Restriction map for pF1KB8560
 (Linear) (Six-base) MAPSORT of: KIBB8560.seq  Check: 7428  from: 1  to: 2730



KIBB8560.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     605    2730 
   Size:        605    2125
 
AccI GT'mk_AC

Cuts at:      0    2378    2730 
   Size:       2378     352
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     588    2632    2730 
   Size:        588    2044      98

  Fragments arranged by size: 

                2044     588      98
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2489    2521    2730 
   Size:       2489      32     209

  Fragments arranged by size: 

                2489     209      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1709    2236    2730 
   Size:       1709     527     494

  Fragments arranged by size: 

                1709     527     494
 
ApoI r'AATT_y

Cuts at:      0      36      55     471     511     780    1117    1186    1270 
   Size:         36      19     416      40     269     337      69      84

Cuts at:   1270    1405    1689    2430    2530    2730 
   Size:        135     284     741     100     200

  Fragments arranged by size: 

                 741     416     337     284     269     200     135     100
                  84      69      40      36      19
 
AseI AT'TA_AT

Cuts at:      0     146    1417    2730 
   Size:        146    1271    1313

  Fragments arranged by size: 

                1313    1271     146
 
BanII G_rGCy'C

Cuts at:      0    1761    2730 
   Size:       1761     969
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1000    1075    2730 
   Size:       1000      75    1655

  Fragments arranged by size: 

                1655    1000      75
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2007    2730 
   Size:       2007     723
 
BclI T'GATC_A

Cuts at:      0    1164    2730 
   Size:       1164    1566
 
BglII A'GATC_T

Cuts at:      0    2330    2730 
   Size:       2330     400
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     578    2654    2730 
   Size:         50     528    2076      76

  Fragments arranged by size: 

                2076     528      76      50
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2699    2730 
   Size:       2699      31
 
BsaBI GATnn'nnATC

Cuts at:      0     809    2225    2730 
   Size:        809    1416     505

  Fragments arranged by size: 

                1416     809     505
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1365    1395    2256    2286    2489    2519    2730 
   Size:       1365      30     861      30     203      30     211

  Fragments arranged by size: 

                1365     861     211     203      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     647    2730 
   Size:        647    2083
 
BseYI C'CCAG_C

Cuts at:      0    2606    2730 
   Size:       2606     124
 
BsiHKAI G_wGCw'C

Cuts at:      0    1659    1761    2730 
   Size:       1659     102     969

  Fragments arranged by size: 

                1659     969     102
 
BsmI GAATG_Cn'

Cuts at:      0     654    2730 
   Size:        654    2076
 
Bsp1286I G_dGCh'C

Cuts at:      0    1659    1761    2730 
   Size:       1659     102     969

  Fragments arranged by size: 

                1659     969     102
 
BspHI T'CATG_A

Cuts at:      0    2469    2730 
   Size:       2469     261
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     605    2730 
   Size:        605    2125
 
BsrDI GCAATG_nn'

Cuts at:      0     997    1009    2255    2623    2730 
   Size:        997      12    1246     368     107

  Fragments arranged by size: 

                1246     997     368     107      12
 
BsrGI T'GTAC_A

Cuts at:      0    2286    2491    2730 
   Size:       2286     205     239

  Fragments arranged by size: 

                2286     239     205
 
BstYI r'GATC_y

Cuts at:      0     288    2194    2330    2655    2730 
   Size:        288    1906     136     325      75

  Fragments arranged by size: 

                1906     325     288     136      75
 
BtsI GCAGTG_nn'

Cuts at:      0     252     335     615    1036    2730 
   Size:        252      83     280     421    1694

  Fragments arranged by size: 

                1694     421     280     252      83
 
DraI TTT'AAA

Cuts at:      0     194    2645    2730 
   Size:        194    2451      85

  Fragments arranged by size: 

                2451     194      85
 
EarI CTCTTCn'nnn_

Cuts at:      0      89     341     489     530    1536    2136    2730 
   Size:         89     252     148      41    1006     600     594

  Fragments arranged by size: 

                1006     600     594     252     148      89      41
 
EcoICRI GAG'CTC

Cuts at:      0    1759    2730 
   Size:       1759     971
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     578     588    2632    2654    2730 
   Size:         50     528      10    2044      22      76

  Fragments arranged by size: 

                2044     528      76      50      22      10
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1039    2730 
   Size:       1039    1691
 
EcoRI G'AATT_C

Cuts at:      0      36    1186    2730 
   Size:         36    1150    1544

  Fragments arranged by size: 

                1544    1150      36
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1562    1594    2168    2200    2256    2288    2730 
   Size:       1562      32     574      32      56      32     442

  Fragments arranged by size: 

                1562     574     442      56      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2125    2379    2730 
   Size:       2125     254     351

  Fragments arranged by size: 

                2125     351     254
 
HindIII A'AGCT_T

Cuts at:      0    1092    2730 
   Size:       1092    1638
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1706    2024    2504    2730 
   Size:       1706     318     480     226

  Fragments arranged by size: 

                1706     480     318     226
 
MslI CAynn'nnrTG

Cuts at:      0    1002    2051    2513    2730 
   Size:       1002    1049     462     217

  Fragments arranged by size: 

                1049    1002     462     217
 
MspA1I CmG'CkG

Cuts at:      0    1549    2610    2730 
   Size:       1549    1061     120

  Fragments arranged by size: 

                1549    1061     120
 
PfoI T'CCnGG_A

Cuts at:      0      66    1434    2730 
   Size:         66    1368    1296

  Fragments arranged by size: 

                1368    1296      66
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2489    2521    2730 
   Size:       2489      32     209

  Fragments arranged by size: 

                2489     209      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1039    2730 
   Size:       1039    1691
 
PsiI TTA'TAA

Cuts at:      0     509    1603    2730 
   Size:        509    1094    1127

  Fragments arranged by size: 

                1127    1094     509
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     699     731    2730 
   Size:        699      32    1999

  Fragments arranged by size: 

                1999     699      32
 
PstI C_TGCA'G

Cuts at:      0      15    1031    2730 
   Size:         15    1016    1699

  Fragments arranged by size: 

                1699    1016      15
 
PvuII CAG'CTG

Cuts at:      0    1549    2610    2730 
   Size:       1549    1061     120

  Fragments arranged by size: 

                1549    1061     120
 
SacI G_AGCT'C

Cuts at:      0    1761    2730 
   Size:       1761     969
 
SalI G'TCGA_C

Cuts at:      0    2377    2730 
   Size:       2377     353
 
SanDI GG'GwC_CC

Cuts at:      0    1039    2730 
   Size:       1039    1691
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1536    2730 
   Size:       1536    1194
 
ScaI AGT'ACT

Cuts at:      0     216     642    2730 
   Size:        216     426    2088

  Fragments arranged by size: 

                2088     426     216
 
SfcI C'TryA_G

Cuts at:      0      11    1027    1331    1660    1930    2309    2730 
   Size:         11    1016     304     329     270     379     421

  Fragments arranged by size: 

                1016     421     379     329     304     270      11
 
SmlI C'TyrA_G

Cuts at:      0    2714    2730 
   Size:       2714      16
 
SspI AAT'ATT

Cuts at:      0     256     373    1442    2730 
   Size:        256     117    1069    1288

  Fragments arranged by size: 

                1288    1069     256     117
 
StyI C'CwwG_G

Cuts at:      0     335    2730 
   Size:        335    2395
 
SwaI ATTT'AAAT

Cuts at:      0    2645    2730 
   Size:       2645      85
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     571    2730 
   Size:        571    2159
 
TatI w'GTAC_w

Cuts at:      0     214     640    2286    2491    2730 
   Size:        214     426    1646     205     239

  Fragments arranged by size: 

                1646     426     239     214     205
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1082    1114    2730 
   Size:       1082      32    1616

  Fragments arranged by size: 

                1616    1082      32
 
XbaI T'CTAG_A

Cuts at:      0    2254    2542    2730 
   Size:       2254     288     188

  Fragments arranged by size: 

                2254     288     188
 
XmnI GAAnn'nnTTC

Cuts at:      0      40     679     739     838    2402    2504    2730 
   Size:         40     639      60      99    1564     102     226

  Fragments arranged by size: 

                1564     639     226     102      99      60      40
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AloI    AlwNI     ApoI     AseI    BanII
     BbsI    BciVI     BclI    BglII     BpmI    BpuEI    BsaBI    BsaXI
    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
    BsrGI    BstYI     BtsI     DraI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI    Hin4I   HincII  HindIII     MmeI     MslI   MspA1I     PfoI
     PpiI    PpuMI     PsiI     PsrI     PstI    PvuII     SacI     SalI
    SanDI     SapI     ScaI     SfcI     SmlI     SspI     StyI     SwaI
    TaqII     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
    PshAI   PspOMI    PspXI     PvuI    RsrII    SacII     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI  Tth111I     XcmI     XhoI     XmaI     ZraI
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