Restriction map for pF1KB0403
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AflIII      AgeI        Alw44I      AlwI        AocI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BcgI        BciVI       
BfrI        BlnI        BsaAI       BsaBI       BsaWI       
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DrdI        Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        MamI        MfeI        MluI        MroI        
MseI        MunI        NarI        NdeI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TfiI        Tru9I       Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII         241  GACGTC               GACGT^C
AccI          244  GTCTAC               GT^[AC][GT]AC
AhdI         1171  GACCTCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       821  AGCGCT               AGC^GCT
ApaI         1001  GGGCCC               GGGCC^C
Asp700I        57  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1171  GACCTCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          566  TGGCCA               TGG^CCA
BanII        1001  GGGCCC               G[GA]GC[CT]^C
BbsI          238  GAAGAC               GAAGAC(2/6)
BclI          311  TGATCA               T^GATCA
BglI          890  GCCGCTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         668  AGATCT               A^GATCT
BlpI          125  GCTCAGC              GC^T[ACGT]AGC
BpmI          538  CTGGAG               CTGGAG(16/14)
Bpu1102I      125  GCTCAGC              GC^T[ACGT]AGC
BpuAI         238  GAAGAC               GAAGAC(2/6)
BsiEI         204  CGGCCG               CG[GA][CT]^CG
BsrDI        1197  CATTGC               GCAATG(2/0)
BstAPI        899  GCAGCGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         668  AGATCT               [GA]^GATC[CT]
CelII         125  GCTCAGC              GC^T[ACGT]AGC
DraIII        744  CACTCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          204  CGGCCG               C^GGCCG
Eam1105I     1171  GACCTCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          180  GAAGAG               CTCTTC(1/4)
EclXI         204  CGGCCG               C^GGCCG
Eco47III      821  AGCGCT               AGC^GCT
Eco52I        204  CGGCCG               C^GGCCG
EcoNI        1236  CCTGGCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          311  TGATCA               T^GATCA
HinfI         103  GACTC                G^A[ACGT]TC
HphI          314  TCACC                GGTGA(8/7)
Ksp632I       180  GAAGAG               CTCTTC(1/4)
MflI          668  AGATCT               [GA]^GATC[CT]
MluNI         566  TGGCCA               TGG^CCA
MscI          566  TGGCCA               TGG^CCA
NaeI          521  GCCGGC               GCC^GGC
NcoI         1139  CCATGG               C^CATGG
NgoMIV        521  GCCGGC               G^CCGGC
NheI          805  GCTAGC               G^CTAGC
PleI          103  GACTC                GAGTC(4/5)
XhoII         668  AGATCT               [GA]^GATC[CT]
XmnI           57  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AlwNI         900  CAGCGACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1063  CAGAGGCTG           
AspHI         123  GTGCTC               G[AT]GC[AT]^C
              640  GAGCAC              
AvaI          225  CTCGGG               C^[CT]CG[GA]G
              287  CCCGAG              
BbvCI        1189  GCTGAGG              CCTCAGC(-5/-2)
             1240  GCTGAGG             
BsaI         1385  GAGACC               GGTCTC(1/5)
              654  GGTCTC              
BsiHKAI       123  GTGCTC               G[AT]GC[AT]^C
              640  GAGCAC              
BsoBI         225  CTCGGG               C^[CT]CG[GA]G
              287  CCCGAG              
BspMI         298  ACCTGC               ACCTGC(4/8)
              230  GCAGGT              
BsrFI         521  GCCGGC               [GA]^CCGG[CT]
             1208  ACCGGC              
Cfr10I        521  GCCGGC               [GA]^CCGG[CT]
             1208  ACCGGC              
DraII         611  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1232  AGGACCT             
EaeI          204  CGGCCG               [CT]^GGCC[GA]
              566  TGGCCA              
EcoO109I      611  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1232  AGGACCT             
EcoT14I       575  CCTTGG               C^C[AT][AT]GG
             1139  CCATGG              
KspI          918  CCGCGG               CCGC^GG
             1253  CCGCGG              
MaeIII        674  GTGAC                ^GT[ACGT]AC
             1052  GTGAC               
MslI         1134  CACTCCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1197  CATTGCCATG          
NspI          140  ACATGC               [GA]CATG^[CT]
              947  ACATGC              
PpuMI         611  GGGACCT              [GA]G^G[AT]CC[CT]
             1232  AGGACCT             
SacII         918  CCGCGG               CCGC^GG
             1253  CCGCGG              
SexAI         467  ACCTGGT              A^CC[AT]GGT
              650  ACCAGGT             
StuI          788  AGGCCT               AGG^CCT
              926  AGGCCT              
StyI          575  CCTTGG               C^C[AT][AT]GG
             1139  CCATGG              
Tsp45I        674  GTGAC                ^GT[GC]AC
             1052  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AvaII       BbvI        BcnI        BfaI        BmyI        
BsaHI       BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DsaI        EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HpaII       ItaI        MaeI        
MaeII       MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TseI        TspRI       TthHB8I     
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