Restriction map for pF1KB8528
 (Linear) (Six-base) MAPSORT of: KIBB8528.seq  Check: 3815  from: 1  to: 2655



KIBB8528.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1389    2655 
   Size:       1389    1266
 
AatII G_ACGT'C

Cuts at:      0     698    2655 
   Size:        698    1957
 
Acc65I G'GTAC_C

Cuts at:      0    1942    2655 
   Size:       1942     713
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226    1643    1772    2018    2616    2655 
   Size:        226    1417     129     246     598      39

  Fragments arranged by size: 

                1417     598     246     226     129      39
 
AflIII A'CryG_T

Cuts at:      0     755    1043    1154    2655 
   Size:        755     288     111    1501

  Fragments arranged by size: 

                1501     755     288     111
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2308    2655 
   Size:       2308     347
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     277    2258    2655 
   Size:        277    1981     397

  Fragments arranged by size: 

                1981     397     277
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1536    2655 
   Size:       1536    1119
 
ApoI r'AATT_y

Cuts at:      0     925     989    1438    2399    2655 
   Size:        925      64     449     961     256

  Fragments arranged by size: 

                 961     925     449     256      64
 
AvaI C'yCGr_G

Cuts at:      0      17    1027    1288    1310    1727    2246    2655 
   Size:         17    1010     261      22     417     519     409

  Fragments arranged by size: 

                1010     519     417     409     261      22      17
 
BanI G'GyrC_C

Cuts at:      0       3    1942    2655 
   Size:          3    1939     713

  Fragments arranged by size: 

                1939     713       3
 
BanII G_rGCy'C

Cuts at:      0      26    2655 
   Size:         26    2629
 
BbeI G_GCGC'C

Cuts at:      0       7    2655 
   Size:          7    2648
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     326    1013    2006    2655 
   Size:        326     687     993     649

  Fragments arranged by size: 

                 993     687     649     326
 
BbvCI CC'TCA_GC

Cuts at:      0     548    2655 
   Size:        548    2107
 
BfrBI ATG'CAT

Cuts at:      0    2555    2655 
   Size:       2555     100
 
BlpI GC'TnA_GC

Cuts at:      0    1300    2231    2655 
   Size:       1300     931     424

  Fragments arranged by size: 

                1300     931     424
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       5     825    1663    1784    2104    2655 
   Size:          5     820     838     121     320     551

  Fragments arranged by size: 

                 838     820     551     320     121       5
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     966    2655 
   Size:        966    1689
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382    1378    1566    2655 
   Size:        382     996     188    1089

  Fragments arranged by size: 

                1089     996     382     188
 
Bpu10I CC'TnA_GC

Cuts at:      0     548    2655 
   Size:        548    2107
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     538     623    1417    1729    2655 
   Size:        538      85     794     312     926

  Fragments arranged by size: 

                 926     794     538     312      85
 
BsaBI GATnn'nnATC

Cuts at:      0    1166    1856    2655 
   Size:       1166     690     799

  Fragments arranged by size: 

                1166     799     690
 
BsaHI Gr'CG_yC

Cuts at:      0       4      92     695    2655 
   Size:          4      88     603    1960

  Fragments arranged by size: 

                1960     603      88       4
 
BsaWI w'CCGG_w

Cuts at:      0    1848    2655 
   Size:       1848     807
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      56      86    1819    1849    2655 
   Size:         56      30    1733      30     806

  Fragments arranged by size: 

                1733     806      56      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1306    2655 
   Size:       1306    1349
 
BseYI C'CCAG_C

Cuts at:      0     737     798    1074    1938    2655 
   Size:        737      61     276     864     717

  Fragments arranged by size: 

                 864     737     717     276      61
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     689    1411    2655 
   Size:        689     722    1244

  Fragments arranged by size: 

                1244     722     689
 
BsiHKAI G_wGCw'C

Cuts at:      0     947    2109    2655 
   Size:        947    1162     546

  Fragments arranged by size: 

                1162     947     546
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2118    2655 
   Size:       2118     537
 
Bsp1286I G_dGCh'C

Cuts at:      0      26     947    2109    2655 
   Size:         26     921    1162     546

  Fragments arranged by size: 

                1162     921     546      26
 
BspEI T'CCGG_A

Cuts at:      0    1848    2655 
   Size:       1848     807
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     429     732     985    1337    1389    2298    2655 
   Size:        429     303     253     352      52     909     357

  Fragments arranged by size: 

                 909     429     357     352     303     253      52
 
BsrBI CCG'CTC

Cuts at:      0    1778    2655 
   Size:       1778     877
 
BsrDI GCAATG_nn'

Cuts at:      0    2555    2655 
   Size:       2555     100
 
BsrFI r'CCGG_y

Cuts at:      0     430    2655 
   Size:        430    2225
 
BsrGI T'GTAC_A

Cuts at:      0    1046    2655 
   Size:       1046    1609
 
BssSI C'ACGA_G

Cuts at:      0     889    2655 
   Size:        889    1766
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1389    2313    2552    2655 
   Size:       1389     924     239     103

  Fragments arranged by size: 

                1389     924     239     103
 
BstBI TT'CG_AA

Cuts at:      0    1035    2655 
   Size:       1035    1620
 
BstYI r'GATC_y

Cuts at:      0    1879    2655 
   Size:       1879     776
 
Bsu36I CC'TnA_GG

Cuts at:      0     393     683    1634    1643    2655 
   Size:        393     290     951       9    1012

  Fragments arranged by size: 

                1012     951     393     290       9
 
BtgI C'CryG_G

Cuts at:      0     125     270    2655 
   Size:        125     145    2385

  Fragments arranged by size: 

                2385     145     125
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     185    2655 
   Size:        185    2470
 
BtsI GCAGTG_nn'

Cuts at:      0     815    2655 
   Size:        815    1840
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     694    2655 
   Size:        694    1961
 
EaeI y'GGCC_r

Cuts at:      0    2644    2655 
   Size:       2644      11
 
EarI CTCTTCn'nnn_

Cuts at:      0     845    1768    1915    2329    2655 
   Size:        845     923     147     414     326

  Fragments arranged by size: 

                 923     845     414     326     147
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     382    1378    1566    1643    1772    2018    2616 
   Size:        226     156     996     188      77     129     246     598

Cuts at:   2616    2655 
   Size:         39

  Fragments arranged by size: 

                 996     598     246     226     188     156     129      77
                  39
 
EcoO109I rG'GnC_Cy

Cuts at:      0      30     153     441    2418    2655 
   Size:         30     123     288    1977     237

  Fragments arranged by size: 

                1977     288     237     123      30
 
HaeII r_GCGC'y

Cuts at:      0       7    2655 
   Size:          7    2648
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     376     408     536     568    2294    2326    2655 
   Size:        376      32     128      32    1726      32     329

  Fragments arranged by size: 

                1726     376     329     128      32      32      32
 
HincII GTy'rAC

Cuts at:      0     936    1520    2655 
   Size:        936     584    1135

  Fragments arranged by size: 

                1135     936     584
 
KasI G'GCGC_C

Cuts at:      0       3    2655 
   Size:          3    2652
 
KpnI G_GTAC'C

Cuts at:      0    1946    2655 
   Size:       1946     709
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     567     605    2149    2325    2632    2655 
   Size:         83     484      38    1544     176     307      23

  Fragments arranged by size: 

                1544     484     307     176      83      38      23
 
MscI TGG'CCA

Cuts at:      0    2646    2655 
   Size:       2646       9
 
MspA1I CmG'CkG

Cuts at:      0     464    1260    1395    1533    1785    1966    2230    2242 
   Size:        464     796     135     138     252     181     264      12

Cuts at:   2242    2655 
   Size:        413

  Fragments arranged by size: 

                 796     464     413     264     252     181     138     135
                  12
 
NaeI GCC'GGC

Cuts at:      0     432    2655 
   Size:        432    2223
 
NarI GG'CG_CC

Cuts at:      0       4    2655 
   Size:          4    2651
 
NcoI C'CATG_G

Cuts at:      0     270    2655 
   Size:        270    2385
 
NgoMIV G'CCGG_C

Cuts at:      0     430    2655 
   Size:        430    2225
 
NsiI A_TGCA'T

Cuts at:      0    2557    2655 
   Size:       2557      98
 
NspI r_CATG'y

Cuts at:      0     759    1047    1158    1952    2601    2655 
   Size:        759     288     111     794     649      54

  Fragments arranged by size: 

                 794     759     649     288     111      54
 
PasI CC'CwG_GG

Cuts at:      0      26    2655 
   Size:         26    2629
 
PciI A'CATG_T

Cuts at:      0     755    1043    1154    2655 
   Size:        755     288     111    1501

  Fragments arranged by size: 

                1501     755     288     111
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     166    1876    2655 
   Size:        166    1710     779

  Fragments arranged by size: 

                1710     779     166
 
PfoI T'CCnGG_A

Cuts at:      0      86    2655 
   Size:         86    2569
 
PpuMI rG'GwC_Cy

Cuts at:      0      30     441    2418    2655 
   Size:         30     411    1977     237

  Fragments arranged by size: 

                1977     411     237      30
 
PspXI vC'TCGA_Gb

Cuts at:      0    1027    2655 
   Size:       1027    1628
 
PstI C_TGCA'G

Cuts at:      0    1400    2280    2655 
   Size:       1400     880     375

  Fragments arranged by size: 

                1400     880     375
 
PvuII CAG'CTG

Cuts at:      0     464    1260    1395    1533    1966    2230    2242    2655 
   Size:        464     796     135     138     433     264      12     413

  Fragments arranged by size: 

                 796     464     433     413     264     138     135      12
            
 
RsrII CG'GwC_CG

Cuts at:      0    1780    2655 
   Size:       1780     875
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1768    2655 
   Size:       1768     887
 
SfcI C'TryA_G

Cuts at:      0     845    1123    1396    1482    2276    2457    2655 
   Size:        845     278     273      86     794     181     198

  Fragments arranged by size: 

                 845     794     278     273     198     181      86
 
SfoI GGC'GCC

Cuts at:      0       5    2655 
   Size:          5    2650
 
SmaI CCC'GGG

Cuts at:      0      19    1312    2248    2655 
   Size:         19    1293     936     407

  Fragments arranged by size: 

                1293     936     407      19
 
SmlI C'TyrA_G

Cuts at:      0     553     638    1027    1432    1744    2655 
   Size:        553      85     389     405     312     911

  Fragments arranged by size: 

                 911     553     405     389     312      85
 
SpeI A'CTAG_T

Cuts at:      0     336    2655 
   Size:        336    2319
 
StyI C'CwwG_G

Cuts at:      0     140     270    2359    2655 
   Size:        140     130    2089     296

  Fragments arranged by size: 

                2089     296     140     130
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      24     263    1336    2655 
   Size:         24     239    1073    1319

  Fragments arranged by size: 

                1319    1073     239      24
 
TatI w'GTAC_w

Cuts at:      0    1046    1590    2190    2655 
   Size:       1046     544     600     465

  Fragments arranged by size: 

                1046     600     544     465
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      56      88    1819    1851    2655 
   Size:         56      32    1731      32     804

  Fragments arranged by size: 

                1731     804      56      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2538    2655 
   Size:       2538     117
 
XhoI C'TCGA_G

Cuts at:      0    1027    2655 
   Size:       1027    1628
 
XmaI C'CCGG_G

Cuts at:      0      17    1310    2246    2655 
   Size:         17    1293     936     409

  Fragments arranged by size: 

                1293     936     409      17
 
ZraI GAC'GTC

Cuts at:      0     696    2655 
   Size:        696    1959
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI   AflIII     AhdI     AlfI    AlwNI
     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI    BfrBI
     BlpI     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DrdI     EaeI
     EarI  Eco57MI EcoO109I    HaeII    Hin4I   HincII     KasI     KpnI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI
     NspI     PasI     PciI    PflMI     PfoI    PpuMI    PspXI     PstI
    PvuII    RsrII     SapI     SfcI     SfoI     SmaI     SmlI     SpeI
     StyI    TaqII     TatI     TstI  Tth111I     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI    AflII     AgeI     AleI     AloI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI
    BciVI     BclI     BglI    BglII Bme1580I    BmgBI     BmtI     BsaI
    BsaAI    BsiEI    BsiWI     BsmI    BspHI   BssHII   BstEII    BstXI
  BstZ17I     ClaI    CspCI     DraI   DraIII     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     HpaI     MfeI     MluI     MslI     NdeI     NheI     NotI     NruI
     PacI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI     PsrI
     PvuI     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SphI     SrfI     SspI     StuI
     SwaI     XbaI     XcmI     XmnI
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