Restriction map for pF1KB0415
 (Linear) (Six-base) MAPSORT of: KIBB0415.seq  Check: 5134  from: 1  to: 1404



KIBB0415.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1017    1064    1210    1404 
   Size:       1017      47     146     194

  Fragments arranged by size: 

                1017     194     146      47
 
AgeI A'CCGG_T

Cuts at:      0    1290    1404 
   Size:       1290     114
 
AleI CACnn'nnGTG

Cuts at:      0     676    1264    1404 
   Size:        676     588     140

  Fragments arranged by size: 

                 676     588     140
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     893    1404 
   Size:        893     511
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     964     996    1404 
   Size:        964      32     408

  Fragments arranged by size: 

                 964     408      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     673    1404 
   Size:        673     731
 
ApaLI G'TGCA_C

Cuts at:      0     316    1404 
   Size:        316    1088
 
AvaI C'yCGr_G

Cuts at:      0     141     340    1326    1404 
   Size:        141     199     986      78

  Fragments arranged by size: 

                 986     199     141      78
 
BanII G_rGCy'C

Cuts at:      0     350     376     764     922    1188    1366    1404 
   Size:        350      26     388     158     266     178      38

  Fragments arranged by size: 

                 388     350     266     178     158      38      26
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     164     482    1174    1404 
   Size:        164     318     692     230

  Fragments arranged by size: 

                 692     318     230     164
 
BbvCI CC'TCA_GC

Cuts at:      0     247     379    1404 
   Size:        247     132    1025

  Fragments arranged by size: 

                1025     247     132
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      81     115    1404 
   Size:         81      34    1289

  Fragments arranged by size: 

                1289      81      34
 
BfrBI ATG'CAT

Cuts at:      0    1212    1404 
   Size:       1212     192
 
BglI GCCn_nnn'nGGC

Cuts at:      0      37    1404 
   Size:         37    1367
 
Bme1580I G_kGCm'C

Cuts at:      0     320     882     937    1404 
   Size:        320     562      55     467

  Fragments arranged by size: 

                 562     467     320      55
 
BmgBI CAC'GTC

Cuts at:      0    1172    1404 
   Size:       1172     232
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     186    1057    1258    1404 
   Size:        186     871     201     146

  Fragments arranged by size: 

                 871     201     186     146
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     802     867     947    1130    1404 
   Size:        802      65      80     183     274

  Fragments arranged by size: 

                 802     274     183      80      65
 
Bpu10I CC'TnA_GC

Cuts at:      0     184     247     379    1404 
   Size:        184      63     132    1025

  Fragments arranged by size: 

                1025     184     132      63
 
BsaAI yAC'GTr

Cuts at:      0     864    1404 
   Size:        864     540
 
BsaHI Gr'CG_yC

Cuts at:      0     842    1404 
   Size:        842     562
 
BsaWI w'CCGG_w

Cuts at:      0     365    1290    1404 
   Size:        365     925     114

  Fragments arranged by size: 

                 925     365     114
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     110     140     709     739    1404 
   Size:        110      30     569      30     665

  Fragments arranged by size: 

                 665     569     110      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     358     901    1126    1404 
   Size:        358     543     225     278

  Fragments arranged by size: 

                 543     358     278     225
 
BseYI C'CCAG_C

Cuts at:      0     211     303     307     350    1300    1404 
   Size:        211      92       4      43     950     104

  Fragments arranged by size: 

                 950     211     104      92      43       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     889    1404 
   Size:        277     612     515

  Fragments arranged by size: 

                 612     515     277
 
BsiEI CG_ry'CG

Cuts at:      0     300     473    1404 
   Size:        300     173     931

  Fragments arranged by size: 

                 931     300     173
 
BsiHKAI G_wGCw'C

Cuts at:      0     185     320     415     443     764    1404 
   Size:        185     135      95      28     321     640

  Fragments arranged by size: 

                 640     321     185     135      95      28
 
Bsp1286I G_dGCh'C

Cuts at:      0     185     320     350     376     415     443     764     882 
   Size:        185     135      30      26      39      28     321     118

Cuts at:    882     922     937    1188    1366    1404 
   Size:         40      15     251     178      38

  Fragments arranged by size: 

                 321     251     185     178     135     118      40      39
                  38      30      28      26      15
 
BspEI T'CCGG_A

Cuts at:      0     365    1404 
   Size:        365    1039
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     145    1404 
   Size:        145    1259
 
BsrBI CCG'CTC

Cuts at:      0      52     851    1404 
   Size:         52     799     553

  Fragments arranged by size: 

                 799     553      52
 
BsrDI GCAATG_nn'

Cuts at:      0     390    1142    1404 
   Size:        390     752     262

  Fragments arranged by size: 

                 752     390     262
 
BsrFI r'CCGG_y

Cuts at:      0     192     295    1290    1404 
   Size:        192     103     995     114

  Fragments arranged by size: 

                 995     192     114     103
 
BssHII G'CGCG_C

Cuts at:      0      17    1404 
   Size:         17    1387
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     867    1404 
   Size:        867     537
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     271    1101    1308    1404 
   Size:        271     830     207      96

  Fragments arranged by size: 

                 830     271     207      96
 
Bsu36I CC'TnA_GG

Cuts at:      0     856    1404 
   Size:        856     548
 
BtgI C'CryG_G

Cuts at:      0     677     929    1357    1404 
   Size:        677     252     428      47

  Fragments arranged by size: 

                 677     428     252      47
 
BtsI GCAGTG_nn'

Cuts at:      0     671    1087    1404 
   Size:        671     416     317

  Fragments arranged by size: 

                 671     416     317
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     886     921    1404 
   Size:        886      35     483

  Fragments arranged by size: 

                 886     483      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     180     867    1404 
   Size:        180     687     537

  Fragments arranged by size: 

                 687     537     180
 
EaeI y'GGCC_r

Cuts at:      0       5      38     297     470     680    1404 
   Size:          5      33     259     173     210     724

  Fragments arranged by size: 

                 724     259     210     173      33       5
 
EagI C'GGCC_G

Cuts at:      0     297     470    1404 
   Size:        297     173     934

  Fragments arranged by size: 

                 934     297     173
 
EarI CTCTTCn'nnn_

Cuts at:      0     364    1036    1404 
   Size:        364     672     368

  Fragments arranged by size: 

                 672     368     364
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     641    1205    1404 
   Size:        641     564     199

  Fragments arranged by size: 

                 641     564     199
 
EcoICRI GAG'CTC

Cuts at:      0     762    1404 
   Size:        762     642
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     802     867     947    1017    1064    1130    1210    1404 
   Size:        802      65      80      70      47      66      80     194

  Fragments arranged by size: 

                 802     194      80      80      70      66      65      47
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     403     813    1353    1404 
   Size:        403     410     540      51

  Fragments arranged by size: 

                 540     410     403      51
 
EcoRV GAT'ATC

Cuts at:      0     769    1404 
   Size:        769     635
 
FseI GG_CCGG'CC

Cuts at:      0     299    1404 
   Size:        299    1105
 
FspI TGC'GCA

Cuts at:      0      80    1404 
   Size:         80    1324
 
HaeII r_GCGC'y

Cuts at:      0      58      72    1404 
   Size:         58      14    1332

  Fragments arranged by size: 

                1332      58      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     709     741    1309    1341    1404 
   Size:        709      32     568      32      63

  Fragments arranged by size: 

                 709     568      63      32      32
 
MscI TGG'CCA

Cuts at:      0     682    1404 
   Size:        682     722
 
MslI CAynn'nnrTG

Cuts at:      0     676     827     988    1264    1404 
   Size:        676     151     161     276     140

  Fragments arranged by size: 

                 676     276     161     151     140
 
MspA1I CmG'CkG

Cuts at:      0     307     695     967    1236    1404 
   Size:        307     388     272     269     168

  Fragments arranged by size: 

                 388     307     272     269     168
 
NaeI GCC'GGC

Cuts at:      0     194     297    1404 
   Size:        194     103    1107

  Fragments arranged by size: 

                1107     194     103
 
NcoI C'CATG_G

Cuts at:      0     929    1404 
   Size:        929     475
 
NgoMIV G'CCGG_C

Cuts at:      0     192     295    1404 
   Size:        192     103    1109

  Fragments arranged by size: 

                1109     192     103
 
NsiI A_TGCA'T

Cuts at:      0    1214    1404 
   Size:       1214     190
 
NspI r_CATG'y

Cuts at:      0    1212    1404 
   Size:       1212     192
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     218    1404 
   Size:        218    1186
 
PfoI T'CCnGG_A

Cuts at:      0     482     539     727     952    1241    1404 
   Size:        482      57     188     225     289     163

  Fragments arranged by size: 

                 482     289     225     188     163      57
 
PmlI CAC'GTG

Cuts at:      0     864    1404 
   Size:        864     540
 
PpuMI rG'GwC_Cy

Cuts at:      0     403     813    1353    1404 
   Size:        403     410     540      51

  Fragments arranged by size: 

                 540     410     403      51
 
PspXI vC'TCGA_Gb

Cuts at:      0    1326    1404 
   Size:       1326      78
 
PstI C_TGCA'G

Cuts at:      0     201    1404 
   Size:        201    1203
 
PvuII CAG'CTG

Cuts at:      0     307     967    1236    1404 
   Size:        307     660     269     168

  Fragments arranged by size: 

                 660     307     269     168
 
SacI G_AGCT'C

Cuts at:      0     764    1404 
   Size:        764     640
 
SanDI GG'GwC_CC

Cuts at:      0    1353    1404 
   Size:       1353      51
 
SapI GCTCTTCn'nnn_

Cuts at:      0     364    1404 
   Size:        364    1040
 
SfcI C'TryA_G

Cuts at:      0     197     744    1404 
   Size:        197     547     660

  Fragments arranged by size: 

                 660     547     197
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1290    1404 
   Size:       1290     114
 
SmaI CCC'GGG

Cuts at:      0     143    1404 
   Size:        143    1261
 
SmlI C'TyrA_G

Cuts at:      0    1326    1404 
   Size:       1326      78
 
SphI G_CATG'C

Cuts at:      0    1212    1404 
   Size:       1212     192
 
SspI AAT'ATT

Cuts at:      0    1026    1404 
   Size:       1026     378
 
StuI AGG'CCT

Cuts at:      0    1251    1404 
   Size:       1251     153
 
StyI C'CwwG_G

Cuts at:      0      64     929     940    1404 
   Size:         64     865      11     464

  Fragments arranged by size: 

                 865     464      64      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     168    1404 
   Size:        168    1236
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     110     142    1404 
   Size:        110      32    1262

  Fragments arranged by size: 

                1262     110      32
 
XhoI C'TCGA_G

Cuts at:      0    1326    1404 
   Size:       1326      78
 
XmaI C'CCGG_G

Cuts at:      0     141    1404 
   Size:        141    1263
 
XmnI GAAnn'nnTTC

Cuts at:      0     279    1404 
   Size:        279    1125
 

 Enzymes that do cut:

     AcuI     AgeI     AleI     AlfI     AloI    AlwNI    ApaLI     AvaI
    BanII     BbsI    BbvCI     BcgI    BfrBI     BglI Bme1580I    BmgBI
     BmrI     BpmI   Bpu10I    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII   BstAPI    BstXI   Bsu36I     BtgI     BtsI
    CspCI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRV     FseI     FspI    HaeII    Hin4I     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NsiI     NspI    PflMI     PfoI
     PmlI    PpuMI    PspXI     PstI    PvuII     SacI    SanDI     SapI
     SfcI    SgrAI     SmaI     SmlI     SphI     SspI     StuI     StyI
    TaqII     TstI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     ApaI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BciVI     BclI    BglII     BlpI     BmtI
     BplI    BpuEI     BsaI    BsaBI    BsiWI     BsmI    BsmBI    BspHI
    BsrGI    BssSI    BstBI   BstEII    BstYI  BstZ17I    BtgZI     ClaI
     DraI     DrdI    EcoNI    EcoRI     FalI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NarI     NdeI
     NheI     NotI     NruI     PacI     PasI     PciI     PmeI     PpiI
    PshAI     PsiI   PspOMI     PsrI     PvuI    RsrII    SacII     SalI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SnaBI     SpeI
     SrfI     SwaI     TatI  Tth111I     XbaI     XcmI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com