Restriction map for pF1KB8550
 (Linear) (Six-base) MAPSORT of: KIBB8550.seq  Check: 9565  from: 1  to: 2718



KIBB8550.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     501    1017    2718 
   Size:        501     516    1701

  Fragments arranged by size: 

                1701     516     501
 
AccI GT'mk_AC

Cuts at:      0     437    2718 
   Size:        437    2281
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352    1662    2001    2718 
   Size:        352    1310     339     717

  Fragments arranged by size: 

                1310     717     352     339
 
AfeI AGC'GCT

Cuts at:      0    2397    2718 
   Size:       2397     321
 
AgeI A'CCGG_T

Cuts at:      0      28    2718 
   Size:         28    2690
 
AleI CACnn'nnGTG

Cuts at:      0     838    2718 
   Size:        838    1880
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      29    2718 
   Size:         29    2689
 
AlwNI CAG_nnn'CTG

Cuts at:      0     496     573     918    2718 
   Size:        496      77     345    1800

  Fragments arranged by size: 

                1800     496     345      77
 
ApaI G_GGCC'C

Cuts at:      0     309    2718 
   Size:        309    2409
 
ApoI r'AATT_y

Cuts at:      0     430     724    2718 
   Size:        430     294    1994

  Fragments arranged by size: 

                1994     430     294
 
AvaI C'yCGr_G

Cuts at:      0     824    1397    2718 
   Size:        824     573    1321

  Fragments arranged by size: 

                1321     824     573
 
AvrII C'CTAG_G

Cuts at:      0      36    2718 
   Size:         36    2682
 
BanI G'GyrC_C

Cuts at:      0      52     739    2718 
   Size:         52     687    1979

  Fragments arranged by size: 

                1979     687      52
 
BanII G_rGCy'C

Cuts at:      0     155     309    1020    1398    2718 
   Size:        155     154     711     378    1320

  Fragments arranged by size: 

                1320     711     378     155     154
 
BbeI G_GCGC'C

Cuts at:      0     743    2718 
   Size:        743    1975
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1575    2718 
   Size:       1575    1143
 
BbvCI CC'TCA_GC

Cuts at:      0     902    2407    2718 
   Size:        902    1505     311

  Fragments arranged by size: 

                1505     902     311
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     480     544    2718 
   Size:        480      64    2174

  Fragments arranged by size: 

                2174     480      64
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1900    2718 
   Size:       1900     818
 
Bme1580I G_kGCm'C

Cuts at:      0     309    2718 
   Size:        309    2409
 
BmgBI CAC'GTC

Cuts at:      0     315    2718 
   Size:        315    2403
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     222    2718 
   Size:        222    2496
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     595    1387    2173    2718 
   Size:        595     792     786     545

  Fragments arranged by size: 

                 792     786     595     545
 
Bpu10I CC'TnA_GC

Cuts at:      0     902    2407    2718 
   Size:        902    1505     311

  Fragments arranged by size: 

                1505     902     311
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1249    2718 
   Size:       1249    1469
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     821     846    1394    2718 
   Size:        821      25     548    1324

  Fragments arranged by size: 

                1324     821     548      25
 
BsaAI yAC'GTr

Cuts at:      0    1101    1347    2718 
   Size:       1101     246    1371

  Fragments arranged by size: 

                1371    1101     246
 
BsaBI GATnn'nnATC

Cuts at:      0    1006    1543    2718 
   Size:       1006     537    1175

  Fragments arranged by size: 

                1175    1006     537
 
BsaHI Gr'CG_yC

Cuts at:      0     740    1077    1850    2718 
   Size:        740     337     773     868

  Fragments arranged by size: 

                 868     773     740     337
 
BsaWI w'CCGG_w

Cuts at:      0      28     554    2718 
   Size:         28     526    2164

  Fragments arranged by size: 

                2164     526      28
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1427    1457    1637    1667    2718 
   Size:       1427      30     180      30    1051

  Fragments arranged by size: 

                1427    1051     180      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     559    1872    2116    2718 
   Size:        559    1313     244     602

  Fragments arranged by size: 

                1313     602     559     244
 
BseYI C'CCAG_C

Cuts at:      0     958     978    1189    2718 
   Size:        958      20     211    1529

  Fragments arranged by size: 

                1529     958     211      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       9    1078    1967    2718 
   Size:          9    1069     889     751

  Fragments arranged by size: 

                1069     889     751       9
 
BsiEI CG_ry'CG

Cuts at:      0     456    2718 
   Size:        456    2262
 
BsiHKAI G_wGCw'C

Cuts at:      0    1020    2718 
   Size:       1020    1698
 
BsmI GAATG_Cn'

Cuts at:      0     960    2718 
   Size:        960    1758
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     309    1020    1398    2718 
   Size:        155     154     711     378    1320

  Fragments arranged by size: 

                1320     711     378     155     154
 
BspHI T'CATG_A

Cuts at:      0    1547    2718 
   Size:       1547    1171
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     501    1017    2718 
   Size:        501     516    1701

  Fragments arranged by size: 

                1701     516     501
 
BsrBI CCG'CTC

Cuts at:      0     591    2718 
   Size:        591    2127
 
BsrDI GCAATG_nn'

Cuts at:      0    1299    2240    2418    2718 
   Size:       1299     941     178     300

  Fragments arranged by size: 

                1299     941     300     178
 
BsrFI r'CCGG_y

Cuts at:      0      28    2718 
   Size:         28    2690
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1029    1336    2718 
   Size:       1029     307    1382

  Fragments arranged by size: 

                1382    1029     307
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1347    2208    2718 
   Size:       1347     861     510

  Fragments arranged by size: 

                1347     861     510
 
BstYI r'GATC_y

Cuts at:      0    1248    1538    1999    2718 
   Size:       1248     290     461     719

  Fragments arranged by size: 

                1248     719     461     290
 
BtgI C'CryG_G

Cuts at:      0    1916    2708    2718 
   Size:       1916     792      10

  Fragments arranged by size: 

                1916     792      10
 
BtsI GCAGTG_nn'

Cuts at:      0     811    2294    2619    2718 
   Size:        811    1483     325      99

  Fragments arranged by size: 

                1483     811     325      99
 
ClaI AT'CG_AT

Cuts at:      0    2665    2718 
   Size:       2665      53
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     717     752    2718 
   Size:        717      35    1966

  Fragments arranged by size: 

                1966     717      35
 
EaeI y'GGCC_r

Cuts at:      0     788    1901    2718 
   Size:        788    1113     817

  Fragments arranged by size: 

                1113     817     788
 
EarI CTCTTCn'nnn_

Cuts at:      0     844    1446    1666    1697    2718 
   Size:        844     602     220      31    1021

  Fragments arranged by size: 

                1021     844     602     220      31
 
EcoICRI GAG'CTC

Cuts at:      0    1018    2718 
   Size:       1018    1700
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     595    1387    1662    2001    2173    2718 
   Size:        352     243     792     275     339     172     545

  Fragments arranged by size: 

                 792     545     352     339     275     243     172
 
EcoO109I rG'GnC_Cy

Cuts at:      0     305     306     570    1823    1953    2718 
   Size:        305       1     264    1253     130     765

  Fragments arranged by size: 

                1253     765     305     264     130       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2031    2718 
   Size:       2031     687
 
HaeII r_GCGC'y

Cuts at:      0     743    2399    2718 
   Size:        743    1656     319

  Fragments arranged by size: 

                1656     743     319
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     598     630    1427    1459    1917    1949    2382    2414 
   Size:        598      32     797      32     458      32     433      32

Cuts at:   2414    2718 
   Size:        304

  Fragments arranged by size: 

                 797     598     458     433     304      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     282     525    2718 
   Size:        282     243    2193

  Fragments arranged by size: 

                2193     282     243
 
KasI G'GCGC_C

Cuts at:      0     739    2718 
   Size:        739    1979
 
MslI CAynn'nnrTG

Cuts at:      0     838    1645    2206    2718 
   Size:        838     807     561     512

  Fragments arranged by size: 

                 838     807     561     512
 
MspA1I CmG'CkG

Cuts at:      0     372     493     762     982    2184    2366    2588    2606 
   Size:        372     121     269     220    1202     182     222      18

Cuts at:   2606    2718 
   Size:        112

  Fragments arranged by size: 

                1202     372     269     222     220     182     121     112
                  18
 
NarI GG'CG_CC

Cuts at:      0     740    2718 
   Size:        740    1978
 
NspI r_CATG'y

Cuts at:      0     118     288    2718 
   Size:        118     170    2430

  Fragments arranged by size: 

                2430     170     118
 
PasI CC'CwG_GG

Cuts at:      0     989    1782    2718 
   Size:        989     793     936

  Fragments arranged by size: 

                 989     936     793
 
PfoI T'CCnGG_A

Cuts at:      0     482    1493    2150    2718 
   Size:        482    1011     657     568

  Fragments arranged by size: 

                1011     657     568     482
 
PmlI CAC'GTG

Cuts at:      0    1101    1347    2718 
   Size:       1101     246    1371

  Fragments arranged by size: 

                1371    1101     246
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2670    2702    2718 
   Size:       2670      32      16

  Fragments arranged by size: 

                2670      32      16
 
PpuMI rG'GwC_Cy

Cuts at:      0     570    1823    1953    2718 
   Size:        570    1253     130     765

  Fragments arranged by size: 

                1253     765     570     130
 
PshAI GACnn'nnGTC

Cuts at:      0     520    2718 
   Size:        520    2198
 
PsiI TTA'TAA

Cuts at:      0     649    2718 
   Size:        649    2069
 
PspOMI G'GGCC_C

Cuts at:      0     305    2718 
   Size:        305    2413
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1207    1239    2718 
   Size:       1207      32    1479

  Fragments arranged by size: 

                1479    1207      32
 
PstI C_TGCA'G

Cuts at:      0     389     476    1025    1173    2718 
   Size:        389      87     549     148    1545

  Fragments arranged by size: 

                1545     549     389     148      87
 
PvuII CAG'CTG

Cuts at:      0     372     493     762     982    2718 
   Size:        372     121     269     220    1736

  Fragments arranged by size: 

                1736     372     269     220     121
 
SacI G_AGCT'C

Cuts at:      0    1020    2718 
   Size:       1020    1698
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1446    2718 
   Size:       1446    1272
 
SfcI C'TryA_G

Cuts at:      0     213     385     472    1021    1169    2718 
   Size:        213     172      87     549     148    1549

  Fragments arranged by size: 

                1549     549     213     172     148      87
 
SfoI GGC'GCC

Cuts at:      0     741    2718 
   Size:        741    1977
 
SmlI C'TyrA_G

Cuts at:      0     824    1264    2718 
   Size:        824     440    1454

  Fragments arranged by size: 

                1454     824     440
 
StuI AGG'CCT

Cuts at:      0    1417    2718 
   Size:       1417    1301
 
StyI C'CwwG_G

Cuts at:      0      36     996    2252    2381    2699    2718 
   Size:         36     960    1256     129     318      19

  Fragments arranged by size: 

                1256     960     318     129      36      19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2265    2718 
   Size:       2265     453
 
TatI w'GTAC_w

Cuts at:      0     860    2171    2718 
   Size:        860    1311     547

  Fragments arranged by size: 

                1311     860     547
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     801     833    2178    2210    2718 
   Size:        801      32    1345      32     508

  Fragments arranged by size: 

                1345     801     508      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     986    2718 
   Size:        986    1732
 
XhoI C'TCGA_G

Cuts at:      0     824    2718 
   Size:        824    1894
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AgeI     AleI     AlfI    AlwNI
     ApaI     ApoI     AvaI    AvrII     BanI    BanII     BbeI     BbsI
    BbvCI    BciVI     BglI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BstAPI    BstXI    BstYI     BtgI     BtsI
     ClaI    CspCI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FalI
    HaeII    Hin4I   HincII     KasI     MslI   MspA1I     NarI     NspI
     PasI     PfoI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
     PsrI     PstI    PvuII     SacI     SapI     SfcI     SfoI     SmlI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AhdI     AloI    ApaLI
     AscI     AseI    AsiSI     BaeI    BamHI     BcgI     BclI    BfrBI
    BglII     BlpI     BmtI     BplI    BsiWI    BsmBI    BspEI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     DraI
   DraIII     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PmeI    PspXI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XbaI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com