Restriction map for pF1KB0447
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AocI        
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BbrPI       BbvCI       
BcgI        BclI        BfrI        BlnI        BsaAI       
BsaBI       BseAI       BsgI        BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrGI       Bst1107I    BstBI       BstXI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoNI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluNI       MroI        MscI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        SexAI       SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AflIII        534  ACGCGT               A^C[GA][CT]GT
AlwNI         211  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI          746  CCCGGG               C^[CT]CG[GA]G
BbeI           60  GGCGCC               GGCGC^C
BciVI         759  GGATAC               GTATCC(6/5)
BfaI          376  CTAG                 C^TAG
BglI          557  GCCTGGTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1076  AGATCT               A^GATCT
BsiEI         651  CGACCG               CG[GA][CT]^CG
BsiWI         857  CGTACG               C^GTACG
BsoBI         746  CCCGGG               C^[CT]CG[GA]G
BsrDI         461  GCAATG               GCAATG(2/0)
BsrFI         653  ACCGGC               [GA]^CCGG[CT]
BssHII        100  GCGCGC               G^CGCGC
BssSI         910  CACGAG               CACGAG(-5/-1)
BstAPI        108  GCACAGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        653  ACCGGC               [GA]^CCGG[CT]
DraII         212  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DraIII        109  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          223  GACCAGACGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          564  TGGCCG               [CT]^GGCC[GA]
EcoO109I      212  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         973  GAATTC               G^AATTC
EcoT14I       926  CCATGG               C^C[AT][AT]GG
KasI           60  GGCGCC               G^GCGCC
MaeI          376  CTAG                 C^TAG
MluI          534  ACGCGT               A^CGCGT
MseI         1253  TTAA                 T^TAA
NarI           60  GGCGCC               GG^CGCC
NcoI          926  CCATGG               C^CATGG
NspI          668  ACATGC               [GA]CATG^[CT]
PpuMI         212  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII        1237  CAGCTG               CAG^CTG
ScaI         1298  AGTACT               AGT^ACT
SfiI          556  GGCCTGGTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI           60  GGCGCC               GGC^GCC
SmaI          746  CCCGGG               CCC^GGG
StyI          926  CCATGG               C^C[AT][AT]GG
Tru9I        1253  TTAA                 T^TAA
XmaCI         746  CCCGGG               C^CCGGG
XmaI          746  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI           60  GGCGCC               G[GA]^CG[CT]C
               84  GGCGTC              
AlwI         1017  GATCC                GGATC(4/5)
             1393  GATCC               
Aor51HI        46  AGCGCT               AGC^GCT
             1467  AGCGCT              
BanI           60  GGCGCC               G^G[CT][GA]CC
              114  GGTGCC              
BbsI          162  GAAGAC               GAAGAC(2/6)
             1291  GAAGAC              
BpuAI         162  GAAGAC               GAAGAC(2/6)
             1291  GAAGAC              
BsaHI          60  GGCGCC               G[GA]^CG[CT]C
               84  GGCGTC              
BstEII        622  GGTGACC              G^GT[ACGT]ACC
             1311  GGTCACC             
BstPI         622  GGTGACC              G^GT[ACGT]ACC
             1311  GGTCACC             
BstYI        1016  AGATCC               [GA]^GATC[CT]
             1076  AGATCT              
Eco47III       46  AGCGCT               AGC^GCT
             1467  AGCGCT              
EcoO65I       622  GGTGACC              G^GT[ACGT]ACC
             1311  GGTCACC             
Hin1I          60  GGCGCC               G[GA]^CG[CT]C
               84  GGCGTC              
HincII        304  GTCAAC               GT[CT]^[GA]AC
             1164  GTTGAC              
HindII        304  GTCAAC               GT[CT]^[GA]AC
             1164  GTTGAC              
MaeII         278  ACGT                 A^CGT
             1346  ACGT                
MflI         1016  AGATCC               [GA]^GATC[CT]
             1076  AGATCT              
MspA1I        118  CCGCTG               C[AC]G^C[GT]G
             1237  CAGCTG              
PstI          540  CTGCAG               CTGCA^G
             1471  CTGCAG              
SapI          349  GAAGAGC              GCTCTTC(1/4)
              723  GCTCTTC             
SmlI          574  CTTGAG               C^T[CT][GA]AG
              898  CTTGAG              
StuI          555  AGGCCT               AGG^CCT
              799  AGGCCT              
TaqI          632  TCGA                 T^CGA
             1136  TCGA                
TfiI          424  GAATC                G^A[AT]TC
             1141  GAATC               
TthHB8I       632  TCGA                 T^CGA
             1136  TCGA                
XhoII        1016  AGATCC               [GA]^GATC[CT]
             1076  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
ApoI        AspHI       AvaII       BanII       BbvI        
BcnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaI        BsaJI       BsaWI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EarI        EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeIII      
MboI        MboII       MnlI        MslI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TseI        Tsp45I      Tsp509I     TspRI       
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