################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII AccII AccIII AclI AflII AflIII AgeI ApaI AscI Asp700I AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BstAPI BstBI BstEII BstPI BstUI BstXI BstYI Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HpaI KspI MamI MfeI MflI MluI MroI MspA1I MunI MvnI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 70 enzymes) # ################################### Acc65I 207 GGTACC G^GTACC AccI 454 GTATAC GT^[AC][GT]AC AcyI 439 GGCGCC G[GA]^CG[CT]C AhdI 141 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1281 GTGCAC G^TGCAC AlwNI 1195 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 850 CCTAAGG CC^T[ACGT]AGG Aor51HI 986 AGCGCT AGC^GCT ApaLI 1281 GTGCAC G^TGCAC AseI 48 ATTAAT AT^TAAT AsnI 48 ATTAAT AT^TAAT Asp718I 207 GGTACC G^GTACC AspEI 141 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 434 CTCGGG C^[CT]CG[GA]G BalI 198 TGGCCA TGG^CCA BanII 882 GAGCCC G[GA]GC[CT]^C BbeI 439 GGCGCC GGCGC^C BbsI 1051 GAAGAC GAAGAC(2/6) BfaI 767 CTAG C^TAG BlpI 998 GCTCAGC GC^T[ACGT]AGC Bpu1102I 998 GCTCAGC GC^T[ACGT]AGC BpuAI 1051 GAAGAC GAAGAC(2/6) BsaHI 439 GGCGCC G[GA]^CG[CT]C BsgI 900 GTGCAG GTGCAG(16/14) BsmAI 183 GTCTC GTCTC(1/5) BsoBI 434 CTCGGG C^[CT]CG[GA]G Bsp1407I 1019 TGTACA T^GTACA BspHI 753 TCATGA T^CATGA BsrGI 1019 TGTACA T^GTACA BssSI 559 CACGAG CACGAG(-5/-1) Bst1107I 454 GTATAC GTA^TAC BstZ17I 454 GTATAC GTA^TAC Bsu36I 850 CCTAAGG CC^T[ACGT]AGG CelII 998 GCTCAGC GC^T[ACGT]AGC DraI 60 TTTAAA TTT^AAA DsaI 211 CCATGG C^C[GA][CT]GG EaeI 198 TGGCCA [CT]^GGCC[GA] Eam1105I 141 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 986 AGCGCT AGC^GCT Eco81I 850 CCTAAGG CC^T[ACGT]AGG EcoT14I 211 CCATGG C^C[AT][AT]GG FauI 1167 CCCGC CCCGC(4/6) HapII 555 CCGG C^CGG Hin1I 439 GGCGCC G[GA]^CG[CT]C HindIII 1097 AAGCTT A^AGCTT HpaII 555 CCGG C^CGG KasI 439 GGCGCC G^GCGCC KpnI 207 GGTACC GGTAC^C MaeI 767 CTAG C^TAG MaeII 357 ACGT A^CGT MluNI 198 TGGCCA TGG^CCA MscI 198 TGGCCA TGG^CCA MspI 555 CCGG C^CGG NarI 439 GGCGCC GG^CGCC NcoI 211 CCATGG C^CATGG PacI 49 TTAATTAA TTAAT^TAA PpuMI 854 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1066 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 48 ATTAAT AT^TAAT PstI 1068 CTGCAG CTGCA^G RcaI 753 TCATGA T^CATGA SapI 109 GAAGAGC GCTCTTC(1/4) SfaNI 1308 GATGC GCATC(5/9) SfoI 439 GGCGCC GGC^GCC SspBI 1019 TGTACA T^GTACA SspI 412 AATATT AAT^ATT StyI 211 CCATGG C^C[AT][AT]GG SwaI 59 ATTTAAAT ATTT^AAAT TaqI 1337 TCGA T^CGA TthHB8I 1337 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcsI 320 GAATTT [GA]^AATT[CT] 679 GAATTT ApoI 320 GAATTT [GA]^AATT[CT] 679 GAATTT AspHI 12 GAGCAC G[AT]GC[AT]^C 1281 GTGCAC BbvI 189 GCTGC GCAGC(8/12) 1230 GCTGC BsiHKAI 12 GAGCAC G[AT]GC[AT]^C 1281 GTGCAC BsmFI 717 GGGAC GGGAC(10/14) 1377 GGGAC BsrDI 1056 CATTGC GCAATG(2/0) 1266 CATTGC DraII 854 AGGACCT [GA]G^G[ACGT]CC[CT] 1157 AGGCCCT EarI 34 GAAGAG CTCTTC(1/4) 109 GAAGAG EcoO109I 854 AGGACCT [GA]G^G[ACGT]CC[CT] 1157 AGGCCCT Ksp632I 34 GAAGAG CTCTTC(1/4) 109 GAAGAG NspI 705 GCATGT [GA]CATG^[CT] 956 ACATGC PleI 1204 GACTC GAGTC(4/5) 1197 GAGTC SfcI 930 CTACAG C^T[GA][CT]AG 1068 CTGCAG SmlI 712 CTCAAG C^T[CT][GA]AG 1312 CTCAAG TseI 189 GCTGC G^C[AT]GC 1230 GCTGC Tsp45I 23 GTGAC ^GT[GC]AC 697 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BanI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeII HaeIII HhaI HinP1I HinfI HphI ItaI MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TfiI Tru9I Tsp509I TspRI