Restriction map for pF1KB8528
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# NO CUT ENZYMES (   88 enzymes) #
##################################

AccI        AclI        AflII       AgeI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     AviII       AvrII       BamHI       
BbrPI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BsaAI       BsaI        
BsiEI       BsiWI       BsmI        BspDI       BspHI       
BssHII      Bst1107I    BstEII      BstPI       BstXI       
BstZ17I     ClaI        DraI        DraIII      EagI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoRV       FbaI        FseI        FspI        
HindIII     HpaI        KspI        MfeI        MluI        
MslI        MunI        NdeI        NheI        NotI        
NruI        PacI        PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SgrAI       SnaBI       
SphI        Sse8387I    SspI        StuI        SwaI        
XbaI        XcmI        XmnI        


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# ONE CUT ENZYMES (   61 enzymes) #
###################################

AatII         694  GACGTC               GACGT^C
Acc65I       1942  GGTACC               G^GTACC
AccII        1372  CGCG                 CG^CG
AccIII       1848  TCCGGA               T^CCGGA
AhdI         2303  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1531  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I      1942  GGTACC               G^GTACC
AspEI        2303  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2535  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         2644  TGGCCA               TGG^CCA
BanII          22  GAGCCC               G[GA]GC[CT]^C
BbeI            3  GGCGCC               GGCGC^C
BbvCI         547  CCTCAGC              CCTCAGC(-5/-2)
BsaWI        1848  TCCGGA               [AT]^CCGG[AT]
BseAI        1848  TCCGGA               T^CCGGA
BseRI        1291  GAGGAG               GAGGAG(10/8)
BsmBI        2112  CGTCTC               CGTCTC(1/5)
Bsp1407I     1046  TGTACA               T^GTACA
BspEI        1848  TCCGGA               T^CCGGA
BsrBI        1776  GAGCGG               CCGCTC(-3/-3)
BsrDI        2556  CATTGC               GCAATG(2/0)
BsrFI         430  GCCGGC               [GA]^CCGG[CT]
BsrGI        1046  TGTACA               T^GTACA
BssSI         889  CTCGTG               CACGAG(-5/-1)
BstBI        1034  TTCGAA               TT^CGAA
BstUI        1372  CGCG                 CG^CG
BstYI        1879  GGATCT               [GA]^GATC[CT]
Cfr10I        430  GCCGGC               [GA]^CCGG[CT]
CpoI         1779  CGGACCG              CG^G[AT]CCG
DrdI          688  GACTTGGACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         2644  TGGCCA               [CT]^GGCC[GA]
Eam1105I     2303  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I      2553  ATGCAT               ATGCA^T
FauI          660  GCGGG                CCCGC(4/6)
HaeII           3  GGCGCC               [GA]GCGC^[CT]
HgaI           91  GACGC                GACGC(5/10)
HphI          137  TCACC                GGTGA(8/7)
KasI            3  GGCGCC               G^GCGCC
KpnI         1942  GGTACC               GGTAC^C
MflI         1879  GGATCT               [GA]^GATC[CT]
MluNI        2644  TGGCCA               TGG^CCA
MroI         1848  TCCGGA               T^CCGGA
MscI         2644  TGGCCA               TGG^CCA
MvnI         1372  CGCG                 CG^CG
NaeI          430  GCCGGC               GCC^GGC
NarI            3  GGCGCC               GG^CGCC
NcoI          270  CCATGG               C^CATGG
NgoMIV        430  GCCGGC               G^CCGGC
NsiI         2553  ATGCAT               ATGCA^T
NspV         1034  TTCGAA               TT^CGAA
PaeR7I       1027  CTCGAG               C^TCGAG
PflFI        2535  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RsrII        1779  CGGACCG              CG^G[AT]CCG
SapI         1773  GAAGAGC              GCTCTTC(1/4)
SfoI            3  GGCGCC               GGC^GCC
SfuI         1034  TTCGAA               TT^CGAA
SpeI          336  ACTAGT               A^CTAGT
SspBI        1046  TGTACA               T^GTACA
Tth111I      2535  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1027  CTCGAG               C^TCGAG
XhoII        1879  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AspHI         943  GAGCAC               G[AT]GC[AT]^C
             2105  GTGCTC              
BanI            3  GGCGCC               G^G[CT][GA]CC
             1942  GGTACC              
BlpI         1299  GCTCAGC              GC^T[ACGT]AGC
             2230  GCTGAGC             
Bpu1102I     1299  GCTCAGC              GC^T[ACGT]AGC
             2230  GCTGAGC             
BsaBI        1162  GATACTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1852  GATGGCTATC          
BsgI          704  CTGCAC               GTGCAG(16/14)
             1390  GTGCAG              
BsiHKAI       943  GAGCAC               G[AT]GC[AT]^C
             2105  GTGCTC              
CelII        1299  GCTCAGC              GC^T[ACGT]AGC
             2230  GCTGAGC             
DsaI          125  CCGTGG               C^C[GA][CT]GG
              270  CCATGG              
HincII        934  GTTGAC               GT[CT]^[GA]AC
             1518  GTTGAC              
HindII        934  GTTGAC               GT[CT]^[GA]AC
             1518  GTTGAC              
MamI         1162  GATACTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1852  GATGGCTATC          
PflMI         160  CCACAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1870  CCAACCAGTGG         
PstI         1396  CTGCAG               CTGCA^G
             2276  CTGCAG              
TfiI          408  GAATC                G^A[AT]TC
             1105  GATTC               
Van91I        160  CCACAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1870  CCAACCAGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflIII      
AluI        AlwI        AocI        ApoI        AvaI        
AvaII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspLU11I    BspMI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SmlI        StyI        TaqI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XmaCI       XmaI        
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