Restriction map for pF1KB8560
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsoBI       BspDI       
BspEI       BspLU11I    BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FauI        
FseI        FspI        HaeII       HapII       HhaI        
Hin1I       HinP1I      HpaI        HpaII       KasI        
KpnI        KspI        MaeII       MfeI        MluI        
MluNI       MroI        MscI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SacII       SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    StuI        Tth111I     Van91I      
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AccI         2377  GTCGAC               GT^[AC][GT]AC
AciI         1954  GCGG                 CCGC(-3/-1)
BanII        1757  GAGCTC               G[GA]GC[CT]^C
BciVI        1996  GTATCC               GTATCC(6/5)
BclI         1164  TGATCA               T^GATCA
BglII        2330  AGATCT               A^GATCT
BseRI         632  GAGGAG               GAGGAG(10/8)
BsmBI           3  GAGACG               CGTCTC(1/5)
BsmI          654  GCATTC               GAATGC(1/-1)
BspHI        2469  TCATGA               T^CATGA
BspMI         614  GCAGGT               ACCTGC(4/8)
BstF5I        852  GGATG                GGATG(2/0)
DraII        1038  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO109I     1038  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       335  CCAAGG               C^C[AT][AT]GG
FbaI         1164  TGATCA               T^GATCA
FokI          852  GGATG                GGATG(9/13)
HaeIII        541  GGCC                 GG^CC
HgaI         2043  GCGTC                GACGC(5/10)
HindIII      1092  AAGCTT               A^AGCTT
PpuMI        1038  GGGACCC              [GA]G^G[AT]CC[CT]
RcaI         2469  TCATGA               T^CATGA
SacI         1757  GAGCTC               GAGCT^C
SalI         2377  GTCGAC               G^TCGAC
SapI         1541  GAAGAGC              GCTCTTC(1/4)
SmlI         2714  CTCAAG               C^T[CT][GA]AG
StyI          335  CCAAGG               C^C[AT][AT]GG
SwaI         2642  ATTTAAAT             ATTT^AAAT


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AlwNI        1704  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2231  CAGCCACTG           
AseI          145  ATTAAT               AT^TAAT
             1416  ATTAAT              
AsnI          145  ATTAAT               AT^TAAT
             1416  ATTAAT              
AspHI        1655  GAGCAC               G[AT]GC[AT]^C
             1757  GAGCTC              
BbsI          993  GAAGAC               GAAGAC(2/6)
             1068  GAAGAC              
BmyI         1655  GAGCAC               G[AGT]GC[ACT]^C
             1757  GAGCTC              
BpuAI         993  GAAGAC               GAAGAC(2/6)
             1068  GAAGAC              
BsaBI         805  GATAAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2221  GATTGGAATC          
BsaJI         335  CCAAGG               C^C[ACGT][ACGT]GG
             1247  CCCTGG              
BsiHKAI      1655  GAGCAC               G[AT]GC[AT]^C
             1757  GAGCTC              
BsiYI         843  CCCTCTTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1042  CCCTATGGAGG         
BslI          843  CCCTCTTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1042  CCCTATGGAGG         
BsmAI           3  GAGAC                GTCTC(1/5)
             2539  GTCTC               
Bsp1286I     1655  GAGCAC               G[AGT]GC[ACT]^C
             1757  GAGCTC              
Bsp1407I     2286  TGTACA               T^GTACA
             2491  TGTACA              
BsrGI        2286  TGTACA               T^GTACA
             2491  TGTACA              
Cac8I        1052  GCAAGC               GC[ACGT]^[ACGT]GC
             1865  GCTTGC              
DraI          192  TTTAAA               TTT^AAA
             2643  TTTAAA              
EcoRI          36  GAATTC               G^AATTC
             1186  GAATTC              
HincII       2123  GTCAAC               GT[CT]^[GA]AC
             2377  GTCGAC              
HindII       2123  GTCAAC               GT[CT]^[GA]AC
             2377  GTCGAC              
MamI          805  GATAAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2221  GATTGGAATC          
MspA1I       1547  CAGCTG               C[AC]G^C[GT]G
             2608  CAGCTG              
MwoI          752  GCAAACCACGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              964  GCTATTCTTGC         
PshBI         145  ATTAAT               AT^TAAT
             1416  ATTAAT              
PstI           11  CTGCAG               CTGCA^G
             1027  CTGCAG              
PvuII        1547  CAGCTG               CAG^CTG
             2608  CAGCTG              
ScaI          214  AGTACT               AGT^ACT
              640  AGTACT              
SfaNI         430  GATGC                GCATC(5/9)
             1299  GCATC               
SspBI        2286  TGTACA               T^GTACA
             2491  TGTACA              
XbaI         2254  TCTAGA               T^CTAGA
             2542  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
Asp700I     AvaII       BbvI        BfaI        BpmI        
BsmFI       BsrDI       BsrI        BssKI       BstNI       
BstYI       Cfr13I      DdeI        DpnII       EarI        
EcoRII      Fnu4HI      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MvaI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        SspI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI        
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