Restriction map for pF1KB0421
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AcyI        
AflII       AflIII      AgeI        Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AviII       AvrII       BanI        
BbeI        BbrPI       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsgI        BsiEI       
BsiWI       BsmI        BspDI       BspHI       BspLU11I    
BspMI       BsrDI       Bst1107I    BstAPI      BstBI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DrdI        DsaI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MseI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspI        
StuI        SwaI        Tru9I       XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          976  GTAGAC               GT^[AC][GT]AC
AccIII        244  TCCGGA               T^CCGGA
AhdI          581  GACCCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         1139  GGGCCC               GGGCC^C
AspEI         581  GACCCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         796  GAGCAC               G[AT]GC[AT]^C
BamHI         137  GGATCC               G^GATCC
BbvCI        1102  CCTCAGC              CCTCAGC(-5/-2)
BpmI          779  CTCCAG               CTGGAG(16/14)
BsaWI         244  TCCGGA               [AT]^CCGG[AT]
BseAI         244  TCCGGA               T^CCGGA
BsiHKAI       796  GAGCAC               G[AT]GC[AT]^C
BsmBI        1209  GAGACG               CGTCTC(1/5)
Bsp1407I      729  TGTACA               T^GTACA
BspEI         244  TCCGGA               T^CCGGA
BsrBI          28  GAGCGG               CCGCTC(-3/-3)
BsrFI          47  ACCGGC               [GA]^CCGG[CT]
BsrGI         729  TGTACA               T^GTACA
BssHII        161  GCGCGC               G^CGCGC
BstEII       1095  GGTGACC              G^GT[ACGT]ACC
BstF5I        945  CATCC                GGATG(2/0)
BstPI        1095  GGTGACC              G^GT[ACGT]ACC
BstXI         746  CCAAGGAATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         137  GGATCC               [GA]^GATC[CT]
Cfr10I         47  ACCGGC               [GA]^CCGG[CT]
DraII         269  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DraIII        722  CACTCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      581  GACCCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          484  GAAGAG               CTCTTC(1/4)
EcoO109I      269  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1095  GGTGACC              G^GT[ACGT]ACC
FauI          402  GCGGG                CCCGC(4/6)
FokI          945  CATCC                GGATG(9/13)
HgaI          176  GCGTC                GACGC(5/10)
Ksp632I       484  GAAGAG               CTCTTC(1/4)
MaeII         143  ACGT                 A^CGT
MflI          137  GGATCC               [GA]^GATC[CT]
MroI          244  TCCGGA               T^CCGGA
MslI          387  CATGGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI         543  CCAAGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         269  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI         1156  CTGCAG               CTGCA^G
SbfI         1155  CCTGCAGG             CCTGCA^GG
SfaNI         607  GATGC                GCATC(5/9)
Sse8387I     1155  CCTGCAGG             CCTGCA^GG
SspBI         729  TGTACA               T^GTACA
TaqI          299  TCGA                 T^CGA
TspRI         261  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       299  TCGA                 T^CGA
Van91I        543  CCAAGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         137  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII          93  CGCG                 CG^CG
              162  CGCG                
AfaI          553  GTAC                 GT^AC
              730  GTAC                
AlwNI         522  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1061  CAGCCTCTG           
AocI           11  CCTCAGG              CC^T[ACGT]AGG
              478  CCTGAGG             
AspI          172  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              597  GACGGTGTC           
AvaI          133  CTCGGG               C^[CT]CG[GA]G
              377  CTCGGG              
BfaI          217  CTAG                 C^TAG
              697  CTAG                
BglI           24  GCCGGAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1216  GCCCAGGAGGC         
BsoBI         133  CTCGGG               C^[CT]CG[GA]G
              377  CTCGGG              
BstUI          93  CGCG                 CG^CG
              162  CGCG                
Bsu36I         11  CCTCAGG              CC^T[ACGT]AGG
              478  CCTGAGG             
DpnII         138  GATC                 ^GATC
              256  GATC                
Eco81I         11  CCTCAGG              CC^T[ACGT]AGG
              478  CCTGAGG             
EcoT14I       746  CCAAGG               C^C[AT][AT]GG
             1037  CCAAGG              
MaeI          217  CTAG                 C^TAG
              697  CTAG                
MboI          138  GATC                 ^GATC
              256  GATC                
MvnI           93  CGCG                 CG^CG
              162  CGCG                
NdeII         138  GATC                 ^GATC
              256  GATC                
PflFI         172  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              597  GACGGTGTC           
RsaI          553  GTAC                 GT^AC
              730  GTAC                
Sau3AI        138  GATC                 ^GATC
              256  GATC                
SfcI          330  CTGTAG               C^T[GA][CT]AG
             1156  CTGCAG              
SmlI          424  CTCAAG               C^T[CT][GA]AG
             1000  CTTGAG              
StyI          746  CCAAGG               C^C[AT][AT]GG
             1037  CCAAGG              
Tsp509I       316  AATT                 ^AATT
              752  AATT                
Tth111I       172  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              597  GACGGTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BalI        
BanII       BbsI        BbvI        BcnI        BmyI        
BpuAI       BsaI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BssSI       BstNI       Cac8I       CfoI        
Cfr13I      DdeI        EaeI        EcoRII      Fnu4HI      
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboII       
MluNI       MnlI        MscI        MspA1I      MspI        
MvaI        MwoI        NciI        NlaIII      NlaIV       
PleI        PvuII       Sau96I      ScrFI       TfiI        
TseI        Tsp45I      
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