Restriction map for pF1KB6115
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BbeI        BbrPI       
BciVI       BfaI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspEI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     CelII       
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       EcoT22I     
FseI        FspI        HindIII     HpaI        KasI        
KpnI        MaeI        MfeI        MluI        MroI        
MseI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
Tsp509I     Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcyI          921  GGCGTC               G[GA]^CG[CT]C
AocI          782  CCTCAGG              CC^T[ACGT]AGG
ApaI           72  GGGCCC               GGGCC^C
BamHI         530  GGATCC               G^GATCC
BbvCI        1211  CCTCAGC              CCTCAGC(-5/-2)
BcgI          191  GCAAGAAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1289  TGATCA               T^GATCA
BglII         398  AGATCT               A^GATCT
BsaAI         820  TACGTG               [CT]AC^GT[GA]
BsaHI         921  GGCGTC               G[GA]^CG[CT]C
BsaI          181  GAGACC               GGTCTC(1/5)
BspDI        1303  ATCGAT               AT^CGAT
BstAPI       1122  GCACAGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        852  GGTCACC              G^GT[ACGT]ACC
BstPI         852  GGTCACC              G^GT[ACGT]ACC
Bsu36I        782  CCTCAGG              CC^T[ACGT]AGG
ClaI         1303  ATCGAT               AT^CGAT
DraIII       1123  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1033  GACGCGATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        782  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       852  GGTCACC              G^GT[ACGT]ACC
FauI          147  CCCGC                CCCGC(4/6)
FbaI         1289  TGATCA               T^GATCA
Hin1I         921  GGCGTC               G[GA]^CG[CT]C
HincII        898  GTTGAC               GT[CT]^[GA]AC
HindII        898  GTTGAC               GT[CT]^[GA]AC
NaeI         1438  GCCGGC               GCC^GGC
NgoMIV       1438  GCCGGC               G^CCGGC
PflMI         242  CCAACGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1753  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI          26  GACTCATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1740  CTGCAG               CTGCA^G
PvuII        1315  CAGCTG               CAG^CTG
ScaI          644  AGTACT               AGT^ACT
SmlI         1852  CTCAAG               C^T[CT][GA]AG
Van91I        242  CCAACGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AvaI          603  CTCGGG               C^[CT]CG[GA]G
             1789  CCCGAG              
BanI          286  GGTGCC               G^G[CT][GA]CC
             1713  GGTGCC              
BsaBI         571  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1167  GATCTTCATC          
BseRI         504  CTCCTC               GAGGAG(10/8)
             1074  CTCCTC              
BsmAI         181  GAGAC                GTCTC(1/5)
              596  GAGAC               
BsoBI         603  CTCGGG               C^[CT]CG[GA]G
             1789  CCCGAG              
BspMI         305  ACCTGC               ACCTGC(4/8)
              633  ACCTGC              
BsrDI         321  CATTGC               GCAATG(2/0)
             1782  CATTGC              
BsrFI         908  ACCGGC               [GA]^CCGG[CT]
             1438  GCCGGC              
BstYI         398  AGATCT               [GA]^GATC[CT]
              530  GGATCC              
Cfr10I        908  ACCGGC               [GA]^CCGG[CT]
             1438  GCCGGC              
DraII         699  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             1753  GGGTCCT             
EcoNI          96  CCTTGTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              762  CCTCTGGAAGG         
EcoO109I      699  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             1753  GGGTCCT             
HaeII         486  GGCGCT               [GA]GCGC^[CT]
             1264  AGCGCC              
KspI          148  CCGCGG               CCGC^GG
              706  CCGCGG              
MamI          571  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1167  GATCTTCATC          
MflI          398  AGATCT               [GA]^GATC[CT]
              530  GGATCC              
SacII         148  CCGCGG               CCGC^GG
              706  CCGCGG              
SfcI         1026  CTACAG               C^T[GA][CT]AG
             1740  CTGCAG              
XhoII         398  AGATCT               [GA]^GATC[CT]
              530  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaII       BalI        BanII       
BbsI        BbvI        BcnI        BmyI        BpmI        
BpuAI       BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EaeI        EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PleI        
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     XcmI        
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