Size:       1073     349
 
ScaI AGT'ACT

Cuts at:      0     952    1422 
   Size:        952     470
 
SfcI C'TryA_G

Cuts at:      0     328     337    1128    1422 
   Size:        328       9     791     294

  Fragments arranged by size: 

                 791     328     294       9
 
SmaI CCC'GGG

Cuts at:      0    1025    1422 
   Size:       1025     397
 
SphI G_CATG'C

Cuts at:      0     717    1422 
   Size:        717     705
 
SspI AAT'ATT

Cuts at:      0     603    1422 
   Size:        603     819
 
StyI C'CwwG_G

Cuts at:      0      77     266     299    1422 
   Size:         77     189      33    1123

  Fragments arranged by size: 

                1123     189      77      33
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     130     626    1422 
   Size:        130     496     796

  Fragments arranged by size: 

                 796     496     130
 
TatI w'GTAC_w

Cuts at:      0     950    1422 
   Size:        950     472
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1277    1309    1422 
   Size:       1277      32     113

  Fragments arranged by size: 

                1277     113      32
 
XmaI C'CCGG_G

Cuts at:      0    1023    1422 
   Size:       1023     399
 
ZraI GAC'GTC

Cuts at:      0     276    1422 
   Size:        276    1146
 

 Enzymes that do cut:

    AatII     AcuI     AleI     AlfI    AlwNI    ApaLI     ApoI     AvaI
    BamHI     BanI    BanII     BbsI    BciVI     BclI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BssSI   BstAPI   BstEII
    BstXI    BstYI     BtgI     BtsI    CspCI     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV    HaeII    Hin4I     MmeI
     MscI     MslI   MspA1I     NcoI     NspI    PpuMI    PvuII    SanDI
     ScaI     SfcI     SmaI     SphI     SspI     StyI    TaqII     TatI
     TstI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI    BbvCI     BcgI    BfrBI     BglI     BmtI    BpuEI    BsaBI
    BsiEI    BsiWI     BsmI    BspEI    BsrDI    BsrGI   BssHII    BstBI
  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII     DrdI     EagI
  EcoICRI     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SrfI     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com