Restriction map for pF1KB0403
 (Linear) (Six-base) MAPSORT of: KIBB0403.seq  Check: 3474  from: 1  to: 1395



KIBB0403.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AatII G_ACGT'C

Cuts at:      0     245    1395 
   Size:        245    1150
 
AccI GT'mk_AC

Cuts at:      0     245    1395 
   Size:        245    1150
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10     777    1395 
   Size:         10     767     618

  Fragments arranged by size: 

                 767     618      10
 
AfeI AGC'GCT

Cuts at:      0     823    1395 
   Size:        823     572
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1176    1395 
   Size:       1176     219
 
AlwNI CAG_nnn'CTG

Cuts at:      0     905    1068    1395 
   Size:        905     163     327

  Fragments arranged by size: 

                 905     327     163
 
ApaI G_GGCC'C

Cuts at:      0    1005    1395 
   Size:       1005     390
 
AvaI C'yCGr_G

Cuts at:      0     225     287    1395 
   Size:        225      62    1108

  Fragments arranged by size: 

                1108     225      62
 
BanII G_rGCy'C

Cuts at:      0    1005    1395 
   Size:       1005     390
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     245    1395 
   Size:        245    1150
 
BbvCI CC'TCA_GC

Cuts at:      0    1190    1241    1395 
   Size:       1190      51     154

  Fragments arranged by size: 

                1190     154      51
 
BclI T'GATC_A

Cuts at:      0     311    1395 
   Size:        311    1084
 
BglI GCCn_nnn'nGGC

Cuts at:      0     896    1395 
   Size:        896     499
 
BglII A'GATC_T

Cuts at:      0     668    1395 
   Size:        668     727
 
BlpI GC'TnA_GC

Cuts at:      0     126    1395 
   Size:        126    1269
 
Bme1580I G_kGCm'C

Cuts at:      0    1005    1395 
   Size:       1005     390
 
BmgBI CAC'GTC

Cuts at:      0     564    1178    1395 
   Size:        564     614     217

  Fragments arranged by size: 

                 614     564     217
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1008    1314    1363    1395 
   Size:       1008     306      49      32

  Fragments arranged by size: 

                1008     306      49      32
 
BmtI G_CTAG'C

Cuts at:      0     809    1395 
   Size:        809     586
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     559    1395 
   Size:        559     836
 
Bpu10I CC'TnA_GC

Cuts at:      0     502    1190    1241    1395 
   Size:        502     688      51     154

  Fragments arranged by size: 

                 688     502     154      51
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     660    1379    1395 
   Size:        660     719      16

  Fragments arranged by size: 

                 719     660      16
 
BsaHI Gr'CG_yC

Cuts at:      0     173     242     845    1055    1395 
   Size:        173      69     603     210     340

  Fragments arranged by size: 

                 603     340     210     173      69
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     382     412     640     670     961     991    1018    1048 
   Size:        382      30     228      30     291      30      27      30

Cuts at:   1048    1395 
   Size:        347

  Fragments arranged by size: 

                 382     347     291     228      30      30      30      30
                  27
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     463     466     814    1258    1261    1395 
   Size:        463       3     348     444       3     134

  Fragments arranged by size: 

                 463     444     348     134       3       3
 
BseYI C'CCAG_C

Cuts at:      0     267     911    1220    1395 
   Size:        267     644     309     175

  Fragments arranged by size: 

                 644     309     267     175
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     313     577    1395 
   Size:         25     288     264     818

  Fragments arranged by size: 

                 818     288     264      25
 
BsiEI CG_ry'CG

Cuts at:      0     207    1395 
   Size:        207    1188
 
BsiHKAI G_wGCw'C

Cuts at:      0     127     644    1395 
   Size:        127     517     751

  Fragments arranged by size: 

                 751     517     127
 
Bsp1286I G_dGCh'C

Cuts at:      0     127     644    1005    1395 
   Size:        127     517     361     390

  Fragments arranged by size: 

                 517     390     361     127
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     221     307    1395 
   Size:        221      86    1088

  Fragments arranged by size: 

                1088     221      86
 
BsrDI GCAATG_nn'

Cuts at:      0    1196    1395 
   Size:       1196     199
 
BsrFI r'CCGG_y

Cuts at:      0     521    1208    1395 
   Size:        521     687     187

  Fragments arranged by size: 

                 687     521     187
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     905    1395 
   Size:        905     490
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     552     576    1044    1140    1395 
   Size:        552      24     468      96     255

  Fragments arranged by size: 

                 552     468     255      96      24
 
BstYI r'GATC_y

Cuts at:      0     668    1395 
   Size:        668     727
 
BtgI C'CryG_G

Cuts at:      0      96     918    1139    1253    1395 
   Size:         96     822     221     114     142

  Fragments arranged by size: 

                 822     221     142     114      96
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     585    1395 
   Size:        585     810
 
BtsI GCAGTG_nn'

Cuts at:      0     127    1395 
   Size:        127    1268
 
DraIII CAC_nnn'GTG

Cuts at:      0     749    1395 
   Size:        749     646
 
EaeI y'GGCC_r

Cuts at:      0     204     566    1395 
   Size:        204     362     829

  Fragments arranged by size: 

                 829     362     204
 
EagI C'GGCC_G

Cuts at:      0     204    1395 
   Size:        204    1191
 
EarI CTCTTCn'nnn_

Cuts at:      0     175    1395 
   Size:        175    1220
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     775    1395 
   Size:        775     620
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10     559     777    1395 
   Size:         10     549     218     618

  Fragments arranged by size: 

                 618     549     218      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1240    1395 
   Size:       1240     155
 
EcoO109I rG'GnC_Cy

Cuts at:      0     612    1233    1395 
   Size:        612     621     162

  Fragments arranged by size: 

                 621     612     162
 
HaeII r_GCGC'y

Cuts at:      0     281     522     575     825    1395 
   Size:        281     241      53     250     570

  Fragments arranged by size: 

                 570     281     250     241      53
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     208     240     482     514    1018    1050    1162    1194 
   Size:        208      32     242      32     504      32     112      32

Cuts at:   1194    1395 
   Size:        201

  Fragments arranged by size: 

                 504     242     208     201     112      32      32      32
                  32
 
MscI TGG'CCA

Cuts at:      0     568    1395 
   Size:        568     827
 
MslI CAynn'nnrTG

Cuts at:      0    1138    1201    1395 
   Size:       1138      63     194

  Fragments arranged by size: 

                1138     194      63
 
MspA1I CmG'CkG

Cuts at:      0     252     692     869     893     920    1160    1215    1255 
   Size:        252     440     177      24      27     240      55      40

Cuts at:   1255    1305    1338    1395 
   Size:         50      33      57

  Fragments arranged by size: 

                 440     252     240     177      57      55      50      40
                  33      27      24
 
NaeI GCC'GGC

Cuts at:      0     523    1395 
   Size:        523     872
 
NcoI C'CATG_G

Cuts at:      0    1139    1395 
   Size:       1139     256
 
NgoMIV G'CCGG_C

Cuts at:      0     521    1395 
   Size:        521     874
 
NheI G'CTAG_C

Cuts at:      0     805    1395 
   Size:        805     590
 
NspI r_CATG'y

Cuts at:      0     144     951    1395 
   Size:        144     807     444

  Fragments arranged by size: 

                 807     444     144
 
PasI CC'CwG_GG

Cuts at:      0    1097    1395 
   Size:       1097     298
 
PfoI T'CCnGG_A

Cuts at:      0     167     965    1395 
   Size:        167     798     430

  Fragments arranged by size: 

                 798     430     167
 
PpuMI rG'GwC_Cy

Cuts at:      0     612    1233    1395 
   Size:        612     621     162

  Fragments arranged by size: 

                 621     612     162
 
PspOMI G'GGCC_C

Cuts at:      0    1001    1395 
   Size:       1001     394
 
PstI C_TGCA'G

Cuts at:      0     122     257    1091    1151    1304    1395 
   Size:        122     135     834      60     153      91

  Fragments arranged by size: 

                 834     153     135     122      91      60
 
PvuII CAG'CTG

Cuts at:      0     252    1160    1215    1305    1395 
   Size:        252     908      55      90      90

  Fragments arranged by size: 

                 908     252      90      90      55
 
SacII CC_GC'GG

Cuts at:      0     921    1256    1395 
   Size:        921     335     139

  Fragments arranged by size: 

                 921     335     139
 
SexAI A'CCwGG_T

Cuts at:      0     467     650    1395 
   Size:        467     183     745

  Fragments arranged by size: 

                 745     467     183
 
SfcI C'TryA_G

Cuts at:      0     118     253    1087    1147    1300    1395 
   Size:        118     135     834      60     153      95

  Fragments arranged by size: 

                 834     153     135     118      95      60
 
StuI AGG'CCT

Cuts at:      0     790     928    1395 
   Size:        790     138     467

  Fragments arranged by size: 

                 790     467     138
 
StyI C'CwwG_G

Cuts at:      0     575    1139    1395 
   Size:        575     564     256

  Fragments arranged by size: 

                 575     564     256
 
TatI w'GTAC_w

Cuts at:      0     764    1395 
   Size:        764     631
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1146    1178    1395 
   Size:       1146      32     217

  Fragments arranged by size: 

                1146     217      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      61    1395 
   Size:         61    1334
 
ZraI GAC'GTC

Cuts at:      0     243    1395 
   Size:        243    1152
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AfeI     AhdI    AlwNI     ApaI     AvaI
    BanII     BbsI    BbvCI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I     BsaI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrDI
    BsrFI   BstAPI    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NheI
     NspI     PasI     PfoI    PpuMI   PspOMI     PstI    PvuII    SacII
    SexAI     SfcI     StuI     StyI     TatI     TstI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI    AflII   AflIII     AgeI     AleI     AlfI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI     BcgI    BciVI    BfrBI     BplI    BpuEI
    BsaAI    BsaBI    BsaWI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I
     ClaI    CspCI     DraI     DrdI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NarI     NdeI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII  Tth111I
     XbaI     XcmI     XhoI     XmaI
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