Restriction map for pF1KB6456
 (Circular) (Six-base) MAPSORT of: pF1KB6456.seq  Check: 5434  from: 1  to: 4166



pF1KB6456  4166 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AccI GT'mk_AC

Cuts at:    314    1204     314 
   Size:        890    3276
 
Acc65I G'GTAC_C

Cuts at:   1182    1182 
   Size:       4166
 
AclI AA'CG_TT

Cuts at:   4006    4006 
   Size:       4166
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    149     830    1991    2423    3170    3438     149 
   Size:        681    1161     432     747     268     877

  Fragments arranged by size: 

                1161     877     747     681     432     268
 
AfeI AGC'GCT

Cuts at:    436     436 
   Size:       4166
 
AflIII A'CryG_T

Cuts at:   1518    2622    3599    1518 
   Size:       1104     977    2085

  Fragments arranged by size: 

                2085    1104     977
 
AgeI A'CCGG_T

Cuts at:   2554    2554 
   Size:       4166
 
AlwNI CAG_nnn'CTG

Cuts at:   1471    3038    1471 
   Size:       1567    2599
 
ApaLI G'TGCA_C

Cuts at:   2936    2936 
   Size:       4166
 
ApoI r'AATT_y

Cuts at:    423     488     565    1071    1170    3519    3775     423 
   Size:         65      77     506      99    2349     256     814

  Fragments arranged by size: 

                2349     814     506     256      99      77      65
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4166
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4166
 
AvaI C'yCGr_G

Cuts at:   1186    1186 
   Size:       4166
 
BamHI G'GATC_C

Cuts at:   1191    1191 
   Size:       4166
 
BanI G'GyrC_C

Cuts at:   1182    1596    1844    1879    1182 
   Size:        414     248      35    3469

  Fragments arranged by size: 

                3469     414     248      35
 
BanII G_rGCy'C

Cuts at:   1180    2210    1180 
   Size:       1030    3136
 
BbeI G_GCGC'C

Cuts at:   1600    1848    1600 
   Size:        248    3918
 
BbsI GAAGACnn'nnnn_

Cuts at:   1131    1131 
   Size:       4166
 
BciVI GTATCCnnnnn_n'

Cuts at:   2064    2825    4166    2064 
   Size:        761    1341    2064

  Fragments arranged by size: 

                2064    1341     761
 
BclI T'GATC_A

Cuts at:   1686    1686 
   Size:       4166
 
BglII A'GATC_T

Cuts at:   1681    3718    1681 
   Size:       2037    2129
 
BlpI GC'TnA_GC

Cuts at:   1278    1278 
   Size:       4166
 
Bme1580I G_kGCm'C

Cuts at:   1791    1884    2940    1791 
   Size:         93    1056    3017

  Fragments arranged by size: 

                3017    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1378    1459    1794    3938    1378 
   Size:         81     335    2144    1606

  Fragments arranged by size: 

                2144    1606     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2533    2533 
   Size:       4166
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1342    2221    2713    3011    1342 
   Size:        879     492     298    2497

  Fragments arranged by size: 

                2497     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3870    3870 
   Size:       4166
 
BsaAI yAC'GTr

Cuts at:   1519    1519 
   Size:       4166
 
BsaBI GATnn'nnATC

Cuts at:      6    1680    3717       6 
   Size:       1674    2037     455

  Fragments arranged by size: 

                2037    1674     455
 
BsaHI Gr'CG_yC

Cuts at:   1597    1845    2547    4043    1597 
   Size:        248     702    1496    1720

  Fragments arranged by size: 

                1720    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1355    1876    2554    2828    2975    3386    1355 
   Size:        521     678     274     147     411    2135

  Fragments arranged by size: 

                2135     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1591    2349    2926    3422    1591 
   Size:        758     577     496    2335

  Fragments arranged by size: 

                2335     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    472     472 
   Size:       4166
 
BsiEI CG_ry'CG

Cuts at:    104     137    1708    1754    2962     104 
   Size:         33    1571      46    1208    1308

  Fragments arranged by size: 

                1571    1308    1208      46      33
 
BsiHKAI G_wGCw'C

Cuts at:   1180    1958    2148    2940    1180 
   Size:        778     190     792    2406

  Fragments arranged by size: 

                2406     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:   1180    1791    1884    1958    2148    2210    2940    1180 
   Size:        611      93      74     190      62     730    2406

  Fragments arranged by size: 

                2406     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3386    3386 
   Size:       4166
 
BspHI T'CATG_A

Cuts at:   3342    3342 
   Size:       4166
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    444    1216    1430    1732    2113     444 
   Size:        772     214     302     381    2497

  Fragments arranged by size: 

                2497     772     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2458    2512    3996    4161      50 
   Size:       2408      54    1484     165      55

  Fragments arranged by size: 

                2408    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2078    2078 
   Size:       4166
 
BsrFI r'CCGG_y

Cuts at:    227    2164    2345    2554     227 
   Size:       1937     181     209    1839

  Fragments arranged by size: 

                1937    1839     209     181
 
BsrGI T'GTAC_A

Cuts at:    518     518 
   Size:       4166
 
BssSI C'ACGA_G

Cuts at:   2437    2795    2437 
   Size:        358    3808
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1543    1543 
   Size:       4166
 
BstBI TT'CG_AA

Cuts at:   2527    2527 
   Size:       4166
 
BstYI r'GATC_y

Cuts at:      1     327     549     706    1191    1658    1681    2016    2262 
   Size:        326     222     157     485     467      23     335     246

Cuts at:   2262    3274    3360    3372    3718       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     485     467     449     346     335     326     246
                 222     157      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4166
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    642    2116    2297    2460     642 
   Size:       1474     181     163    2348

  Fragments arranged by size: 

                2348    1474     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1409    1409 
   Size:       4166
 
DraI TTT'AAA

Cuts at:   1165    1165 
   Size:       4166
 
DrdI GACnn_nn'nnGTC

Cuts at:   1872    2730    1872 
   Size:        858    3308
 
EaeI y'GGCC_r

Cuts at:    134    1751    1925    2316    2343    3688     134 
   Size:       1617     174     391      27    1345     612

  Fragments arranged by size: 

                1617    1345     612     391     174      27
 
EagI C'GGCC_G

Cuts at:    134    1751     134 
   Size:       1617    2549
 
EarI CTCTTCn'nnn_

Cuts at:    194     302     730    2189    2399     194 
   Size:        108     428    1459     210    1961

  Fragments arranged by size: 

                1961    1459     428     210     108
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2374    2684    2830    3976    2374 
   Size:        310     146    1146    2564

  Fragments arranged by size: 

                2564    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1178    1178 
   Size:       4166
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    149     830    1991    2423    3170    3438     149 
   Size:        681    1161     432     747     268     877

  Fragments arranged by size: 

                1161     877     747     681     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:   1305    1305 
   Size:       4166
 
EcoRI G'AATT_C

Cuts at:   1170    1170 
   Size:       4166
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2185    2185 
   Size:       4166
 
FspI TGC'GCA

Cuts at:   1947    1947 
   Size:       4166
 
HaeII r_GCGC'y

Cuts at:    438    1600    1848    2870    3849     438 
   Size:       1162     248    1022     979     755

  Fragments arranged by size: 

                1162    1022     979     755     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    142     145     174     177     631     663    2008    2038    2040 
   Size:          3      29       3     454      32    1345      30       2

Cuts at:   2040    2070     142 
   Size:         30    2238

  Fragments arranged by size: 

                2238    1345     454      32      30      30      29       3
                   3       2
 
HincII GTy'rAC

Cuts at:    315    1205     315 
   Size:        890    3276
 
HindIII A'AGCT_T

Cuts at:    346     346 
   Size:       4166
 
KasI G'GCGC_C

Cuts at:   1596    1844    1596 
   Size:        248    3918
 
KpnI G_GTAC'C

Cuts at:   1186    1186 
   Size:       4166
 
MfeI C'AATT_G

Cuts at:    581     581 
   Size:       4166
 
MluI A'CGCG_T

Cuts at:   3599    3599 
   Size:       4166
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2837    3021    2837 
   Size:        184    3982
 
MscI TGG'CCA

Cuts at:   1927    1927 
   Size:       4166
 
MslI CAynn'nnrTG

Cuts at:    934     949    2155    2282     934 
   Size:         15    1206     127    2818

  Fragments arranged by size: 

                2818    1206     127      15
 
MspA1I CmG'CkG

Cuts at:   1277    1591    1951    2964    3209    1277 
   Size:        314     360    1013     245    2234

  Fragments arranged by size: 

                2234    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2347    2347 
   Size:       4166
 
NarI GG'CG_CC

Cuts at:   1597    1845    1597 
   Size:        248    3918
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4166
 
NgoMIV G'CCGG_C

Cuts at:   2345    2345 
   Size:       4166
 
NspI r_CATG'y

Cuts at:   1219    1719    2626    1219 
   Size:        500     907    2759

  Fragments arranged by size: 

                2759     907     500
 
PciI A'CATG_T

Cuts at:   2622    2622 
   Size:       4166
 
PmeI GTTT'AAAC

Cuts at:   1165    1165 
   Size:       4166
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3305    3337    3305 
   Size:         32    4134
 
PstI C_TGCA'G

Cuts at:   1213    1898    1213 
   Size:        685    3481
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4166
 
PvuII CAG'CTG

Cuts at:   1591    1951    1591 
   Size:        360    3806
 
RsrII CG'GwC_CG

Cuts at:   2361    2361 
   Size:       4166
 
SacI G_AGCT'C

Cuts at:   1180    1180 
   Size:       4166
 
SalI G'TCGA_C

Cuts at:    313    1203     313 
   Size:        890    3276
 
SapI GCTCTTCn'nnn_

Cuts at:    194    2189    2399     194 
   Size:       1995     210    1961

  Fragments arranged by size: 

                1995    1961     210
 
SbfI CC_TGCA'GG

Cuts at:   1213    1213 
   Size:       4166
 
ScaI AGT'ACT

Cuts at:    367    3683     367 
   Size:       3316     850
 
SfcI C'TryA_G

Cuts at:     33     683    1209    1894    2887    3078      33 
   Size:        650     526     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     650     526     191
 
SfoI GGC'GCC

Cuts at:   1598    1846    1598 
   Size:        248    3918
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4166
 
SmaI CCC'GGG

Cuts at:   1188    1188 
   Size:       4166
 
SmlI C'TyrA_G

Cuts at:   1321    2236    2728    2990    1321 
   Size:        915     492     262    2497

  Fragments arranged by size: 

                2497     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4166
 
SphI G_CATG'C

Cuts at:   1219    1719    1219 
   Size:        500    3666
 
StyI C'CwwG_G

Cuts at:    107    1145    1300    1654     107 
   Size:       1038     155     354    2619

  Fragments arranged by size: 

                2619    1038     354     155
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1749    1882    2341    2550    1749 
   Size:        133     459     209    3365

  Fragments arranged by size: 

                3365     459     209     133
 
TatI w'GTAC_w

Cuts at:    365     518     959    3681     365 
   Size:        153     441    2722     850

  Fragments arranged by size: 

                2722     850     441     153
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3683    3715    3683 
   Size:         32    4134
 
Tth111I GACn'n_nGTC

Cuts at:   1963    1963 
   Size:       4166
 
XbaI T'CTAG_A

Cuts at:   1197    1197 
   Size:       4166
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1661    1661 
   Size:       4166
 
XmaI C'CCGG_G

Cuts at:   1186    1186 
   Size:       4166
 
XmnI GAAnn'nnTTC

Cuts at:    219     367     794     219 
   Size:        148     427    3591

  Fragments arranged by size: 

                3591     427     148
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI     BpmI   Bpu10I    BsaXI    BseRI    BsiWI     BsmI    BsmBI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI     HpaI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI
     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI
     ZraI
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