Restriction map for pF1KB8550
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AhdI        Alw44I      AocI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       BalI        BamHI       
BcgI        BclI        BfrI        BglII       BlpI        
Bpu1102I    BseAI       BsiWI       BsmBI       Bsp1407I    
BspEI       BspLU11I    BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KpnI        KspI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PflMI       PmeI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     Van91I      XbaI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI          436  GTCTAC               GT^[AC][GT]AC
AccII          42  CGCG                 CG^CG
AgeI           28  ACCGGT               A^CCGGT
Aor51HI      2395  AGCGCT               AGC^GCT
ApaI          305  GGGCCC               GGGCC^C
AspHI        1016  GAGCTC               G[AT]GC[AT]^C
AvrII          36  CCTAGG               C^CTAGG
BbeI          739  GGCGCC               GGCGC^C
BbsI         1582  GTCTTC               GAAGAC(2/6)
BglI         1894  GCCAATCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI           36  CCTAGG               C^CTAGG
BpuAI        1582  GTCTTC               GAAGAC(2/6)
BsiEI         453  CGACCG               CG[GA][CT]^CG
BsiHKAI      1016  GAGCTC               G[AT]GC[AT]^C
BsmI          954  GAATGC               GAATGC(1/-1)
BspDI        2664  ATCGAT               AT^CGAT
BspHI        1547  TCATGA               T^CATGA
BsrBI         589  GAGCGG               CCGCTC(-3/-3)
BsrFI          28  ACCGGT               [GA]^CCGG[CT]
BstUI          42  CGCG                 CG^CG
Cfr10I         28  ACCGGT               [GA]^CCGG[CT]
ClaI         2664  ATCGAT               AT^CGAT
Eco47III     2395  AGCGCT               AGC^GCT
KasI          739  GGCGCC               G^GCGCC
MvnI           42  CGCG                 CG^CG
NarI          739  GGCGCC               GG^CGCC
PaeR7I        824  CTCGAG               C^TCGAG
PinAI          28  ACCGGT               A^CCGGT
PshAI         516  GACAGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1547  TCATGA               T^CATGA
SacI         1016  GAGCTC               GAGCT^C
SapI         1451  GAAGAGC              GCTCTTC(1/4)
SfoI          739  GGCGCC               GGC^GCC
StuI         1415  AGGCCT               AGG^CCT
XcmI          979  CCAGCTGTTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          824  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI          430  AAATTT               [GA]^AATT[CT]
              724  AAATTC              
ApoI          430  AAATTT               [GA]^AATT[CT]
              724  AAATTC              
AvaI          824  CTCGAG               C^[CT]CG[GA]G
             1397  CCCGAG              
BanI           52  GGTGCC               G^G[CT][GA]CC
              739  GGCGCC              
BbrPI        1099  CACGTG               CAC^GTG
             1345  CACGTG              
BbvCI        2406  CCTCAGC              CCTCAGC(-5/-2)
              901  GCTGAGG             
BciVI         486  GGATAC               GTATCC(6/5)
              550  GGATAC              
BsaAI        1099  CACGTG               [CT]AC^GT[GA]
             1345  CACGTG              
BsaBI        1002  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1539  GATCTCCATC          
BsaWI          28  ACCGGT               [AT]^CCGG[AT]
              554  ACCGGA              
BsoBI         824  CTCGAG               C^[CT]CG[GA]G
             1397  CCCGAG              
BspMI         510  GCAGGT               ACCTGC(4/8)
             1026  GCAGGT              
BstAPI       1023  GCAGCAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1330  GCAGAGATTGC         
BstXI        1340  CCAAGCACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2201  CCATGAAGGTGG        
DsaI         1916  CCGTGG               C^C[GA][CT]GG
             2708  CCACGG              
EaeI          788  CGGCCA               [CT]^GGCC[GA]
             1901  TGGCCG              
HaeII         739  GGCGCC               [GA]GCGC^[CT]
             2395  AGCGCT              
HincII        280  GTCAAC               GT[CT]^[GA]AC
              523  GTCAAC              
HindII        280  GTCAAC               GT[CT]^[GA]AC
              523  GTCAAC              
MamI         1002  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1539  GATCTCCATC          
NspI          114  ACATGC               [GA]CATG^[CT]
              284  ACATGC              
PleI          242  GAGTC                GAGTC(4/5)
             2155  GAGTC               
PmaCI        1099  CACGTG               CAC^GTG
             1345  CACGTG              
PmlI         1099  CACGTG               CAC^GTG
             1345  CACGTG              
SmlI          824  CTCGAG               C^T[CT][GA]AG
             1264  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AvaII       BanII       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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