Restriction map for pF1KB0447
 (Linear) (Six-base) MAPSORT of: KIBB0447.seq  Check: 4737  from: 1  to: 1503



KIBB0447.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     460     678     765    1503 
   Size:        460     218      87     738

  Fragments arranged by size: 

                 738     460     218      87
 
AfeI AGC'GCT

Cuts at:      0      48    1469    1503 
   Size:         48    1421      34

  Fragments arranged by size: 

                1421      48      34
 
AflIII A'CryG_T

Cuts at:      0     534    1503 
   Size:        534     969
 
AleI CACnn'nnGTG

Cuts at:      0    1318    1366    1503 
   Size:       1318      48     137

  Fragments arranged by size: 

                1318     137      48
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     379     555     859    1503 
   Size:        379     176     304     644

  Fragments arranged by size: 

                 644     379     304     176
 
AlwNI CAG_nnn'CTG

Cuts at:      0     216    1503 
   Size:        216    1287
 
ApoI r'AATT_y

Cuts at:      0     290     973    1441    1503 
   Size:        290     683     468      62

  Fragments arranged by size: 

                 683     468     290      62
 
AvaI C'yCGr_G

Cuts at:      0     746    1503 
   Size:        746     757
 
BanI G'GyrC_C

Cuts at:      0      60     114    1503 
   Size:         60      54    1389

  Fragments arranged by size: 

                1389      60      54
 
BanII G_rGCy'C

Cuts at:      0     248     449     598     725     755     866    1136    1503 
   Size:        248     201     149     127      30     111     270     367

  Fragments arranged by size: 

                 367     270     248     201     149     127     111      30
            
 
BbeI G_GCGC'C

Cuts at:      0      64    1503 
   Size:         64    1439
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     169    1298    1503 
   Size:        169    1129     205

  Fragments arranged by size: 

                1129     205     169
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     753    1503 
   Size:        753     750
 
BglI GCCn_nnn'nGGC

Cuts at:      0     563    1503 
   Size:        563     940
 
BglII A'GATC_T

Cuts at:      0    1076    1503 
   Size:       1076     427
 
BlpI GC'TnA_GC

Cuts at:      0     367     917    1118    1503 
   Size:        367     550     201     385

  Fragments arranged by size: 

                 550     385     367     201
 
Bme1580I G_kGCm'C

Cuts at:      0      75     110    1503 
   Size:         75      35    1393

  Fragments arranged by size: 

                1393      75      35
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      62    1266    1503 
   Size:         62    1204     237

  Fragments arranged by size: 

                1204     237      62
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     126     232     664    1132    1252    1410    1503 
   Size:        126     106     432     468     120     158      93

  Fragments arranged by size: 

                 468     432     158     126     120     106      93
 
Bpu10I CC'TnA_GC

Cuts at:      0     552    1503 
   Size:        552     951
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     595     919    1503 
   Size:        595     324     584

  Fragments arranged by size: 

                 595     584     324
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     237     409     460    1444    1503 
   Size:        237     172      51     984      59

  Fragments arranged by size: 

                 984     237     172      59      51
 
BsaHI Gr'CG_yC

Cuts at:      0      61      85    1503 
   Size:         61      24    1418

  Fragments arranged by size: 

                1418      61      24
 
BsaWI w'CCGG_w

Cuts at:      0     794     998    1354    1503 
   Size:        794     204     356     149

  Fragments arranged by size: 

                 794     356     204     149
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      48      78    1066    1093    1096    1123    1207    1237 
   Size:         48      30     988      27       3      27      84      30

Cuts at:   1237    1503 
   Size:        266

  Fragments arranged by size: 

                 988     266      84      48      30      30      27      27
                   3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     110     592    1212    1503 
   Size:        110     482     620     291

  Fragments arranged by size: 

                 620     482     291     110
 
BsiEI CG_ry'CG

Cuts at:      0     654    1503 
   Size:        654     849
 
BsiHKAI G_wGCw'C

Cuts at:      0     248     449     598     725    1136    1503 
   Size:        248     201     149     127     411     367

  Fragments arranged by size: 

                 411     367     248     201     149     127
 
BsiWI C'GTAC_G

Cuts at:      0     857    1503 
   Size:        857     646
 
Bsp1286I G_dGCh'C

Cuts at:      0      75     110     248     449     598     725     755     866 
   Size:         75      35     138     201     149     127      30     111

Cuts at:    866    1136    1503 
   Size:        270     367

  Fragments arranged by size: 

                 367     270     201     149     138     127     111      75
                  35      30
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     201     523     782    1503 
   Size:        201     322     259     721

  Fragments arranged by size: 

                 721     322     259     201
 
BsrDI GCAATG_nn'

Cuts at:      0     468    1503 
   Size:        468    1035
 
BsrFI r'CCGG_y

Cuts at:      0     653    1503 
   Size:        653     850
 
BssHII G'CGCG_C

Cuts at:      0     100    1503 
   Size:        100    1403
 
BssSI C'ACGA_G

Cuts at:      0     910    1503 
   Size:        910     593
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     114    1503 
   Size:        114    1389
 
BstEII G'GTnAC_C

Cuts at:      0     622    1311    1503 
   Size:        622     689     192

  Fragments arranged by size: 

                 689     622     192
 
BstYI r'GATC_y

Cuts at:      0    1016    1076    1503 
   Size:       1016      60     427

  Fragments arranged by size: 

                1016     427      60
 
BtgI C'CryG_G

Cuts at:      0     926    1031    1316    1454    1503 
   Size:        926     105     285     138      49

  Fragments arranged by size: 

                 926     285     138     105      49
 
BtsI GCAGTG_nn'

Cuts at:      0     361    1503 
   Size:        361    1142
 
DraIII CAC_nnn'GTG

Cuts at:      0     114    1503 
   Size:        114    1389
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     229    1503 
   Size:        229    1274
 
EaeI y'GGCC_r

Cuts at:      0     564    1503 
   Size:        564     939
 
EarI CTCTTCn'nnn_

Cuts at:      0     344     482     730    1503 
   Size:        344     138     248     773

  Fragments arranged by size: 

                 773     344     248     138
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1009    1474    1503 
   Size:       1009     465      29

  Fragments arranged by size: 

                1009     465      29
 
EcoICRI GAG'CTC

Cuts at:      0     246     447     596     723    1134    1503 
   Size:        246     201     149     127     411     369

  Fragments arranged by size: 

                 411     369     246     201     149     127
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     126     232     460     664     678     765    1132    1252 
   Size:        126     106     228     204      14      87     367     120

Cuts at:   1252    1410    1503 
   Size:        158      93

  Fragments arranged by size: 

                 367     228     204     158     126     120     106      93
                  87      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0     213    1503 
   Size:        213    1290
 
EcoRI G'AATT_C

Cuts at:      0     973    1503 
   Size:        973     530
 
HaeII r_GCGC'y

Cuts at:      0      50      64    1471    1503 
   Size:         50      14    1407      32

  Fragments arranged by size: 

                1407      50      32      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     150     182     312     344    1066    1098    1503 
   Size:        150      32     130      32     722      32     405

  Fragments arranged by size: 

                 722     405     150     130      32      32      32
 
HincII GTy'rAC

Cuts at:      0     306    1166    1503 
   Size:        306     860     337

  Fragments arranged by size: 

                 860     337     306
 
KasI G'GCGC_C

Cuts at:      0      60    1503 
   Size:         60    1443
 
MluI A'CGCG_T

Cuts at:      0     534    1503 
   Size:        534     969
 
MslI CAynn'nnrTG

Cuts at:      0    1318    1366    1402    1503 
   Size:       1318      48      36     101

  Fragments arranged by size: 

                1318     101      48      36
 
MspA1I CmG'CkG

Cuts at:      0     120    1239    1503 
   Size:        120    1119     264

  Fragments arranged by size: 

                1119     264     120
 
NarI GG'CG_CC

Cuts at:      0      61    1503 
   Size:         61    1442
 
NcoI C'CATG_G

Cuts at:      0     926    1503 
   Size:        926     577
 
NspI r_CATG'y

Cuts at:      0     672    1503 
   Size:        672     831
 
PfoI T'CCnGG_A

Cuts at:      0     248     728    1426    1503 
   Size:        248     480     698      77

  Fragments arranged by size: 

                 698     480     248      77
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     580     612    1503 
   Size:        580      32     891

  Fragments arranged by size: 

                 891     580      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     213    1503 
   Size:        213    1290
 
PstI C_TGCA'G

Cuts at:      0     544    1475    1503 
   Size:        544     931      28

  Fragments arranged by size: 

                 931     544      28
 
PvuII CAG'CTG

Cuts at:      0    1239    1503 
   Size:       1239     264
 
SacI G_AGCT'C

Cuts at:      0     248     449     598     725    1136    1503 
   Size:        248     201     149     127     411     367

  Fragments arranged by size: 

                 411     367     248     201     149     127
 
SapI GCTCTTCn'nnn_

Cuts at:      0     344     730    1503 
   Size:        344     386     773

  Fragments arranged by size: 

                 773     386     344
 
ScaI AGT'ACT

Cuts at:      0    1300    1503 
   Size:       1300     203
 
SfcI C'TryA_G

Cuts at:      0     540    1415    1471    1503 
   Size:        540     875      56      32

  Fragments arranged by size: 

                 875     540      56      32
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     563    1503 
   Size:        563     940
 
SfoI GGC'GCC

Cuts at:      0      62    1503 
   Size:         62    1441
 
SmaI CCC'GGG

Cuts at:      0     748    1503 
   Size:        748     755
 
SmlI C'TyrA_G

Cuts at:      0     574     898    1503 
   Size:        574     324     605

  Fragments arranged by size: 

                 605     574     324
 
StuI AGG'CCT

Cuts at:      0     557     801    1503 
   Size:        557     244     702

  Fragments arranged by size: 

                 702     557     244
 
StyI C'CwwG_G

Cuts at:      0     926    1503 
   Size:        926     577
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     139    1248    1503 
   Size:        139    1109     255

  Fragments arranged by size: 

                1109     255     139
 
TatI w'GTAC_w

Cuts at:      0    1298    1503 
   Size:       1298     205
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     606     638    1503 
   Size:        606      32     865

  Fragments arranged by size: 

                 865     606      32
 
XmaI C'CCGG_G

Cuts at:      0     746    1503 
   Size:        746     757
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI     ApoI     AvaI
     BanI    BanII     BbeI     BbsI    BciVI     BglI    BglII     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspMI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstYI     BtgI     BtsI
   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    HaeII    Hin4I   HincII     KasI     MluI     MslI   MspA1I
     NarI     NcoI     NspI     PfoI     PpiI    PpuMI     PstI    PvuII
     SacI     SapI     ScaI     SfcI     SfiI     SfoI     SmaI     SmlI
     StuI     StyI    TaqII     TatI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI    BbvCI     BcgI     BclI    BfrBI    BmgBI     BmtI     BplI
    BsaAI    BsaBI    BseYI     BsgI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrGI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI     EagI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KpnI     MfeI     MmeI     MscI     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI    SexAI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com