Restriction map for pF1KB6523
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        AlwNI       AocI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiWI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraII       DrdI        
DsaI        Eam1105I    Eco47III    Eco81I      EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FseI        HaeII       HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tth111I     TthHB8I     XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI         1039  GTCTAC               GT^[AC][GT]AC
AcsI          271  AAATTC               [GA]^AATT[CT]
AfaI          483  GTAC                 GT^AC
AflIII        924  ACGTGT               A^C[GA][CT]GT
Alw44I        928  GTGCAC               G^TGCAC
ApaI          172  GGGCCC               GGGCC^C
ApaLI         928  GTGCAC               G^TGCAC
ApoI          271  AAATTC               [GA]^AATT[CT]
AspHI         928  GTGCAC               G[AT]GC[AT]^C
AviII         542  TGCGCA               TGC^GCA
BbsI          702  GAAGAC               GAAGAC(2/6)
BclI          554  TGATCA               T^GATCA
BfaI         1081  CTAG                 C^TAG
BglII         498  AGATCT               A^GATCT
BpuAI         702  GAAGAC               GAAGAC(2/6)
BsgI         1003  GTGCAG               GTGCAG(16/14)
BsiEI        1047  CGGCCG               CG[GA][CT]^CG
BsiHKAI       928  GTGCAC               G[AT]GC[AT]^C
BsmBI        1013  GAGACG               CGTCTC(1/5)
BsrDI         518  GCAATG               GCAATG(2/0)
BsrFI        1045  ACCGGC               [GA]^CCGG[CT]
BsrI          547  ACTGG                ACTGG(1/-1)
BstXI         138  CCAGGCCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I        1021  GCGGGC               GC[ACGT]^[ACGT]GC
CfoI          543  GCGC                 GCG^C
Cfr10I       1045  ACCGGC               [GA]^CCGG[CT]
DraIII        931  CACCGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1047  CGGCCG               C^GGCCG
EclXI        1047  CGGCCG               C^GGCCG
Eco52I       1047  CGGCCG               C^GGCCG
EcoNI         780  CCTGGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          554  TGATCA               T^GATCA
FspI          542  TGCGCA               TGC^GCA
HhaI          543  GCGC                 GCG^C
HinP1I        543  GCGC                 G^CGC
HincII        253  GTCAAC               GT[CT]^[GA]AC
HindII        253  GTCAAC               GT[CT]^[GA]AC
HphI          552  GGTGA                GGTGA(8/7)
MaeI         1081  CTAG                 C^TAG
MslI          546  CACTGGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         70  CAGCGG               C[AC]G^C[GT]G
PflMI         993  CCAGATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI          483  GTAC                 GT^AC
SexAI         435  ACCTGGT              A^CC[AT]GGT
SfaNI         416  GATGC                GCATC(5/9)
SmlI          982  CTCAAG               C^T[CT][GA]AG
Tsp45I        937  GTGAC                ^GT[GC]AC
Van91I        993  CCAGATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1080  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AccII         934  CGCG                 CG^CG
              961  CGCG                
AluI          162  AGCT                 AG^CT
              248  AGCT                
AvaII         909  GGTCC                G^G[AT]CC
              990  GGACC               
BanII         133  GAGCCC               G[GA]GC[CT]^C
              172  GGGCCC              
BcnI          320  CCCGG                CC^[GC]GG
              912  CCGGG               
BstUI         934  CGCG                 CG^CG
              961  CGCG                
EaeI          638  CGGCCA               [CT]^GGCC[GA]
             1047  CGGCCG              
MaeII         924  ACGT                 A^CGT
             1031  ACGT                
MaeIII         84  GTTAC                ^GT[ACGT]AC
              937  GTGAC               
MvnI          934  CGCG                 CG^CG
              961  CGCG                
NciI          320  CCCGG                CC^[GC]GG
              912  CCGGG               
Tsp509I       272  AATT                 ^AATT
             1089  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AlwI        BbvI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BssKI       BstF5I      BstNI       
BstYI       Cfr13I      DdeI        DpnII       EarI        
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       ItaI        Ksp632I     
MboI        MboII       MflI        MnlI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       SfcI        
TseI        TspRI       XhoII       
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